Genetic Relationships Among Representatives of Dasypyrum, Secale and Triticum Species Revealed with RAPD and ISSR Markers

  • Agnieszka GRADZIELEWSKA University of Life Sciences in Lublin, Institute of Plant Genetics, Breeding and Biotechnology, Lublin
  • Mirosław TYRKA Rzeszów University of Technology, Department of Biochemistry and Biotechnology, Rzeszów
  • Justyna LESNIOWSKA-NOWAK University of Life Sciences in Lublin, Institute of Plant Genetics, Breeding and Biotechnology, Lublin
  • Justyna NAZARUK University of Life Sciences in Lublin, Institute of Plant Genetics, Breeding and Biotechnology, Lublin

Abstract

In this study the genetic similarity among Dasypyrum, Secale andTriticum species with RAPDs and ISSRs was analyzed. To show a level of similarity between the species, 12 populations of Dasypyrum (11 D. villosum and 1 D. breviaristatum), together with 12 accessions belonging to 3 Secale species and 12 accessions from 4 Triticum species were used. Genetic distances (GD) and bootstrap values were calculated and PCA analysis was conducted to present the relationships among the species. To estimate the genetic structure among and inside genera, as well as population differentiation, gene diversity (He), total genetic variation (Ht) and Wright’s fixation index (Fst) were computed. The highest values were found in Triticum, within which Ht was equal to 0.332±0.023 and Fst was 0.42. It confirmed that the material studied was highly differentiated. Both systems found Dasypyrum more related with Triticum, as compared to Secale. With RAPDs, genetic distance (GD) between Triticum and Dasypyrum was 0.435, respectively 0.460 for Secale vs Dasypyrum. In the case of ISSRs these values were 0.374 and 0.407, respectively. Despite the fact that the difference between the two GD indices was insignificant, one should not exclude the possibility of successful hybridization of Dasypyrum and Secale, especially when using bridge species.

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Author Biographies

Agnieszka GRADZIELEWSKA, University of Life Sciences in Lublin, Institute of Plant Genetics, Breeding and Biotechnology, Lublin
Institute of Plant Genetics, Breeding and Biotechnology
Mirosław TYRKA, Rzeszów University of Technology, Department of Biochemistry and Biotechnology, Rzeszów
Department of Biochemistry and Biotechnology
Justyna LESNIOWSKA-NOWAK, University of Life Sciences in Lublin, Institute of Plant Genetics, Breeding and Biotechnology, Lublin
Institute of Plant Genetics, Breeding and Biotechnology
Justyna NAZARUK, University of Life Sciences in Lublin, Institute of Plant Genetics, Breeding and Biotechnology, Lublin
Institute of Plant Genetics, Breeding and Biotechnology
Published
2014-12-02
How to Cite
GRADZIELEWSKA, A., TYRKA, M., LESNIOWSKA-NOWAK, J., & NAZARUK, J. (2014). Genetic Relationships Among Representatives of Dasypyrum, Secale and Triticum Species Revealed with RAPD and ISSR Markers. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 42(2), 420-430. https://doi.org/10.15835/nbha4229662
Section
Research Articles