@article{CHENG_TIAN_ZOU_WU_KUČA_BORA_2020, title={Molecular responses of arbuscular mycorrhizal fungi in tolerating root rot of trifoliate orange}, volume={48}, url={https://notulaebotanicae.ro/index.php/nbha/article/view/11916}, DOI={10.15835/nbha48211916}, abstractNote={<p style="text-align: justify;">Arbuscular mycorrhizal fungi (AMF) enhance plant disease resistance, while the underlying mechanisms in the molecular levels are not yet known. In this study, five-leaf-old trifoliate orange seedlings were inoculated with <em>Funneliformis mosseae</em> for 14 weeks and subsequently were infected by a citrus root rot pathogen <em>Phytophthora parasitica </em>by 7 days. The transcriptome results by Illumina HiSeq 4000 revealed that the percentage of Q30 bases reached 92.99% or above, and 29696 unigenes were annotated in a total of 63531 unigenes. 654 and 103 differentially expressed genes (DEGs) were respectively annotated in AMF-inoculated versus non-AMF-inoculated plants under non-infection and infection with <em>P</em>.<em> parasitica</em>, respectively, whilst these DEGs were related to defense mechanisms, signal transduction mechanisms and secondary metabolites biosynthesis. Forty-two genes were functionally annotated as the putative 'defense mechanism', whilst AMF inoculation induced 1 gene down-regulated and 3 genes up-regulated under <em>P</em>.<em> parasitica</em> infection. AMF inoculation stimulated more genes linked to signal transduction mechanism down-regulated than non-AMF plants. Eight genes were involved in secondary metabolites biosynthesis in AMF versus non-AMF seedlings under <em>P</em>.<em> parasitica</em>-infection conditions. Such transcriptome database provided total information in the molecular levels regarding mycorrhizal roles in tolerating <em>Phytophthora parasitica</em> infection.</p>}, number={2}, journal={Notulae Botanicae Horti Agrobotanici Cluj-Napoca}, author={CHENG, Shen and TIAN, Li and ZOU, Ying-Ning and WU, Qiang-Sheng and KUČA, Kamil and BORA, Popy}, year={2020}, month={Jun.}, pages={558–571} }