Development of a SCAR Marker for Rapid Identification of New Kentucky Bluegrass Breeding Lines

  • Xiaojun YUAN School of Life Science, Shanghai University, Shanghai 200444; Shanghai Key Laboratory of Bio-Energy Crops, Shanghai University
  • Yali HE School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240
  • Junjie HUANG School of Life Science, Shanghai University, Shanghai 200444; Shanghai Key Laboratory of Bio-Energy Crops, Shanghai University
  • Wen HU School of Life Science, Shanghai University, Shanghai 200444; Shanghai Key Laboratory of Bio-Energy Crops, Shanghai University
  • Huanhuan ZHOU School of Life Science, Shanghai University, Shanghai 200444; Shanghai Key Laboratory of Bio-Energy Crops, Shanghai University
  • Qiongyu GAO School of Life Science, Shanghai University, Shanghai 200444; Shanghai Key Laboratory of Bio-Energy Crops, Shanghai University
  • Shumin ZHOU School of Life Science, Shanghai University, Shanghai 200444; Shanghai Key Laboratory of Bio-Energy Crops, Shanghai University

Abstract

As a commonly used turfgrass, Kentucky bluegrass (Poa pratensis L.) (KBG) has many commercially available cultivars for production. After several years of screening, two new lines were obtained (‘KBG03’ and ‘KBG04’), which have high tolerance to summer. The study showed that the two lines revealed similar morphological characteristics, with light green leaf color, narrow leaf blade, high plant height and light 1,000-grain weight. A total of 400 random amplified polymorphic DNA (RAPD) primers and 256 sequence-related amplified polymorphism (SRAP) primer combinations were screened among the two lines and other 4 imported commercial cultivars. The percentages of polymorphic sites were 65.5% (RAPD) and 22.6% (SRAP) respectively. By cluster analysis of RAPD and SRAP data, the dendrogram at a similarity of 0.29 gave two main clusters, of which one group had 4 commercial cultivars, and the other had the two new breeding lines. Furthermore, one specific band of ‘KBG04’ was successfully converted into a dominant sequence characterized amplified region marker (SCAR196). Then the SCAR marker was verified by 39 KBG DNA samples, including imported varieties, domestic varieties and self-breeding lines of our laboratory, and it exhibited high consistency with the original RAPD polymorphic amplification. The results showed that the SCAR marker can be used to distinguish the new line ‘KBG04’ from numerous KBG germplasms, which would be useful for cultivar identification and property rights protection in the future.

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Published
2015-06-16
How to Cite
YUAN, X., HE, Y., HUANG, J., HU, W., ZHOU, H., GAO, Q., & ZHOU, S. (2015). Development of a SCAR Marker for Rapid Identification of New Kentucky Bluegrass Breeding Lines. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 43(1), 79-85. https://doi.org/10.15835/nbha4319664
Section
Research Articles