Using DNA Barcoding to Identify the Genus <i>Lolium</i>

  • Shenmao WU Shanghai University, School of Life Sciences, No 333, Nanchen road, Shanghai
  • Liping YIN Shanghai Entry-Exit Inspection and Quarantine Bureau, Technical Center for Animal, Plant and Food Inspection and Quarantine, No 1208, Minsheng road, Shanghai
  • Zhirui DENG Shanghai University, School of Life Sciences, No 333, Nanchen road, Shanghai
  • Qin CHEN Shanghai University, School of Life Sciences, No 333, Nanchen road, Shanghai
  • Yining FU Shanghai Entry-Exit Inspection and Quarantine Bureau, Technical Center for Animal, Plant and Food Inspection and Quarantine, No 1208, Minsheng road, Shanghai
  • Huajie XUE Shanghai Entry-Exit Inspection and Quarantine Bureau, Technical Center for Animal, Plant and Food Inspection and Quarantine, No 1208, Minsheng road, Shanghai

Abstract

Seeds of the genus Lolium are difficult to identify based on morphology for morphological likeness and some physical deformation such as friction and flattening during storage and transport. DNA barcoding, a newly-established method, has been used to discriminate a variety of agricultural crops with its own advantages. In present study, DNA barcodes for the genus Lolium were investigated for the first time. DNA sequences of psbA-trnH, rbcL, atpF-atpH, and the ITS2 region were evaluated for their ability to differentiate Lolium from the related genus Festuca. As confirmed by inter-intraspecific divergence and Kimura 2 parameter analysis, the greatest divergence existed in ITS2, followed by psbA-trnH. On the contrary, rbcL and atpF-atpH possessed poor genetic variation of 0-0.0115, and was relatively difficult in discrimination of genus Lolium. For ITS2 sequence, no inter-intraspecific distance overlaps were observed and each species has a distinct barcoding gap. ITS2 could effectively discriminate all species based on a neighbor-joining tree. Thus, the ITS2 region is a candidate for DNA barcoding of Lolium.

Metrics

Metrics Loading ...
Published
2015-12-10
How to Cite
WU, S., YIN, L., DENG, Z., CHEN, Q., FU, Y., & XUE, H. (2015). Using DNA Barcoding to Identify the Genus <i>Lolium</i&gt;. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 43(2), 536-541. https://doi.org/10.15835/nbha4329747
Section
Research Articles