Genetic Relationships Among Representatives of Dasypyrum, Secale and Triticum Species Revealed with RAPD and ISSR Markers

Authors

  • Agnieszka GRADZIELEWSKA University of Life Sciences in Lublin, Institute of Plant Genetics, Breeding and Biotechnology, Lublin (PL)
  • Mirosław TYRKA Rzeszów University of Technology, Department of Biochemistry and Biotechnology, Rzeszów (PL)
  • Justyna LESNIOWSKA-NOWAK University of Life Sciences in Lublin, Institute of Plant Genetics, Breeding and Biotechnology, Lublin (PL)
  • Justyna NAZARUK University of Life Sciences in Lublin, Institute of Plant Genetics, Breeding and Biotechnology, Lublin (PL)

DOI:

https://doi.org/10.15835/nbha4229662

Abstract

In this study the genetic similarity among Dasypyrum, Secale andTriticum species with RAPDs and ISSRs was analyzed. To show a level of similarity between the species, 12 populations of Dasypyrum (11 D. villosum and 1 D. breviaristatum), together with 12 accessions belonging to 3 Secale species and 12 accessions from 4 Triticum species were used. Genetic distances (GD) and bootstrap values were calculated and PCA analysis was conducted to present the relationships among the species. To estimate the genetic structure among and inside genera, as well as population differentiation, gene diversity (He), total genetic variation (Ht) and Wright’s fixation index (Fst) were computed. The highest values were found in Triticum, within which Ht was equal to 0.332±0.023 and Fst was 0.42. It confirmed that the material studied was highly differentiated. Both systems found Dasypyrum more related with Triticum, as compared to Secale. With RAPDs, genetic distance (GD) between Triticum and Dasypyrum was 0.435, respectively 0.460 for Secale vs Dasypyrum. In the case of ISSRs these values were 0.374 and 0.407, respectively. Despite the fact that the difference between the two GD indices was insignificant, one should not exclude the possibility of successful hybridization of Dasypyrum and Secale, especially when using bridge species.

Author Biographies

Agnieszka GRADZIELEWSKA, University of Life Sciences in Lublin, Institute of Plant Genetics, Breeding and Biotechnology, Lublin

Institute of Plant Genetics, Breeding and Biotechnology

Mirosław TYRKA, Rzeszów University of Technology, Department of Biochemistry and Biotechnology, Rzeszów

Department of Biochemistry and Biotechnology

Justyna LESNIOWSKA-NOWAK, University of Life Sciences in Lublin, Institute of Plant Genetics, Breeding and Biotechnology, Lublin

Institute of Plant Genetics, Breeding and Biotechnology

Justyna NAZARUK, University of Life Sciences in Lublin, Institute of Plant Genetics, Breeding and Biotechnology, Lublin

Institute of Plant Genetics, Breeding and Biotechnology

Downloads

Published

2014-12-02

How to Cite

GRADZIELEWSKA, A., TYRKA, M., LESNIOWSKA-NOWAK, J., & NAZARUK, J. (2014). Genetic Relationships Among Representatives of Dasypyrum, Secale and Triticum Species Revealed with RAPD and ISSR Markers. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 42(2), 420–430. https://doi.org/10.15835/nbha4229662

Issue

Section

Research Articles
CITATION
DOI: 10.15835/nbha4229662

Most read articles by the same author(s)