TY - JOUR AU - ZHOU, Yong AU - HU, Lifang AU - GE, Lingli AU - HE, Peng AU - YANG, Yingui AU - LIU, Shiqiang PY - 2018/12/21 Y2 - 2024/03/29 TI - Isolation and Functional Characterization of an AGAMOUS-LIKE 18 (AGL18) MADS-box Gene from Cucumber (Cucumis sativus L.) JF - Notulae Botanicae Horti Agrobotanici Cluj-Napoca JA - Not Bot Horti Agrobo VL - 47 IS - 2 SE - Research Articles DO - 10.15835/nbha47111345 UR - https://notulaebotanicae.ro/index.php/nbha/article/view/11345 SP - 300-307 AB - <p style="text-align: justify;">MADS-box proteins play vital roles in plant growth and development. However, few studies have addressed the biological functions of MADS-box genes in cucumber. In this study, a MADS-box gene, <em>CsMADS25</em>, was cloned from cucumber (<em>Cucumis sativus</em> L.). The open reading frame (ORF) of <em>CsMADS25 </em>was 810 bp in length and encoded a deduced protein consisting of 269 amino acids with a calculated MW of 30.53 kDa and a theoretical pI of 5.38. Sequence alignment showed that CsMADS25 shared the highest amino acid identity with CmMADS09 from <em>Cucumis melo</em>. Phylogenetic tree analysis indicated that CsMADS25 was clustered with AGL18 proteins with high bootstrap values. qRT-PCR analysis showed that the expression of <em>CsMADS25 </em>was observably regulated by various abiotic stresses and GAtreatments. Overexpression of <em>CsMADS25</em> resulted in dwarf and small-leaf phenotypes in transgenic <em>Arabidopsis</em> plants, and the leaf index value (leaf length/width ratio) of transgenic plants was dramatically increased compared with that of wild-type (WT) plants. These findings suggest that <em>CsMADS25 </em>might play important roles in various developmental processes and in response to abiotic stress of cucumber.</p> ER -