Determination of the <i>In vitro</i> Effect of <i>Trichoderma harzianum</i> on Phytopathogenic Strains of <i>Fusarium oxysporum</i>

  • Hacer Handan ALTINOK University of Erciyes, Faculty of Agriculture, Department of Plant Protection, 38039 Melikgazi, Kayser
  • Oktay ERDOGAN University of Nevsehir Hacı Bektas Veli, Faculty of Engineering and Architecture, Department of Biosystem Engineering, 50300 Nevsehir

Abstract

Fusarium oxysporum is a well-known soil-borne fungi and it is difficult to control their pathogenic strains by conventional strategies. The cultures of two strains of Trichoderma harzianum (T16 and T23) were examined in laboratory conditions and with pot experiments for the control of pathogenic strains of Fusarium oxysporum f. sp. melongenae (Fomg), Fusarium oxysporum f. sp. lycopersici (Fol), Fusarium oxysporum f. sp. niveum (Fon) and F. oxysporum f. sp. melonis (Fom). The T16 and T23 strains showed significant inhibition of mycelial growth in the pathogenic strains of F. oxysporum and the maximum inhibition were recorded when the T. harzianum strain T16 was used (72.69%). Both T. harzianum strains produced volatile and non-volatile metabolites that inhibited growth of F. oxysporum strains on PDA medium. In vitro colonization study demonstrated the root-colonizing ability of these antagonists. The interaction between T. harzianum isolates (T16 and T23) and pathogenic F. oxysporum hyphae showed no overgrowth, hyphal coiling, cell wall degradation or any hyphal penetration around any of the tested F. oxysporum hyphae. Pre-treatment of soil with T16 significantly reduced the severity of Fusarium wilt disease. The disease severity in control plants reached to 90-95% whereas those of the T16-Fomg and T16-Fol treated seedlings of eggplants were 37.74% and 47.12%, respectively, on the 21st day. In this study, while both T. harzianum isolates had a considerable antagonistic effect on the tested pathogens, T16 was found to be more successful than T23. The strong repressive effect of T. harzianum (T16) towards pathogenic Fusarium oxsporum can be applied in biological control of these pathogens.

Metrics

Metrics Loading ...
Published
2015-12-10
How to Cite
ALTINOK, H. H., & ERDOGAN, O. (2015). Determination of the <i>In vitro</i> Effect of <i>Trichoderma harzianum</i> on Phytopathogenic Strains of <i>Fusarium oxysporum</i&gt;. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 43(2), 494-500. https://doi.org/10.15835/nbha4329788
Section
Research Articles