https://notulaebotanicae.ro/index.php/nbha/issue/feed Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-12-10T11:15:21+00:00 Radu E. SESTRAS rsestras@notulaebotanicae.ro Open Journal Systems <p style="text-align: justify;"><strong>Notulae Botanicae Horti Agrobotanici Cluj-Napoca</strong> is a peer-reviewed&nbsp;<strong>quarterly (beginning in 2019)</strong> journal aimed at disseminating significant research and original papers, critical reviews and short reviews. The subjects refer on plant biodiversity, genetics and plant breeding, development of new methodologies that can be of interest to a wide audience of plant scientists in all areas of plant biology, agriculture, horticulture and forestry. The journal encourages authors to frame their research questions and discuss their results in terms of the major questions of plant sciences, thereby maximizing the impact and value of their research, and thus in favor of spreading their studies outcome. The papers must be of potential interest to a significant number of scientists and, if specific to a local situation, must be relevant to a wide body of knowledge in life sciences. Articles should make a significant contribution to the advancement of knowledge or toward a better understanding of existing biological and agricultural concepts. An international Editorial Board advises the journal. The total content of the journal may be used for educational, non-profit purposes without regard to copyright. The distribution of the material is encouraged with the condition that the authors and the source (<strong>Notulae Botanicae Horti Agrobotanici Cluj-Napoca</strong> or JCR abbrev. title <strong>Not Bot Horti Agrobo</strong>) are mentioned.</p> <p style="text-align: justify;"><strong>Editor-in-Chief</strong></p> <ul> <li class="show"><a href="https://www.sestras.ro/">Radu E. SESTRAS</a></li> </ul> <p><strong>Advisory Editors</strong></p> <ul> <li class="show">Cornel CATOI</li> <li class="show">Viorel MITRE</li> <li class="show">Doru C. PAMFIL</li> </ul> <p><a href="/index.php/nbha/about/editorialTeam" target="_blank" rel="noopener"><strong>Editorial Board</strong></a></p> <p><strong>Journal Secretary</strong></p> <ul> <li class="show">Roxana L. STOIAN</li> <li class="show">Silviu L. MIC</li> </ul> <p>&nbsp;</p> <p>Print <strong>ISSN 0255-965X; </strong>Electronic <strong>ISSN 1842-4309</strong></p> <p><strong>License: &nbsp;</strong><strong> <a title="CC BY 4.0" href="https://creativecommons.org/licenses/by/4.0/" target="_blank" rel="noopener"><img src="/public/site/images/silviu_mic/by1.png" width="80" height="28"></a></strong><strong>&nbsp; <a title="About The Licenses" href="https://creativecommons.org/licenses/" target="_blank" rel="noopener"><img src="/public/site/images/silviu_mic/cc.logo_.png" width="118" height="28"></a></strong></p> <p>&nbsp;</p> <p><strong>Open Access Journal:</strong> Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.</p> <p>&nbsp;</p> <ul> <li class="show">See information on <strong>‘</strong><strong>Policies’</strong> (<a href="/index.php/nbha/focus-scope">Focus and Scope</a>; <a href="/index.php/nbha/about/editorialPolicies#peerReviewProcess">Peer Review Process</a>; <a href="/index.php/nbha/about/editorialPolicies#publicationFrequency">Publication Frequency</a>; <a href="/index.php/nbha/about/editorialPolicies#openAccessPolicy">Open Access Policy</a>; <a href="/index.php/nbha/about/editorialPolicies#custom-0">Publication Ethics and Publication Malpractice Policy</a> etc.); <strong>‘</strong><strong>Submissions’</strong> (<a href="/index.php/nbha/about/submissions#onlineSubmissions">Online Submissions</a>; <a href="/index.php/nbha/about/submissions#authorGuidelines">Author Guidelines</a>; <a href="/index.php/nbha/about/submissions#copyrightNotice">Copyright Notice</a>; <a href="/index.php/nbha/about/submissions#privacyStatement">Privacy Statement</a>; <a href="/index.php/nbha/about/submissions#authorFees">Payment</a>&nbsp;etc.).</li> </ul> <p>&nbsp;</p> <ul> <li class="show"><strong>June 1, 2018: </strong>Notulae Botanicae Horti Agrobotanici Cluj-Napoca in <strong><span style="color: #297439;"><a class="cta-secondary" style="color: #297439;" title="CiteScore 2018 value" href="https://journalmetrics.scopus.com/" target="_blank" rel="noopener noreferrer">CiteScore rank (Scopus – Elsevier)</a></span></strong><strong> <span style="color: #297439;"><a href="https://www.scopus.com/sourceid/17700156420?origin=sbrowse">#34/72 in Horticulture</a> </span></strong></li> <li class="show"><strong>June 26, 2018: </strong>Notulae Botanicae Horti Agrobotanici Cluj-Napoca in<strong> <span style="color: #297439;"><a href="https://clarivate.com/blog/science-research-connect/the-2018-jcr-release-is-here/"><span class="cta-secondary" style="color: #297439;">ISI Clarivate Analytics</span> 183/222 in Plant Sciences</a></span></strong><strong><span style="color: #297439;">&nbsp;</span></strong></li> </ul> <p align="center">&nbsp;</p> <p align="center"><strong>Published by AcademicPres on behalf of:</strong></p> <p align="center">University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca</p> <p align="center">Romanian Academy of Agricultural and Forestry Sciences</p> <p align="center">Horticulture and Forestry Society from Transylvania</p> <p align="center">&nbsp;</p> <p class="functie"><strong>Abstracted and/or indexed:</strong></p> <ul> <li class="show">Clarivate Analytics, Master Journal List - SCIE, JCR, <a href="http://mjl.clarivate.com/">http://mjl.clarivate.com/</a>&nbsp;(IF 2017=0.648)</li> <li class="show">Scopus - Elsevier, <a href="https://www.scopus.com/sourceid/17700156420">https://www.scopus.com/sourceid/17700156420</a> (CiteScore 2017, metrics 2018=0.78)</li> <li class="show">ProQuest,&nbsp;<a href="http://www.proquest.com/">http://www.proquest.com/</a></li> <li class="show">Centre for Agriculture and Biosciences International (CABI),&nbsp;<a href="https://www.cabdirect.org/">https://www.cabdirect.org/</a></li> <li class="show">Chemical Abstracts Service (CAS),&nbsp;<a href="https://www.cas.org/">https://www.cas.org/</a></li> <li class="show">Russian Scientific and Technical Information Institute (VINITI),&nbsp;<a href="http://www.viniti.ru/">http://www.viniti.ru</a></li> <li class="show">Bielefeld Academic Search Engine (BASE),&nbsp;<a href="http://www.base-search.net/">http://www.base-search.net</a></li> <li class="show">Google Scholar,&nbsp;<a href="http://scholar.google.com/">http://scholar.google.com</a></li> <li class="show">British Library Direct (BLD),&nbsp;<a href="http://explore.bl.uk/">http://explore.bl.uk/</a></li> <li class="show">Mendeley,&nbsp;<a href="https://www.mendeley.com/research-papers/?query=Notulae%20Botanicae%20Horti%20Agrobotanici%20Cluj-Napoca">https://www.mendeley.com</a></li> <li class="show">The European Library,&nbsp;<a href="http://www.theeuropeanlibrary.org/tel4/search?query=Notulae%20Botanicae%20Horti%20Agrobotanici%20Cluj-Napoca">http://www.theeuropeanlibrary.org</a></li> <li class="show">Harvard Library,&nbsp;<a href="http://hollis.harvard.edu/primo_library/libweb/action/display.do?tabs=detailsTab&amp;ct=display&amp;fn=search&amp;doc=HVD_ALEPH011313468&amp;indx=1&amp;recIds=HVD_ALEPH011313468&amp;recIdxs=0&amp;elementId=0&amp;renderMode=poppedOut&amp;displayMode=full&amp;frbrVersion=&amp;frbg=&amp;&amp;vl(51615747UI0)=title&amp;dscnt=0&amp;vl(1UIStartWith0)=contains&amp;scp.scps=scope%3A%28HVD_FGDC%29%2Cscope%3A%28HVD%29%2Cscope%3A%28HVD_VIA%29&amp;tb=t&amp;mode=Basic&amp;vid=HVD&amp;tab=books&amp;srt=rank&amp;vl(394521272UI1)=all_items&amp;vl(freeText0)=Notulae%20Botanicae%20Horti%20Agrobotanici%20Cluj-Napoca&amp;dum=true&amp;dstmp=1510572056817">http://hollis.harvard.edu</a></li> <li class="show">Tianjin University of Finance and Economics (TJUFE),&nbsp;<a href="http://en.tjufe.findplus.cn/?h=search_list&amp;query=Notulae%20Botanicae%20Horti%20Agrobotanici%20Cluj-Napoca&amp;action[addexpander][]=fulltext">http://en.tjufe.findplus.cn</a></li> <li class="show">Open Archives Initiative Protocol for Metadata Harvesting (OAIster),&nbsp;<a href="http://www.oaister.org/">http://www.oaister.org</a></li> <li class="show">China Educational Publications Import and Export Corporation (SOCOLAR),&nbsp;<a href="http://www.socolar.com/">http://www.socolar.com</a></li> <li class="show">Ulrich's Periodicals Directory,&nbsp;<a href="http://www.ulrichsweb.com/">http://www.ulrichsweb.com</a></li> <li class="show">Elton B Stephens COmpany (EBSCO),&nbsp;<a href="http://www.ebscohost.com/">http://www.ebscohost.com</a></li> <li class="show">Information Matrix for the Analysis of Journals (MIAR),&nbsp;<a href="http://miar.ub.edu/">http://miar.ub.edu/</a></li> </ul> <div>&nbsp;</div> <p align="center"><em>First published 1965</em></p> <p align="center"><em>Print ISSN 0255-965X, Electronic ISSN 1842-4309</em></p> <p class="subpunct" align="center"><strong><em><a href="/index.php/nbha">www.notulaebotanicae.ro</a></em></strong></p> https://notulaebotanicae.ro/index.php/nbha/article/view/11349 Triticum aestivum Assay - A Useful Tool for Environmental Monitoring and Toxicity Assessment 2019-12-04T14:25:41+00:00 Alexandra JITĂREANU jitareanu.alexandra@umfiasi.ro Ioana-Cezara CABA cezara.grigoriu@umfiasi.ro Adriana TRIFAN adriana.trifan@umfiasi.ro Silvica PĂDUREANU silvyp27@yahoo.com Luminița AGOROAEI luminita.agoroaei@umfiasi.ro <p style="text-align: justify;">The present review summarizes the literature data regarding the application of <em>Triticum aestivum </em>assay as an alternative method for toxicity assessment of environmental pollutants or potential therapeutic agents. Plant bioassays present several advantages among other biological assays (simplicity, low cost, rapid test activation, a wide array of assessment endpoints). They present a good correlation with animal and human cells models, and are a reliable tool for genotoxicity assessment. Furthermore, in the context of toxicology guidelines that promote the substitution of assays using animal models with other bioassays, genotoxicity assays using higher plants models have gained in popularity. The present review focuses on three major aspects regarding <em>Triticum aestivum </em>assay - its utility in environmental pollution monitoring, its application in genotoxicity assessment studies, and its application in phytotoxicity evaluation of nanomaterials.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-12-04T14:19:30+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11598 Evaluation of the Allelopathic Potential of Leaf Extracts from Dischidia imbricata (Blume) Steud. on the Seedling Growth of Six Test Plants 2019-12-02T14:38:00+00:00 Ramida KRUMSRI ramidakrumsri@gmail.com Sutjaritpan BOONMEE sutjaritpanbm@gmail.com Hisashi KATO-NOGUCHI hisashi@ag.kagawa-u.ac.jp <p style="text-align: justify;"><em>Dischidia imbricata</em> (Blume) Steud. is a herbaceous plant belonging to the Apocynaceae family. This plant has been reported to possess various pharmacological properties, however, there has been no report related to its allelopathic properties. Therefore, the aqueous methanol extracts of <em>D. imbricata</em> were examined for possible allelopathic activity against the seedling growth of dicotyledonous plants; lettuce (<em>Lactuca sativa</em> L.), cress (<em>Lepidium</em><em> sativum</em> L.), and alfalfa (<em>Medicago sativa</em> L.), and monocotyledonous plants; barnyard grass (<em>Echinochloa crus-galli</em> (L.) P. Beauv.), Italian ryegrass (<em>Lolium</em> <em>multiflorum</em> Lam.), and timothy (<em>Phleum pratense</em> L.). <em>D. imbricata</em> extracts exhibited a significant inhibition on the seedling growth of all the test plant species at the concentration ≥ 0.01 g dry weight equivalent extract mL<sup>-1</sup>. The seedling growth of lettuce showed the highest inhibition with <em>D. imbricata</em> extracts followed by alfalfa and cress, whereas the least inhibition was found on the seedling growth of timothy. Concentrations required for 50% inhibition (<em>I</em><sub>50 </sub>values) of all the test plant species&nbsp;were in the range of 0.003 to 0.067 g D.W. equivalent extract mL<sup>-1</sup> for shoot growth, and 0.018 to 0.026 g D.W. equivalent extract mL<sup>-1</sup> for root growth.&nbsp;In addition considering the <em>I</em><sub>50 </sub>values, the root growth of the test plant species was more sensitive to <em>D. imbricata </em>extracts than their shoot growth, except cress seedling. These results indicated that <em>D. imbricata</em> may possess allelopathic activity and may contain allelopathic substances.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-08T16:49:36+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11657 Growth Analysis of Quinoa (Chenopodium quinoa Willd.) in Response to Fertilization and Soil Tillage 2019-11-08T20:22:22+00:00 Ioanna KAKABOUKI i.kakabouki@gmail.com Ioannis E. ROUSSIS iroussis01@gmail.com Panagiota PAPASTYLIANOU yiota.papastylianou@gmail.com Panagiotis KANATAS pakanatas@gmail.com Dimitra HELA dchela@cc.uoi.gr Nikolaos KATSENIOS nkatsenios@gmail.com Francisco FUENTES frfuentesc@uc.cl <p style="text-align: justify;">Growth analysis is an appropriate method for plant response to various environmental and cultural conditions during plant life. A 2-year experiment was conducted to evaluate the effect of soil tillage and fertilization on the growth and growth parameters of quinoa crop and to determine the association between yield and growth characteristics at both the single plant and crop stand level. The experiment was laid out in a split-plot design with two replicates, two main plots [conventional (CT) and minimum tillage (MT)] and four sub-plots [fertilization treatments: untreated, inorganic fertilization of 100 (N1) and 200 kg N ha<sup>-1</sup> (N2) and sheep manure]. The highest absolute growth rate (AGR) and crop growth rate (CGR) values were recorded between the middle of vegetative growth stage and the beginning of anthesis (50-75 DAS) under conventional tillage coupled with manure (AGR: 0.4577 g day<sup>-1</sup>, CGR: 11.44 g m<sup>-2</sup> day<sup>-1</sup>) and with N2 treatment (AGR: 0.4521 g day<sup>-1</sup>, CGR: 11.31 g m<sup>-2</sup> day<sup>-1</sup>). Concerning specific leaf area (SLA), the highest value (150.58 cm<sup>2</sup> g<sup>-1</sup>) was found at 75 DAS in N2 treatment. Leaf area index (LAI) were positively affected by soil tillage and fertilization with greatest values found under conventional tillage coupled with N2 treatment (5.110 m<sup>2</sup> m<sup>-2</sup>). The highest seed yield was observed in N2 treatment (2488 and 2388 kg ha<sup>-1</sup> under CT and MT, respectively). As a conclusion, the cultivation under conventional tillage and the increasing levels of applied nitrogen up to 200 kg N ha<sup>-1 </sup>increases crop growth and yield.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-08T19:57:44+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11633 Drought Avoidance and Phenotypic Flexibility of Sweet Potato (Ipomoea batatas (L.) Lam.) Under Water Scarcity Conditions 2019-11-12T11:50:07+00:00 Carla GOUVEIA csgouveia@staff.uma.pt José F. T. GANANÇA jofitei@staff.uma.pt Humberto G. M. de NÓBREGA humberto.nobrega@staff.uma.pt José G. R. de FREITAS jgrfreitas@staff.uma.pt Vincent LEBOT lebot@vanuatu.com.vu Miguel Â. A. Pinheiro de CARVALHO miguel.carvalho@staff.uma.pt <p style="text-align: justify;">Sweet potato (<em>Ipomoea batatas </em>(L.) Lam.) is an important staple food in several regions of the world. Water scarcity is the most devastating abiotic stress, with a great impact on crop productivity, food security, and subsistence. Drought restricts the nutrient intake and transport into the plant. Tolerant crops have morphological mechanisms of drought avoidance and/or phenotypic flexibility, showing also good water and nutrient efficiency. However, that information is scarce for sweet potato, which is usually based on physiological traits of plant productivity. Here, we show the physiological responses of eight sweet potato accessions subjected to a 3 months’ drought period, by recording their differences for nutrient and leaf chlorophyll content, biomass and stress level. Our results showed that the differences in water use efficiency (WUE, +68.1%), chlorophyll content index (CCI, -5.3%), total plant biomass (TPB, -55.4%), nutrient efficiency (NER, +38.1%) and nutrient harvest index (NHI, +2.9%) where significantly correlated with the water regime. The water shortage led to a drought avoidance response, with TPB loss in all accessions. Distinct phenotypic flexibility responses were also recorded and explained by the root:shoot ratio (R:S) and stress index (SI) variation of the storage root and shoot growth. This information could be relevant for the development of sweet potato breeding programs, adapting this crop to climate change.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-08T17:59:36+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11653 Development of an Efficient ‘One-Step Freezing’ Cryopreservation Protocol for a Georgian Provenance of Chestnut (Castanea sativa Mill.) Zygotic Embryos 2019-11-08T20:23:34+00:00 Mariam GAIDAMASHVILI mariam.gaidamashvili@tsu.ge Eka KHURTSIDZE ekakhurtsidze@tsu.ge Carla BENELLI carla.benelli@cnr.it Maurizio LAMBARDI lambardi@ivalsa.cnr.it <p style="text-align: justify;">Experiments were performed to determine the influence of various dehydration and vitrification treatment times on the ‘one-step freezing’ cryopreservation of embryonic axes (EAs), composed of zygotic embryos and cotyledon residuals, from mature seeds of a Georgian provenance of chestnut (<em>Castanea sativa</em> Mill.). Dehydration was carried out in laminar flow hood from 1 to 5 h, and vitrification experiments were carried out by immersion of EAs in PVS2 vitrification solution up to 120 min, both followed by direct immersion in liquid nitrogen. Both systems resulted in inducing specimen tolerance to ultra-rapid freezing, although to a different extent. Full germination of cryo-stored EAs after 5 h of dehydration (reducing moisture content from initial 66% to 21%) has been increased from 0% to 66.7%. A pre-treatment of EAs in PVS2 vitrification solution for 30 min produced fully developed plantlets at a rate of 55.6% in post-cryopreservation. Plantlet regrowth from cryopreservation was faster in EAs that underwent the dehydration/‘one-step freezing’ procedure. All the plantlet from cryopreserved EAs could be easily acclimatized, producing healthy potted plants. Finally, the TTC test showed to be useful for a fast evaluation of specimen survival after thawing and, as a consequence, to speed up the development of optimized cryo-protocols.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p style="text-align: justify;"><strong>*********</strong></p> 2019-11-08T00:00:00+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/10989 Effect of Nitrogen Fertiliser and Lime on the Floristic Composition, Soil Microbes and Dry Matter Yield of Danthonietum calycinae Grassland 2019-12-02T14:27:04+00:00 Vladimir ZORNIC vladimir.zornic@ikbks.com Vladeta STEVOVIC vladeta@kg.ac.rs Zoran LUGIC zoran.lugic@ikbks.com Snezana ANDJELKOVIC snezana.andjelkovic@ikbks.com Goran JEVTIC goran.jevtic@ikbks.com Jasmina RADOVIC jasmina.radovic@ikbks.com Mirjana PETROVIC mirjana.petrovic@ikbks.com <p style="text-align: justify;">Natural grasslands are significant resources for forage production, which is not exploited enough, mostly because of low production, as a result of bad grasslands management. The research has been carried out on the association <em>Danthonietum calycinae</em> to evaluate the effects of different nitrogen application rates (N<sub>20</sub>, N<sub>80</sub> and N<sub>140</sub>) and lime (1 tha<sup>-1</sup>) on the floristic composition, soil microbes and dry matter yield. Botanic composition was strongly influenced by the treatments, especially by N applications. Nitrogen fertilisation led to significant increase of grass species in the association (from 57% to 82%), while higher rate of nitrogen caused a significant decrease of plants from other families (especially legumes). Application of low and medium rate of nitrogen positively effect on microbial abundant in soil, but the highest dose (140 kg N) reduced all microbial count, except fungi. Mineral nitrogen fertilisation had a favourable effect on dry matter yield in all treatments. The highest increase in dry matter yield relative to the control was in N<sub>140 </sub>treatments (6.66 t ha<sup>-1</sup>). Results indicated that optimal applications of nutrients for this association is application of PK and lime and 80 kg of nitrogen which enable high yield (6.38 t ha<sup>-1</sup>) in comparison with control (3.16 t ha<sup>-1</sup>) and preserving soil fertility and the environment at the same time.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-08T00:00:00+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11527 Effect of Irrigation and Fertilization Levels on Mineral Composition of Cannabis sativa L. Leaves 2019-12-03T09:14:26+00:00 Eleni WOGIATZI wogiatzi@teilar.gr Nikolaos GOUGOULIAS ngougoulias@teilar.gr Kyriakos D. GIANNOULIS kyriakos.giannoulis@gmail.com Christina-Anna KAMVOUKOU cakamvoukou@gmi.com <p style="text-align: justify;">A field experiment was conducted in central Greece to study the effect of two irrigation (I1: 100% ETo, I2: 60% ETo) and N-fertilization levels (N1: 244, N2: 184 kg ha<sup>-1</sup>), on the nutrients concentration of <em>Cannabis sativa</em> leaves (cv. ‘Fibranova’). The&nbsp; N, K, Ca, Mg, P -concentration in the leaves was ranged by 2.8 to 3.51%, 1.8 to 2.57%, 1.96 to 2.17%, 0.86 to 0.88%, and 0.3 to 0.37% respectively, while by the micronutrients the iron showed the highest concentration that ranged by 129 to 139.8 mg kg<sup>-1</sup>dw. The treatment I1F1, where the highest level of irrigation and N -fertilization was applied, compared to the other treatments, showed the highest dry biomass yield, however, in the leaves the highest concentrations of N, K, Mn and Cu were not observed. Moreover, it was found that the N, K, Ca, Mg, P and Fe removal only by one ton dry biomass of leaves was ranged by 28 to 35.12 kg, 18.01 to 25.65 kg, 19.6 to 21.7 kg, 8.34 to 8.75 kg, 3.01 to 3.70 kg and 0.129 to 0.140 kg, respectively. These results could contribute optimal fertilizer application and therefore to the reduction of production costs of the crop.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-08T18:56:32+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11504 Somatic Embryogenesis Induction in Leaf and Root Explants of Allophylus edulis (A.St.-Hil., Cambess. and A. Juss.) Radlk. 2019-11-21T11:47:10+00:00 Thiago da Silva MESSIAS thiagom896@gmail.com Rodrigo Kelson Silva REZENDE rkelson@ufgd.edu.br Luciely Faustino da SILVA lucielyfs@gmail.com Maílson Vieira JESUS mvjagro@gmail.com Geisianny Pereira NUNES geisi.pn@hotmail.com <p style="text-align: justify;"><em>Allophylus edulis </em>(A.St.-Hil., Cambess. and A. Juss.) Radlk., commonly known as cocum, belongs to the Sapindaceae family. This species is of great medicinal interest, with studies showing that its fruits have antioxidant, anti-cholinesterase, and cytotoxic activity. In addition, it is used in traditional medicine as an antidiarrheal, anti-inflammatory and antihypertensive. The objective of this study was to perform somatic embryogenesis <em>in vitro</em> from leaf and root explants of <em>Allophylus edulis</em>, using different 6-benzylaminopurine (BAP) concentrations combined with naphthalene acetic acid (NAA). All treatments exhibited 100% callus formation, except for the treatment without supplementation of growth regulators. The calluses developed in treatments from leaf explants showed up to two colors (brown and brown/cream), and the highest fresh and dry mass was observed in the treatment with 0.5 mg L<sup>-1</sup> of BAP with 0.1 mg L<sup>-1</sup> of NAA. There was no shoot formation from the leaf explants. The callogenesis in treatments from root segments showed callus formation with up to three colors (brown, brown/cream, and cream/green), and the highest fresh and dry mass was obtained when cultivated with 2.0 mg L<sup>-1</sup> of BAP combined with 0.1 mg L<sup>-1</sup> of NAA. These auxin and cytokinin concentrations also showed a higher number of shoots. The interaction between auxin and cytokinin is recommended to obtain somatic embryogenesis in root segments and callus with morphological characteristics suitable for organogenesis.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-12T16:52:37+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11621 Spatial Distribution of Glomalin-related Soil Proteins in Coniferous and Broadleaf mixed Temperate Forest 2019-12-03T09:21:00+00:00 Yongming WANG 1030079720@qq.com Chunhua JI jchlover@163.com Zhaoyong SHI shihaust@163.com Xubin YIN 825779950@qq.com Chenzhou LIU drflying@sina.com <p style="text-align: justify;">Glomalin-related soil protein (GRSP), as an important component of soil organic carbon (SOC) pool, is a glycoprotein produced by the hyphae of arbuscular mycorrhizal fungi (AMF), which play a vital role in carbon and nutrient cycling in forest ecosystem. Here we investigated the spatial distribution of GRSP in plant community of the dominated species not associated with AMF based on a typical coniferous and broad-leaved temperate forest in Mt. Changbai, Northeastern China. Spatial distribution of GRSP including easily extractable GRSP (EEG) and total GRSP (TG) is represented by Moran’s <em>I</em> on different soil depth among seven soil layers of 0-5 cm, 5-10 cm, 10-20 cm, 20-30 cm, 30-50 cm, 50-70 cm and 70-100 cm. The concentrations of EEG and TG decreased with the increase of soil depth according to a logarithmic function. The Moran’s <em>I</em> coefficient of GRSP was negative in all soil layers except TG in 20-30 cm and 50-70 cm soil layers. When EEG and TG were considered, the Moran’s <em>I</em> coefficient was positive in majority of soil layers within the separation distance of less than 4 m but in soil layers of 10-20 cm and 20-30 cm for EEG and in 30-50 cm for TG. The largest Moran’s <em>I</em> coefficient including EEG and TG was observed in the soil layer of 5-10 cm. The spatial distribution of GRSP was discrete in typical coniferous and broad-leaved temperate forest, and was affected by mycorrhizal colonization rate, soil organic carbon and total nitrogen.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-08T00:00:00+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11600 Some Pomological Properties of Promising Seed Propagated Walnut Genotypes from Inner Turkey 2019-11-12T17:00:42+00:00 Aysen KOC aysenkoc@yahoo.com Hakan KELES hakan.keles@bozok.edu.tr Sezai ERCISLI sercisli@hotmail.com <p style="text-align: justify;">In this study, fruits from seed propagated walnut (<em>Juglans regia</em> L.) trees were collected two consecutive years in harvest seasons in Yozgat province in Turkey.&nbsp; Considering two years results, promising five genotypes were determined as cultivar candidate. In the promising genotypes, nut weight ranged from 12.55 (Y11) to 15.08 g (Y15), kernel weight ranged from 5.23 (Y11) to 7.34 g (Y15) and kernel ratio varied between 41.67 (Y11) to 50.84% (Y1), respectively. Linoleic acid was the only polyunsaturated fatty acids and oleic, palmitoleic and gondoic acids determined as major monounsaturated acids ranged from 30.36 to 48.43%, 0.05 to 0.14% and 0.22 to 0.29%, respectively. Propylparaben was the major phenolic acid among the determined phenolic acids in fruits of all five promising genotypes and Y16 had the highest amount of propylparaben (128.08 mg per kg) in its kernel. Malic and tartaric acid were the major organic acids in walnut kernels ranged from 47.88 to 78.51 mg per 100 g and 30.27 to 49.60 mg per 100 g, respectively. L-ascorbic acid was the another organic acids in walnut kernels ranged from 10.71 to 19.71 mg per 100 g. Citric acid was non-determined in kernels of Y1, Y14 and Y15 but determined at kernels of Y11 and Y16 as 4.51 and 7.55 mg per 100 g, respectively. It was determined that the oxalic, malonic, succinic, maleic and fumaric acid contents varied between 8.39-12.08 mg per 100 g, 6.02-9.19 mg per 100 g, 2.86-5.32 mg per 100 g, 0.26-3.00 mg per 100 g and 0.26-0.58 mg per 100 g, respectively.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-12T12:45:32+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11651 Genome-Wide Identification of WRKY Family Genes and Analysis of Their Expression in Response to Abiotic Stress in Ginkgo biloba L. 2019-11-12T17:02:12+00:00 Shuiyuan CHENG s_y_cheng@sina.com Xiaomeng LIU LiuXM925@163.com Yongling LIAO liaoyongling@yeah.net Weiwei ZHANG wwzhangchn@163.com Jiabao YE yejiabao13@163.com Shen RAO raoshen1989@163.com Feng XU xufeng198@126.com <p style="text-align: justify;"><em>Ginkgo biloba</em> is widely planted, and the extracts of leaves contain flavonoids, terpene esters and other medicinal active ingredients. WRKY proteins are a large transcription factor family in plants, which play an important role in the regulation of plant secondary metabolism and development, as well as the response to biotic and abiotic stress. In our study, we identified 40 genes with conserved WRKY motifs in the <em>G. biloba</em> genome and classified into groups I (groups I-N and -C), II (groups IIa, b, c, d, and e), and III, which include 12, 26, and 2 <em>GbWRKY</em> genes, respectively. Meanwhile, the expression patterns of 10 <em>GbWRKY</em> (<em>GbWRKY2</em>,<em> GbWRKY3</em>,<em> GbWRKY5</em>,<em> GbWRKY7</em>,<em> GbWRKY11</em>,<em> GbWRKY15</em>,<em> GbWRKY23</em>,<em> GbWRKY29</em>,<em> GbWRKY31</em>, <em>GbWRKY32</em>) under different tissue and abiotic stress conditions were analyzed. Under stress treatment, the expression patterns of 10 <em>WRKY</em> genes were changed. 10 ginkgo WRKY transcription factors were induced by ETH and SA, but there are two different induced response modes. The expression of 10 <em>WRKY</em> genes was inhibited under low temperature, high temperature and MeJA hormone induction<em>.</em> Most<em> WRKY </em>genes were up-regulated under the induction of high salt and ABA. G<em>bWRKYs</em> were differentially expressed in various tissues after abiotic stress and plant hormone treatments, thereby indicating their possible roles in biological processes and abiotic stress tolerance and adaptation. Our results provided insight into the genome-wide identification of GbWRKYs, as well as their differential responses to stresses and hormones. These data can also be utilized to identify potential molecular targets to confer tolerance to various stresses in <em>G. biloba</em>.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-12T14:37:44+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11520 Intercropping of Faba Bean with Barley at Various Spatial Arrangements Affects Dry Matter and N Yield, Nitrogen Nutrition Index, and Interspecific Competition 2019-11-20T13:17:56+00:00 Christos A. DORDAS chdordas@agro.auth.gr Anastasios S. LITHOURGIDIS lithour@auth.gr Kalliopi GALANOPOULOU chdordas@agro.auth.gr <p style="text-align: justify;">Intercropping is the cultivation of two or more crop species on the same area of land, and can improve yield, forage quality, and soil health. Despite the fact that intercropping is an old practice, it received significant attention the last years because of the environmental impact that it has. However, the effect of the various spatial arrangements of the different species that are used in an intercropping system was not determined. The objective of the present study was to study the yield, growth and nitrogen (N) uptake rate, N nutrition index (NNI) of barley, interspecific competition, quality and financial outcome of intercrops of faba bean (<em>Vicia faba</em> L. var<em>. equina</em>) and barley (<em>Hordeum vulgare </em>L.) with various spatial arrangements (1:1, 2:2, 2:1 alternate rows, and mixed in the same row). The land equivalent ratio (LER), relative crowding coefficient (K), actual yield loss (AYL) and system productivity index (SPI) values were greater for the FB:B intercrop of 2:1, indicating the advantage of intercropping in terms of dry matter and N yield. Sole cropping of barley showed a reduction in NNI by 7 %, whereas NNI for barley increased by an average of 14% in intercropping treatments. Based on biomass production and the competition indices for dry matter and N yield, and NNI the FB:B intercrop of 2:1 was more advantageous than faba bean and barley monocrops, as well as the other intercropping treatments that were tested.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-20T12:16:32+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11522 Foliar Micromorphology and Histochemical Localization of Specific Metabolites in the Leaves of Celosia argentea L. 2019-11-20T13:16:56+00:00 Oluwafunmilayo Dorcas ADEGBAJU funmaj2005@gmail.com Gloria Aderonke OTUNOLA gotunola@ufh.ac.za Anthony Jide AFOLAYAN aafolayan@ufh.ac.za <p style="text-align: justify;"><em>Celosia argentea </em>is an essential vegetable that is cultivated in the western part of Africa. This species has a growing reputation for its role in alleviating nutritional challenges and has found great application as herb of therapeutic importance in many regions of the world. This study investigated the foliar micromorphology and the histochemistry of <em>Celosia argentea</em>. Scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectrometry and staining agents were used to evaluate the micromorphology of the plant. The result revealed sunken amphistomatic stomata distribution on both surfaces of the leaf. Anomocytic and anisocytic stomata complexes were also observed on both epidermal surfaces. A definitive protuberance was observed on the polar end of the adaxial stomata. Stomata apparatus was ellipsoidal with a mean length of 19.60 ± 0.56 µm, while the mean stomata densities on the adaxial and abaxial surfaces were 83.5 ± 2.12 mm<sup>‑2</sup> and 194.18 ± 3.70 mm<sup>‑2</sup> respectively. Trichomes (with a length of 100.94 ± 9.44 µm) were sparsely distributed on both surfaces; glandular trichomes were observed only on the abaxial surface. The EDX spectrometry revealed the presence of iron, oxygen, sodium, calcium, potassium and sulphur as the major elements on the leaf, while histochemical tests revealed the presence of polyphenols, starch and lipids. Our results could be used to support and validate the information database on the anatomy of <em>Celosia argentea</em>.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-20T13:08:30+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11539 Natural Sources of Spraying to Preserve Apple Fruit Quality during Post-Harvest 2019-11-21T07:21:32+00:00 Angelica A. ACEVEDO-BARRERA a.acevedobarrera@gmail.com Juan M. SOTO PARRA jsoto@uach.mx Rosa M. YAÑEZ-MUÑOZ myanez@uach.mx Esteban SANCHEZ esteban@ciad.mx Ramona PEREZ-LEAL rleal@uach.mx <p style="text-align: justify;">One of the great challenges that apple producers face is to substantially improve the quality of fruit, including after harvest. A balanced and timely supply of calcium (Ca) during growth and in the post-harvest stage is considered a technique that could improve shelf life and fruit quality; thus calcium plays a key role during post-harvest, with calcium carbonate and calcium sulphate being an alternative of foliar spraying. The study was carried out on ‘Top Red’ and ‘Golden Delicious’ apple cultivars, in a randomized complete block design with 13 treatments (referring to different commercial presentations of calcium) and 6 repetitions; 8 foliar sprays were applied weekly, from June 22 until harvest; ‘Top Red’ (151 days after full flowering, DDCF) and ‘Golden Delicious’ (145 DDCF) fruit were evaluated in regard with fruit traits (diameter, weight and colour) and maturity (pulp firmness, total soluble solids, titratable acidity and sugar acidity ratio). In ‘Top Red’ cv., calcium carbonate presented the best results, with intermediate to high quality apple fruit, while for cv. ‘Golden Delicious’ the best results were obtained with calcium sulphate, which increased fruits’ diameter, weight, colour and titratable acidity. Even more, of the products evaluated, CaCO<sub>3</sub> and CaSO<sub>4</sub> had the lowest costs, and can be considered as good alternatives of foliar calcium supplement applied in order to preserve the quality of the apple during post-harvest.</p> <p>&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-21T00:00:00+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11474 Phenolic Profiles, Antimicrobial and Cytotoxic Properties of Both Micropropagated and Naturally Growing Plantlets of Calamintha sylvatica subsp. sylvatica Bromf. 2019-12-03T08:58:28+00:00 Mustafa CÜCE mustafacuce@windowslive.com Tuba BEKİRCAN tubabekirca@gmail.com Abdul Hafeez LAGHARI hafeezlaghari58@yahoo.com Münevver SÖKMEN munevver.sokmen@gidatarim.edu.tr Atalay SÖKMEN asokmen61@gmail.com Evren ÖNAY UÇAR evrenonay@istanbul.edu.tr Ali Osman KILIÇ akilic@ktu.edu.tr <p style="text-align: justify;">A rapid micropropagation protocol was designed to produce <em>Calamintha sylvatica</em> plantlets by using nodal segments as explants for the shoot formation. 6-BA favored the highest shoot formation and biomass yield, whilst kinetin was found superior for the highest shoot length (38.97 ± 2.85 mm) and node numbers (2.89 ± 0.63). Rosmarinic acid was detected as major phenolic acid, ranging from 7.59 mg/100 g to 81.44 mg/100 g. Hexane extracts from natural and <em>in vitro</em> propagated plantlets showed activity only against <em>Staphylococcus aureus</em> ATCC 25923 with MIC values at 6.25 and 3.33 m/mL, respectively while in the latter case, extracts from natural plantlets exerted higher cytotoxic activity than those of micropropagated ones (IC<sub>50</sub> values were 83 µg/mL and 98 µg/mL on HeLa cells, respectively). <em>C. sylvatica</em> showed high micropropagation performance and produced remarkable amount of rosmarinic acid <em>in vitro</em> as well as antimicrobial and cytotoxic effect.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-21T10:38:27+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11714 Phytochemical Composition and Antioxidant Activity of Various Grain Amaranth Cultivars 2019-12-10T07:48:23+00:00 Andreea STĂNILĂ andreea.stanila@usamvcluj.ro Bogdan CIOANCA cioancabogdan@gmail.com Zorița DIACONEASA zorita.diaconeasa@gmail.com Sorin STĂNILĂ sorin.stanila@usamvcluj.ro Nicușor SIMA nicusor.sima@usamvcluj.ro Rodica Maria SIMA rodiganea@yahoo.com <p style="text-align: justify;">This study quantified differences in methanolic extracts composition among four grain amaranth cultivars (e.g. ‘Hopy Red Dye’, ‘Amont’, ‘Plenitude’, and ‘Golden Giant’) farmed under three planting conditions: no irrigation/no fertilization (NN), no irrigation/fertilization (NF), irrigation/no fertilization (IN). The study main outcomes were total flavonoids, polyphenols, antioxidant activity, and protein content. Antioxidant activity was assessed using two Single Electron Transfer (SET) based assays: the 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation decolorization assay (ABTS) and cupric reducing antioxidant capacity (CUPRAC). The total protein content was assessed by Gornall spectrophotometric method, the total flavonoid content (TFC) was determined using a colorimetric technique, while total polyphenols content (TPC) was assessed using the Folin-Ciocalteu method. Mean differences in outcomes were calculated using ANOVA and Dunnet’s test for multiple comparisons. The findings revealed that TPC ranged from 5 to 18 mg gallic acid equivalents (GAE)/100 g dry weight (DW), being highest in ‘Plenitude’ under NF conditions. The highest TFC (7.5 mg quercetin equivalent (QE)/100 g DW) and the highest protein content (37.25%) were revealed for the ‘Hopi Red Dye’ cultivar under the NF planting conditions. Amaranth seeds represent a potential rich source of polyphenols and protein gluten-free compounds, with the ‘Hopi Red Dye’ representing the richest cultivar in such compounds. Fertilized and non-irrigated soil provided the optimal planting conditions across all amaranth cultivars.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-12-10T07:40:15+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11561 Mycorrhiza Regulates Signal Substance Levels and Pathogen Defense Gene Expression to Resist Citrus Canker 2019-11-22T13:11:42+00:00 Miao-Miao XIE 972948575@qq.com Yi-Can ZHANG zhangyic1992@163.com Li-Ping LIU llp31302@163.com Ying-Ning ZOU zouyingning@163.com Qiang-Sheng WU wuqiangsh@163.com Kamil KUČA kamil.kuca@uhk.cz <p style="text-align: justify;">Citrus canker is a quarantined disease, severely harming citrus plants. Soil beneficial arbuscular mycorrhizal fungi (AMF) can provide a biological control pathway to resist pathogens. This work was to test changes of signal substances including hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), nitric oxide (NO), calmodulin (CaM), salicylic acid (SA) and jasmonic acid (JA) and the pathogen defense gene expression in roots of AMF (<em>Paraglomus occultum</em>) and non-AMF trifoliate orange (<em>Poncirus trifoliata</em>) seedlings after infected by a expressions citrus canker pathogen (<em>Xanthomonas axonopodis</em> pv. <em>Citri</em>, Xac). AMF inoculation significantly improved plant height, stem diameter and leaf number. Xac infection dramatically decreased root H<sub>2</sub>O<sub>2</sub>, NO, and SA levels, but increased root CaM and JA concentrations in non-AMF seedlings. There were higher H<sub>2</sub>O<sub>2</sub> and CaM levels and lower JA levels in Xac-infected seedlings than in non-Xac-infected seedlings under mycorrhization. Under non-Xac infection, mycorrhizal treatment reduced root H<sub>2</sub>O<sub>2</sub>, NO, and SA but increased CaM and JA levels. However, under Xac infection, mycorrhizal inoculation distinctly accelerated root H<sub>2</sub>O<sub>2</sub>, NO, CaM, and SA accumulation, accompanied with up-regulated expression levels of root <em>PtEPS1</em> (enhance pseudomonas susceptibility 1) and <em>PtPR4</em> (pathogenesis related gene 4), indicating that Xac stimulated mycorrhizal roles in enhancing resistance of citrus canker. Such results imply that citrus plants with pre-inoculated AMF had stronger resistance to Xac infection through increasing signal substrate accumulation and pathogen defense gene expressions.</p> <p>&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-22T13:09:39+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11610 Identification and Characterization of a SEPALLATA-like MADS-box Gene from Cucumber (Cucumis sativus L.) 2019-11-22T15:36:43+00:00 Yanwei CHENG chengyanwei@lynu.edu.cn Peng HE hepeng_17@163.com Lunwei JIANG jlp_1999@163.com Shiqiang LIU lsq_hn306@163.com Yong ZHOU yzhoujxau@163.com <p style="text-align: justify;"><em>SEPALLATA</em> (<em>SEP</em>) genes encode the E class MADS-box transcription factors that play vital roles in various aspects of plant growth and development. In this study, we isolated a <em>SEP</em>-like gene from cucumber (<em>Cucumis sativus</em> L.), which was previously named as <em>CsMADS01</em>. <em>CsMADS01</em> had a coding sequence (CDS) of 741 bp, and coded a deduced protein of 246 amino acid residues that was predicted to be located in the nucleus. The putative CsMADS01 protein was typically characterized by the MIKC-type MADS (including MADS, I, K, and C domains) and shared high homology with other SEP-like proteins. Phylogenetic analysis of CsMADS01 and SEP proteins from other plants revealed that CsMADS01 was a member of the SEP1/2 clade of SEP proteins and was the most closely related to ZjMADS48 from Chinese jujube. Promoter analysis showed that several <em>cis</em>-elements related to stress response and hormones were present in the promoter region of <em>CsMADS01</em>. Expression analysis indicated that <em>CsMADS01</em> was highly expressed during the development of male and female flowers, and the expression increased gradually along with fruit development. Ectopic expression of <em>CsMADS01 </em>in <em>Arabidopsis</em> resulted in a phyllody-like phenotype and the transgenic plants never flowered. These results suggest that <em>CsMADS01</em> plays an important role in the growth and development of cucumber.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-22T15:36:42+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11613 Feasibility Study on Reducing Lead and Cadmium Absorption by Alfalfa (Medicago scutellata L.) in a Contaminated Soil Using Nano-Activated Carbon and Natural Based Nano-Zeolite 2019-11-28T13:35:54+00:00 Tahereh HASANABADI t.hasanabadi@gmail.com Shahram LACK sh.lack@yahoo.com Adel MODHEJ adelmodhej2006@yahoo.com Hossien GHAFOURIAN ghaforian25@yahoo.com Mojtaba ALAVIFAZEL mojtaba_alavifazel@yahoo.com M. Reza ARDAKANI mreza.ardakani@gmail.com <p style="text-align: justify;">The first risk posed by heavy metal pollution in an ecosystem is metal accumulation in the biomass of growing plants, which has harmful effects on human health. Natural-based nanoparticles are efficient in remediating environmental pollutants because they have a high surface/volume ratio, high chemical activity and produce no harmful side-products. The present study investigates the capacity of natural-based nano-porous adsorbents for reducing the availability of heavy metals to annual alfalfa (<em>Medicago scutellata</em> L.) roots and keeps them in soil. In a factorial experiment based on a randomized design (with four replications), three nano-adsorbents (nano-activated carbon, natural nano-zeolite and modified nano-zeolite) and two heavy metals (lead and cadmium) have been tested. The results demonstrated that applying the highest rate of activated carbon and modified nano-zeolite reduced shoot Pb content by 34% and 33.2%, and shoot Cd content by 35.5% and 46.7%, respectively, compared with the adsorbent-free control.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-28T11:53:30+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11577 Bagging Affecting Sugar and Anthocyanin Metabolism in the Ripening Period of Grape Berries 2019-11-28T08:05:58+00:00 Qian ZHA zhaqian1988@163.com Xiao Jun XI xxj220401@126.com Yani HE hayani1113@163.com Ai Li JIANG putaojal@163.com <p style="text-align: justify;">Grapevine is one of the most important fruit-bearing plants worldwide, for which bagging treatments can effectively improve fruit quality. However, the low-light conditions caused by bagging can delay grape berry maturation. Here, we analyzed glucose, fructose, and anthocyanin contents and the expression of sugar and anthocyanin-metabolism pathway genes in the grape berries of two cultivars, ‘Shenhua’ and ‘Shenfeng’, under different bagging treatments. Color development was incomplete in bagged grape berries and their soluble sugar contents were lower than those detected in un-bagged fruits. However, fruit color and SSC could be rapidly restored to normal levels after removing bags. Light affects the accumulation of sugar in grape berries, especially near the maturation period, as well as the contents and compositions of anthocyanins in the skin of grape berries. Although light helps in the accumulation of anthocyanins, significant differences were detected in anthocyanin composition between the two grapevine varieties. In addition, the expressions of myofibroblastic regulatory genes in the anthocyanin pathway were affected by light, and the light-responsive elements elongated hypocotyl 5 and constitutive photomorphogenic 1 acted synergistically to control grape berry coloration. Overall, these results provide a theoretical basis for the maturation mechanism in grape berries.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-28T08:01:13+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11712 Cotton Response to Planting Patterns under Effect of Typical and Limited Irrigation Regime 2019-12-06T18:46:15+00:00 Mohammed K. DARAWSHEH ethiage@otenet.gr Ioanna KAKABOUKI i.kakabouki@gmail.com Ioannis ROUSSIS iroussis01@gmail.com Dimitrios J. BILALIS bilalis@aua.gr <p style="text-align: justify;">Decrease of width between cotton (<em>Gossypium hirsutum</em> L.) row spacing has been suggested as an agronomic practice that may increase the yield and reduce the cost production. Altering agronomic practices as row spacing system as well as irrigation regime can affect cotton growth parameters, yield components, and physical fiber properties. The object of this study is the assessment of interaction between row spacing cropping systems and irrigation regime and their effects on yield components. Yield, biomass, harvest index, seed index, seed weight, bolls number, individual boll weight, the proportion of lint and seed were studied in three row spacing systems [conventional row (CR), narrow row (NR) and ultra-narrow row (UNR)] under typical and limited irrigation regime, during two growing seasons. The decrease of row spacing had different effect on yield components under the typical and limited irrigation regime e.g. the differences between systems of row spacing on bolls number were more evident under the limited irrigation than the typical one, and the opposite was true for the lint proportion. Decreased row spacing had positive effects on yield, biomass, bolls number per land area and lint proportion in two irrigation systems. However, had negative impact on individual boll weight, seed weight, seed index, harvest index and seed proportion. Interaction of row spacing and irrigation regime was significant for seed index. High differences were more significant between UNR and CR and less between NR and CR. UNR system appeared to be viable alternative to traditional row system for cotton production.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-28T09:47:26+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11567 Carbon-Nitrogen Metabolic Responses and Adaptive Strategies to Low-Nitrogen Stress in Glycine soja 2019-11-28T09:31:22+00:00 He WANG 1822416631@qq.com Rui GUO guor219@yahoo.com Yongjun HU huyongjun68@sina.com Defu HAN handf67@163.com <p style="text-align: justify;">Nitrogen (N) is an essential mineral nutrient for plant growth and development. Wild soybean (<em>Glycine soja</em>), which has many superior traits, is an important germplasm resource and is also an excellent experimental material for researching the mechanisms of low-N tolerance. In this study, the physiological differences between common wild soybean (W1) and low-N tolerant wild soybean (W2) among growth characteristics, photosynthetic carbon (C) metabolism, N metabolism and C-N metabolic-coupling relationship were investigated, and the mechanism of low-N tolerance of wild soybean was explained at three different levels of low-N stress. Both W1 and W2 showed some resistance to low-level N stress. However, W2 could withstand the damage by increasing the root length and root–shoot ratio under high-level stress conditions. Moreover, when resisting low-N stress, W2 maintained a stable photosynthetic rate and coordinated ion balance to maintain required nutrient levels. W2 also tolerated low N by coordinating the C-N metabolic balance through the accumulation of soluble sugars to provide energy and C skeletons for N metabolism and through enhanced N metabolic enzyme activities and soluble protein accumulation levels to supply the enzyme proteins and photosynthetic pigments for C metabolism. The current results provide a physiological methodology and theoretical basis for protecting wild soybean germplasm resources and improving cultivated soybean.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-28T09:26:09+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11608 Study on Leaf Epidermis Structure and Dust-Retention Ability of Five Machilus Species 2019-11-28T15:24:39+00:00 Xinru HE hexinru10@163.com Yumu ZHANG zhangyumu05@163.com Bing SUN 1902113155@qq.com Pujie WEI 1902113155@qq.com Die HU 1293446583@qq.com <p style="text-align: justify;">Taking the leaves of five <em>Machilus</em> species as a research object, they are <em>Machilus oreophila</em>, <em>Machilus chinensis</em>, <em>Machilus microcarpa</em>, <em>Machilus lichuanensis </em>and <em>Machilus suaveolens</em>. By measuring leaf surface area, cell length and width , the total dust retention, pH value of retention fluid and the content of Pb, Cr, Fe, Cu and Cd of metallic elements were measured, and compare the dust retention of five plant seedling leaves ability. The results showed that: the leaves of different kinds of <em>Machilus</em> have different dust abilities. Comprehensive analysis can be obtained from five kinds of <em>M</em>achilus dust ability in descending order of <em>M. suaveolens </em>&gt; <em>M. lichuanensis </em>&gt; <em>M. chinensis </em>&gt; <em>M. microcarpa </em>&gt; <em>M. oreophila.</em></p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-28T15:22:09+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11614 Roles of Some Plant Growth Regulators on Natural and Dark-Induced Senescence in Wheat Leaf Segments 2019-11-28T14:38:53+00:00 Nihal GÖREN-SAĞLAM gorenn@istanbul.edu.tr Fazilet Özlem ÇEKIÇ faziletozlem@gmail.com <p style="text-align: justify;">Senescence is an important developmental process in the life span of a plant. It is affected by several endogenous and exogenous factors such as hormones, darkness, and drought. Hormones are major endogenous factors that affect senescence intensively. In this study, our aim was to investigate the effects of different plant growth regulators; benzyl adenin (BA), spermine (Spm) and spermidine (Spd) on both natural and dark-induced senescence and on γ-aminobutyric acid (GABA) content in wheat leaf segments. Following the incubation, fresh and dry weight, chlorophyll and carotenoid contents; peroxidase activity, soluble protein, GABA and malondialdehyde (MDA) contents and cell death amount were determined. Our results demonstrate that Spm and Spd accelerated senescence under light and delayed it under dark conditions. BA has delayed leaf senescence under both conditions. Cell death and MDA content showed a correlation with the senescence process. GABA content was enhanced considerably by BA, Spm and Spd applications under light conditions. According to our results we can conclude that Spm and Spd could promote senescence on wheat segments under light conditions, whereas inhibit senescence under dark conditions. In addition, BA could retard the deleterious effects on senescence under both light and dark conditions.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-28T00:00:00+00:00 Copyright (c) https://notulaebotanicae.ro/index.php/nbha/article/view/11641 Effects of Mycorrhiza Inoculation and Grafting for Sweet Pepper (Capsicum annuum L.) Crop Under Low-Tech Greenhouse Conditions 2019-11-28T14:03:48+00:00 Attila OMBÓDI ombodi.attila@mkk.szie.hu Andrea CSORBAINÉ GÓGÁN gogan.andrea@mkk.szie.hu Zita BIRKÁS birkas.zita@kertk.szie.hu Noémi KAPPEL kappel.noemi@kertk.szie.hu Claudio Kendi MORIKAWA ckm@affrc.go.jp Noémi KOCZKA koczka.noemi@mkk.szie.hu Katalin POSTA posta.katalin@mkk.szie.hu <p style="text-align: justify;">In low-cost, unheated greenhouses and tunnels the use of arbuscular mycorrhizal fungi (AMF) and/or grafting can be a less expensive and sustainable solution to combat the adverse effects of monoculture, instead of costly soilless culture. The aim of the present study was to investigate the effects of a commercially available AMF inoculant and grafting on sweet pepper, under circumstances of modelling commercial low-tech greenhouse production. ‘SV9702PP F1’ sweet pepper hybrid was cultivated for seven months in an unheated greenhouse. Beside the control, three treatments were applied: ungrafted AMF treated plants, plants grafted on ‘Bagi F1’ hybrid and AMF treated plus grafted plants. AMF was applied into the planting holes just before transplanting. AMF treatment had positive effects on relative chlorophyll content of leaves (expressed in SPAD value), on plant stand, on plant mass production, on yield and on root colonization rate, despite the high presence of indigenous populations of AMF in the greenhouse soil. With the applied rootstock/scion combination, grafting did not significantly affect the aforementioned parameters. SPAD values were increased by the AMF treatment during periods when smaller doses of nitrogen (less than 0.8 g N per m<sup>-2</sup> week<sup>-1</sup>) were applied. Significant positive correlation was found between root colonization rate and marketable yield. AMF treatment increased the yield by 18% (from 12.43 to 14.74 kg m<sup>-2</sup>), mostly due to higher number of fruits. Yield increase was mainly realised during the last third of the harvest period, when the applied nutrient doses were low and temperature conditions were suboptimal.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-28T13:58:53+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11637 Effects of Shading on Carbohydrates of Syzygium samarangense 2019-11-30T08:47:17+00:00 Ying JIAN 491257864@qq.com Guolin WU 1293831688@qq.com Donghui ZHOU 910441075@qq.com Zhiqun HU zhqhu@scau.edu.cn Zhenxuan QUAN 185191743@qq.com Biyan ZHOU zhoubiyan@scau.edu.cn <p style="text-align: justify;">Wax apple (<em>Syzygium samarangense</em>) is an important tropical fruit tree cultivated in Southeast Asian. It produces red pear-like shape fruits. The fruit flesh is considered high in antioxidants, phenolics, and flavonoids that have a potential to contribute to the human healthy diet, and was proved to have anti-inflammatory and antimicrobial characteristics. To allow year-round marketing of high quality wax apple fruit, growers always perform shading to inhibit new flushes so as to repress vegetative growth and promote reproductive growth. To investigate the effect of shading on carbohydrates, wax apple trees were shaded with sun shade nets under field conditions. The effects of shading on shoot growth were studied and leaf carbohydrate levels of the trees were determined. The results showed that shading inhibit the the growth of the terminal shoots and promoted bud dormancy. Shading also reduced total soluble sugar, sucrose, glucose, fructose, and starch levels of leaves. The results suggested that shading reduced carbohydrate accumulation and repressed vegetative growth.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-29T11:52:55+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11672 The Estimation of Nitrogen Uptake and Utilization Efficiency in Cotton by the Fertilizer-Response Model 2019-11-30T07:01:11+00:00 Lulu MA 1849819772@qq.com Qiang ZHANG swshzulzst@foxmail.com Jiao LIN lxshz@126.com Wei SU swshzulzst@foxmail.com Caixia YIN zhangze1227@shzu.edu.cn Xin LV lxshz@126.com Ze ZHANG zhangze1227@shzu.edu.cn <p style="text-align: justify;">Due to the indeterminate growth habit of cotton crops, a better understanding of N status at the rational fertilizer regime is important to promote lint yield. The fertilizer-response model was employed to evaluate N status by analyzing data of shoot dry mass, N content and N concentration at different growing stages. A field study was conducted on drip-irrigated cotton plants with N fertilizer addition in total amounts of 0 (N0), 120 (N1), 240 (N2), 360 (N3) and 480 (N4) kg ha<sup>-1</sup> in Xinjiang, China in 2016. Thirty percent of total fertilizers were applied at planting and the rest 70% were applied over six applications. The N fertilizer treatment at the accumulative rate of 70 kg ha<sup>-1</sup> was enough to induce the N status of steady state accumulation 60 days after germination. Since 90 days the treatments that delivered the N amount between 120 and 240 kg ha<sup>-1</sup> was deficient for cotton demand, higher rates from 360 and 480 kg ha<sup>-1</sup> induced inherent N reserve and resulted in the highest level of yield. With regard to the practical meaning, the N fertilizer dose of 360 kg ha<sup>-1</sup> can be used for cotton growth. The N fertilizer dose of 120 kg ha<sup>-1</sup> can be recommended when the yield of 5,840 kg ha<sup>-1</sup> lint can meet the goal of cotton culture.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-30T06:57:18+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11216 Determination of Blooming, Pollen and Fruit Set Characteristics in Punica granatum 2019-11-30T07:33:08+00:00 Deniz AKSOY denizaksoy75@gmail.com Zeynel DALKILIC zdalkilic@adu.edu.tr <p style="text-align: justify;">The objective of this study was to investigate flowering, pollen, and fruit set characteristics of locally registered pomegranate cultivars between two consecutive years. ‘Dr. Ercan 35’, ‘Efenar 35’, ‘Kamilbey 35’, and ‘Tezeren 35’ cultivars have been developed by cross-breeding programs. Open-, cross-, and self-pollination studies were performed with these cultivars using three trees in each replicate in the field. Number of anthers per flower, number of pollen grains per anther, viability, and germination tests were conducted in the laboratory. Fruit set ratio of self-pollinated combinations were lower than open-pollination. The fruit set ratio from self-pollination was obtained from ‘Dr. Ercan’ (60.3%), ‘Efenar 35’ (62.4%), ‘Kamilbey 35’ (50.5%), and ‘Tezeren 35’ (25.2%). The highest fruit set ratio (49.7%) was obtained from ‘Kamilbey 35’ × ‘Dr. Ercan 35’ combination. The highest number of characteristics was as follows: ‘Tezeren 35’ (402 anthers/hermaphrodite flowers), ‘Kamilbey 35’ (8550 pollen/anther, male flowers in the beginning of flowering), ‘Efenar 35’ (84.7% pollen viability, hermaphrodite flowers), ‘Kamilbey 35’ (71.2%, pollen germination, hermaphrodite flowers). In conclusion, at least one, two or more, pollinator cultivars are necessary for commercial plantations using with these recently released pomegranate cultivars.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-30T07:27:37+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11460 Evolution of the Polyphenols, Flavonoids, and Tannins Content in Walnut Leaves and Green Walnut Husk during Growing Season 2019-11-30T09:23:02+00:00 Simona GIURA simona_vam01@yahoo.com Mihai BOTU btmihai2@yahoo.com Mădălina VULPE tudorradumadalina@yahoo.com Loredana Elena VÎJAN vloredana2005@yahoo.com Rodi MITREA rodimitrea@yahoo.com <p style="text-align: justify;">The walnut leaves and the green walnut husk are been widely used in folk medicine. These vegetal materials have been reported as a source of bioactive molecules such as the phenolic compounds. In this research, there were analysed different quality parameters afferent to several walnut varieties, harvested from the germplasm collection located at S.C.D.P. Vâlcea, Romania. Romanian cultivars ‘Jupâneşti’, ‘Valrex’ and ‘Valmit’ and French one ‘Franquette’ were analyzed. The variation in bioactive compounds content from the walnut leaves and walnut husk at different developmental stages (from June to September) was investigated. The results showed that the content in polyphenols, flavonoids and tannins has significantly increased until the ripening stage, after that a decrease of the values of these parameters follows. Greater values of the three parameters were also observed at walnut leaves and green walnut husk of cultivars attacked by walnut blight, caused by the bacterium <em>Xanthomonas arboricola</em> pv. <em>juglandis (Xaj)</em>. The findings of this study reveal the mode of variation in the content of polyphenols, flavonoids and tannins during the growing season of the walnut leaves and green walnut husk, emphasizing also the influence of <em>Xaj</em>. The results obtained demonstrated the potential of the walnut leaves and the green walnut husk as an economical source of antioxidant and antimicrobial agents.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-11-30T09:17:21+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11602 Effect of Sunny and Half-shaded Sites on the Growth of Plants and Anthocyanin Pigments Content in Leaves of Heuchera L. ‘Mysteria’ and ‘Peppermint Spice’ Cultivars 2019-12-05T14:13:12+00:00 Beata JANOWSKA beataj@up.poznan.pl Piotr K. CZUCHAJ piotr.czuchaj@up.poznan.pl Jan KUBIŚ jan.kubis@up.poznan.pl Magdalena RYBUS-ZAJĄC magrybus@up.poznan.pl Daniela KOKOCIŃSKA daniela.karasiak@gmail.com <p style="text-align: justify;">Two cultivars of <em>Heuchera </em>L. - ‘Mysteria’ and ‘Peppermint Spice’ were grown in a collection at a sunny site and a slightly shaded one. The access of sunlight was limited by shade nets, which were stretched on wooden scaffolding over the plants. The dynamics of growth of the plants in the sunny and half-shaded sites was assessed in the growth periods, in two consecutive years. After the second year, the content of anthocyanins in the leaves of different physiological maturity (young, mature and fully mature) was measured. Apart from August, during the whole growth period both cultivars formed more leaves during the first year of cultivation. Both the ‘Mysteria’ and ‘Peppermint Spice’ cultivars formed more leaves at the sunny site. The research showed that the highest content of anthocyanins in the<em> Heuchera </em>‘Mysteria’ and ‘Peppermint Spice’ cultivars growing at the sunny and half-shaded sites was found in young leaves, whereas the lowest content was noted in fully mature leaves. The highest content of anthocyanins in both<em> Heuchera </em>cultivars was found at the initial growth period, i.e. between May and July, when the light intensity was the greatest, regardless of the place of cultivation. At the end of the growth period, the biosynthesis of anthocyanins decreased significantly. Anthocyanins accumulated in the vacuole are able to play a role as the screening pigments under intensive PAR and UV-B radiation.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-12-04T07:31:06+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11718 Influence of Volatile Peppermint (Mentha ×piperita L.) Compounds on Germination and Seedling of Radish (Raphanus sativus L. var. radicula Pers.) Growth 2019-12-05T06:50:03+00:00 Renata SZAFRANIEC renata.purzycka@op.pl Katarzyna MOŻDŻEŃ kasiamozdzen@interia.pl Beata BARABASZ-KRASNY beata.barabasz-krasny@up.krakow.pl Peiman ZANDI z_rice_b@yahoo.com Yaosheng WANG wangyaosheng@caas.cn <p style="text-align: justify;">The research concerns the influence of volatile compounds contained in dry peppermint leaves (<em>Mentha ×piperita</em> L.) on germination and seedling characteristics of radish (<em>Raphanus sativus</em> L. var. <em>radicula</em> Pers. ‘Półdługa’). 1, 5 and 10 g of dried peppermint leaves were added respectively into containers, and then Petri dishes with radish seeds were placed in each of them. The control was performed without dried mint. Radish seeds treated with volatile mint compounds in the lowest 1g dose germinated similarly to the control sample. The other two doses, 5 and 10 g, inhibited the process of seeds germination. Allelophatins from <em>M. ×piperita</em> at 1 g of dose had also a stimulating effect on the growth of radish seedlings, especially its roots. The growth of underground organs was also observed at 5 and 10 g doses. For radishes grown in the lowest dose of allelopathic compounds, the values of fresh and dry masses and percentage water content were highest. As the dose of emitters increased, a slight decrease in the value of these parameters was observed, as compared to the control.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> <p>&nbsp;</p> 2019-12-05T06:37:08+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11604 Morpho-physiological Characteristics of Basil (Ocimum basilicum L.) under NaCl-stress and Rhizophagus fasciculatum as NaCl-stress Mitigator 2019-12-05T11:00:10+00:00 Yuneisy Milagro AGÜERO-FERNÁNDEZ yaguero81@gmail.com Luis Guillermo HERNÁNDEZ-MONTIEL lhernandez@cibnor.mx Bernardo MURILLO-AMADOR bmurillo04@cibnor.mx José Manuel MAZÓN-SUÁSTEGUI jmazon04@cibnor.mx Carlos Michel OJEDA-SILVERA cojedas1979@gmail.com Daulemys BATISTA-SÁNCHEZ daule83@gmail.com <p style="text-align: justify;">Salinity stress is one of the main problems limiting growth and development of cultivated species. The objective of this study was to assess NaCl-stress basil plants (<em>Ocimum basilicum </em>L.) cv. ‘Nufar’ and to determine whether the mitigating effect of an arbuscular mycorrhizal fungus strain (AMF). A completely randomized factorial design was used considering three NaCl concentration (0, 50 and 100 mM) as factor 1 and presence or absence of AMF (0 and 10 g of inoculum) as factor 2, with four replicates per treatment and four plants per repetition. The assessed response variables were, fresh and dry of aerial part and root, root length, leaf area, relative water content, water potential, plant height, number of spores and mycorrhizal colonization percentage after 20 and 50 days (T<sub>20</sub>, T<sub>50</sub>) of the experiment. The results showed greater values in all variables in the control group (0 mM NaCl) than in plants inoculated with <em>R. fasciculatum </em>with T<sub>20</sub> and T<sub>50</sub>; although values decreased as NaCl concentration increased; the tendency to increase was maintained even in at 50 and 100 mM of NaCl with AMF with respect to 50 and 100 mM NaCl without AMF. The AMF colonization percentage decreased as NaCl concentration increased. Nonetheless, the development and growth response for all variables in the inoculated plants with AMF was greater <em>vs</em> non-inoculated, which suggests that basil plant inoculation with AMF has a positive effect in mitigating NaCl stress.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-12-05T07:52:00+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11664 Genetic Diversity of some Daffodil (Narcissus L. spp.) Genotypes in Turkey by Using SRAP Markers 2019-12-05T16:02:54+00:00 Emrah ZEYBEKOĞLU zeybekoglu@gmail.com Tansal KAYGISIZ ASCIOGUL tanselkaygisiz@gmail.com Hulya ILBI hulyailbi@gmail.com Mustafa E. OZZAMBAK m.ercan.ozzambak@ege.edu.tr <p style="text-align: justify;">Daffodil (<em>Narcissus</em> L. spp.) is one of the oldest ornamental flowers that have been cultivated from ancient times to nowadays. The species <em>Narcissus tazetta</em> L. and <em>N. serotinus</em> L. have spread out in different regions of Turkey and some other species have been naturalized. The purpose of this study was to characterize some daffodil populations that were collected from different regions of Turkey by using SRAP markers. Twenty-two daffodil populations of different species such as <em>N. tazetta</em>, <em>N. serotinus</em>, <em>N. pseudonarcissus</em> L., <em>N. poeticus</em> L. and <em>N. jonquilla</em> L. were used. Thirty-seven of 48 SRAP primer combinations showed scorable and polymorphic bands between samples. The similarity coefficients were used to construct a dendrogram by Unweighted Pair-Group Method with Arithmetic Averages (UPGMA) using NTSYS-PC. Cophenetic correlation between ultrametric similarities of tree and similarity matrix were high (r = 0.97). The genotypes of <em>Narcissus</em> species were divided into 2 main groups. Although there was less genetic variation among <em>N. tazetta</em> species, even collected from different regions of Turkey, <em>N. poeticus</em>, <em>N. pseudonarsis</em>, <em>N. jonquilla</em> and <em>N. serotinus</em> had more genetic divergence among them. It can be concluded that genetic variation of <em>Narcissus</em> species can be used for daffodil breeding programs.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-12-05T15:03:22+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11624 Favourable and Restrictive Factors for Quercus pubescens in the Transylvanian Basin, Evaluated by GIS Techniques 2019-12-10T11:15:21+00:00 Vasile ȘIMONCA simoncavasile@gmail.com Sanda ROȘCA sanda.rosca@ubbcluj.ro Alexandru COLIȘAR alexandrucolisar@gmail.com Florin REBREAN florinrebrean88@gmail.com Ștefan BILAȘCO sbilasco@geografie.ubbcluj.ro <p style="text-align: justify;">A very simple analysis of the forested areas across the Transylvanian Basin shows that they cover approximately 45.8% of the area, according to data provided by the European Environment Agency. In order to extend the areas covered by forests, especially over badlands specific for the Transylvanian Basin and to increase the economic and environmental value of these lands, a GIS model of spatial analysis has been developed to identify the areas favourable for downy oak (<em>Quercus pubescens</em>) plantations, a forest species which has specific requirements in terms of adaptability conditions. The developed spatial analysis model is based on the unitary analysis of the climatic, soil and geomorphologic components, spatially materialized as raster format databases, and their integration according to spatial analysis equations in order to get a modelled database which represents spatially the favourable areas for the creation of downy oak plantations. The result of this study highlights the territories which provide favourable but also restrictive conditions for <em>Quercus pubescens</em>. The model has a high predictability rate taking as comparative reference the direct monitoring at the level of forest planning units (PUs) within Cluj County, identified from the analysis of forestry plans. The high validation rate of the proposed model was obtained by overlapping the favourability classes which themselves were obtained after modelling with the limits of the forest planning units where the consistency of the downy oak is greater than 0.7. According to the presented validation procedure, a 93% validation rate was obtained, fact which highlights the usefulness of applying the model in areas having similar features and its extrapolation in areas where the environmental conditions present only slight differences.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First.&nbsp;</strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-12-06T18:13:48+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11696 Assessment of Genetic Diversity of an Endangered Species Fraxinus hupehensis Based on ISSR Markers 2019-12-10T08:34:58+00:00 Peng-Li ZHENG 1271379364@qq.com Jian-Ru CHENG 553894234@qq.com Long-Qing CHEN chenlq0206@163.com Ming-Qin ZHOU 500548@yangtzeu.edu.cn <p style="text-align: justify;">Investigation on the level and pattern of genetic diversity of 10 natural populations of the endangered species <em>Fraxinus hupehensis</em> using inter-simple sequence repeat (ISSR) markers was crucial for understanding the structure of the population and assessing the best genetic protection strategies. A total of 180 polymorphic bands with the polymorphic rate of 100.00% were amplified by 14 primers. The genetic diversity at population level (Percentage of polymorphic loci, PPL=64.06; Nei’s gene diversity index, <em>h</em>=0.1519; Shannon’s information index, <em>I</em>=0.2434) was lower than that at species level (PPL= 100.00%, <em>h</em>=0.1833, <em>I</em>=0.3041). Analysis of molecular variance (AMOVA) demonstrated the low level of the genetic variation occurred between the populations (16.05%). This also can be corroborated by the gene flow (Nm 2.424) and the coefficient of gene differentiation (<em>G</em>st=0.1710) among populations. Cluster analysis based on the unweighted pair group method with arithmetic averages (UPGMA) revealed four groups for 10 populations according to Nei’s genetic identity and seven categories for the 196 individuals according to SM values. Furthermore, the endangered mechanism and genetic structure of <em>F. hupehensis</em> were discussed, and appropriate targeted protection measures were proposed based on these findings.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-12-10T08:16:16+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://notulaebotanicae.ro/index.php/nbha/article/view/11687 Inhibitory Effects of Iso-α and β Hop Acids Against Pediococcus pentosaceus 2019-12-10T10:52:27+00:00 Delia MICHIU delia.michiu@usamvcluj.ro Frank DELVIGNE f.delvigne@ulg.ac.be Nicolas MABON n.mabon@cra.wallonie.be Mirela JIMBOREAN mirela.jimborean@usamvcluj.ro Melinda FOGARASI melinda.nagy@usamvcluj.ro Mihaela MIHAI mihaela.mihai@usamvcluj.ro Maria TOFANĂ maria.tofana@usamvcluj.ro Philippe THONART p.thonart@ulg.ac.be <p style="text-align: justify;">The goal of the research was to assess the inhibitory effects of&nbsp; hop extracts, iso-α and β acids, against <em>Pediococcus pentosaceus</em> bacteria, during a short incubation period, both in liquid selective media (high pH values) and beer wort fermentation (low pH values) and testing if the identified iso-α acid stress changes the activity of <em>S. cerevisiae boulardii</em> yeast and ethanol production. Flow cytometry analysis was used for bacterial and yeast cell viability. In relation to the antibacterial activity of β-acids, a lower viability of <em>Pediococcus pentosaceus</em> cells was observed after a short incubation period in selective media, under iso-α acid stress. In beer wort, for a mixed culture with<em> P. pentosaceus </em>bacteria and<em> S. cerevisiae boulardii</em> yeast, under iso-α acid stress conditions at pH 4.0-5.0, <em>Pediococcus pentosaceus</em> exhibited lower cell viability (20.7%) than in selective media (61.4%). Regarding iso-α hop acid on <em>S. cerevisiae boulardii</em> yeast, the results showed that iso-α does not change the <em>S. cerevisiae</em> activity but prevents the culture from being contaminated by <em>Pediococcus pentosaceus. </em>The results highlighted reliable inhibitory effects of iso-α and β-acids against <em>P. pentosaceus</em>, both at pH 6.0-7.0 and pH 4.0-5.0, which open the possibility of hops being used as a supplement to prevent beverage contamination with spoilage microorganisms.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 4, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> 2019-12-10T10:49:26+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca