Effectiveness of a Peptide-rich Fraction from <i>Xenorhabdus budapestensis</i> Culture against Fire Blight Disease on Apple Blossoms

  • Dávid VOZIK University of Pannonia, Research Institute on Bioengineering, Membrane Technology and Energetics, Egyetem u. 10, H-8200, Veszprém
  • Katalin BÉLAFI-BAKÓ University of Pannonia, Research Institute on Bioengineering, Membrane Technology and Energetics, Egyetem u. 10, H-8200, Veszprém
  • Mária HEVESI Corvinus University of Budapest, Department of Pomology, Ménesi út 29-43, H-1118, Budapest
  • Erzsébet BÖSZÖRMÉNYI Semmelweis University, Department of Epidemiology, Vas u. 17, H-1088, Budapest
  • András FODOR University of Wisconsin-Madison, Department of Bacteriology, 1550 Linden Drive, Madison, Room 4526, WI 53507

Abstract

Erwinia amylovora is one of the most frequently occurred plant pathogenic bacterium. It causes necrosis and blight symptoms on host plants
and it lead to considerable yield losses throughout the world on apple trees. There is no effective chemical treatment is currently available against fire blight. The purpose of the present study was to search a new, alternative control method. The evaluation of the plant protection potential of an enriched fraction of Xenorhabdus budapestensis cell-free conditioned media was investigated. Purified samples were tested in vitro and in planta
against the phytopathogenic bacterium. A reproducible method for isolation of a peptide-rich fraction from Xenorhabdus cell-free conditioned
media was established. The process resulted in 400 mg of dry sample prepared from three litres of Xenorhabdus cell culture. Significant correlation was found between the concentration of the purified preparation and the induced inactivation zones against Erwinia amylovora Ea1 in agar
diffusion test method. The minimum inhibitory concentration and minimum bactericidal concentration of the purified fraction against Ea1
strain were 8 ;g/mL and 16 ;g/mL, respectively. In planta experiments were tested on an apple cultivar (‘Watson Jonathan’) susceptible to fire
blight. The effective range of concentration was 62-200 μg/mL, while treatment with 300 μg/mL and larger amounts caused necrotic symptoms
on the petals of flowers. Current study pointed to the effectiveness of the compounds produced by X. budapestensis against fire blight. The
development of a commercially applicable formulation of these compounds would allow growers to effectively control fire blight in apple and pear
orchards.

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Author Biographies

Katalin BÉLAFI-BAKÓ, University of Pannonia, Research Institute on Bioengineering, Membrane Technology and Energetics, Egyetem u. 10, H-8200, Veszprém

Head of department

Research Institute on Bioengineering, Membrane Technology and Energetics

Mária HEVESI, Corvinus University of Budapest, Department of Pomology, Ménesi út 29-43, H-1118, Budapest

Senior research fellow, retired, CSc

Department of Pomology

Erzsébet BÖSZÖRMÉNYI, Semmelweis University, Department of Epidemiology, Vas u. 17, H-1088, Budapest

Associate professor

Department of Epidemiology

András FODOR, University of Wisconsin-Madison, Department of Bacteriology, 1550 Linden Drive, Madison, Room 4526, WI 53507

Research professor

Published
2015-12-07
How to Cite
VOZIK, D., BÉLAFI-BAKÓ, K., HEVESI, M., BÖSZÖRMÉNYI, E., & FODOR, A. (2015). Effectiveness of a Peptide-rich Fraction from <i>Xenorhabdus budapestensis</i&gt; Culture against Fire Blight Disease on Apple Blossoms. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 43(2), 547-553. https://doi.org/10.15835/nbha4329997
Section
Research Articles