TY - JOUR AU - FAN, Sun AU - AHMAD, Naveed AU - LIBO, Jin AU - XINYUE, Zhang AU - XINTONG, Ma AU - HOANG, Nguyen Q. V. AU - MALLANO, Ali I. AU - NAN, Wang AU - ZHUODA, Yang AU - XIUMING, Liu AU - NA, Yao PY - 2021/09/27 Y2 - 2024/03/28 TI - Genome-wide investigation of Hydroxycinnamoyl CoA: Shikimate Hydroxycinnamoyl Transferase (HCT) gene family in Carthamus tinctorius L. JF - Notulae Botanicae Horti Agrobotanici Cluj-Napoca JA - Not Bot Horti Agrobo VL - 49 IS - 3 SE - Research Articles DO - 10.15835/nbha49312489 UR - https://notulaebotanicae.ro/index.php/nbha/article/view/12489 SP - 12489 AB - <p>Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) is mainly associated with monolignol biosynthesis, a central precursor to producing guaiacyl and syringyl lignins in plants. However, the explicit regulatory mechanism of HCT-mediated monolignol biosynthesis in plants still remained unclear. Here, the genome-wide analysis of the <em>HCT</em> gene family in <em>Carthamus tinctorius</em> as a target for understanding growth, development, and stress-responsive mechanisms was investigated. A total of 82 <em>CtHCT</em> genes were identified and characterized. Most of the CtHCTs proteins demonstrated the presence of two common conserved domains, including HXXXD and DFGWG. In addition, the conserved structure of protein motifs, PPI network, cis-regulatory units, and gene structure analysis demonstrated several genetic determinants reflecting the wide range of functional diversity of CtHCT-encoding genes. The observed expression analysis of <em>CtHCT</em> genes in different flowering stages under normal conditions partially highlighted their putative roles in plant growth and development pathways. Moreover, <em>CtHCT</em> genes appeared to be associated with abiotic stress responses as validated by the expression profiling in various flowering phases under light irradiation and MeJA treatment. Altogether, these findings provide new insights into identifying crucial molecular targets associated with plant growth and development and present practical information for understanding abiotic stress-responsive mechanisms in plants.</p> ER -