TY - JOUR AU - FAYED, Sayed A. PY - 2015/12/10 Y2 - 2024/03/29 TI - Chemical Composition, Antioxidant, Anticancer Properties and Toxicity Evaluation of Leaf Essential Oil of Cupressus sempervirens JF - Notulae Botanicae Horti Agrobotanici Cluj-Napoca JA - Not Bot Horti Agrobo VL - 43 IS - 2 SE - Research Articles DO - 10.15835/nbha43210070 UR - https://notulaebotanicae.ro/index.php/nbha/article/view/10070 SP - 320-326 AB - <p>The essential oil isolated by hydro-distillation from <em>Cupressus&nbsp; sempervirens</em> (Cypress) leaves was analysed by GC-MS and tested for antioxidant and <em>in vitro</em> as well as <em>in vivo</em> anticancer activities. In addition, the toxicity effect of the essential oil was studied using normal Swiss mice. Eighteen components of Cypress essential oil were identified and the main essential oil components were α-pinene (29.21%), δ3-carene(18.92%), α-cedrol (12.25%), α-terpinolene (7.66%) and limonene (5.50%). <em>Cupressus sempervirens</em> essential oil was able to reduce the stable, purple-colored radical DPPH into yellow-colored DPPH reaching 50% of reduction with IC<sub>50</sub> value = 290.09 μg mL<sup>-1</sup>. The <em>in vitro</em> anticancer activity of the essential oil was studied against two human promyelocytic leukemia cell lines (HL-60 and NB4) and experimental animals model cancer cell line (EACC). Cypress essential oil exerted the highest cytotoxic activity with a LC<sub>50</sub> of 333.79 μg mL<sup>-1</sup> against NB4 followed by HL-60 and EACC cell lines (LC<sub>50</sub> of 365.41, and 372.43 μg mL<sup>-1</sup>, respectively). Regarding <em>in vivo</em> anticancer study, pre-initiation treatment with the essential oil was more effective than initiation and post-initiation treatments respectively on the tumor (EACC) transplanted female mice (increase lifespan (%), decrease total EACC number and increase dead cells). In toxicity study, serum urea, transaminases and lactate dehydrogenase were increased. The results obtained from this study showed that the Cypress essential oil possesses antioxidant and anticancer properties, taking into consideration its mild toxicity.</p> ER -