Genetic Similarity Assessment among Selected Naked Oat Cultivars and Breeding Lines Using ISSR Markers

  • Edyta PACZOS-GRZEDA University of Life Sciences in Lublin, Institute of Plant Genetics, Breeding and Biotechnology, Akademicka Str 15, 20-950 Lublin
  • Piotr Tomasz BEDNAREK Plant Breeding and Acclimatization Institute – NRI, Radzikow, 05-870 Blonie
  • Aneta KOROLUK University of Life Sciences in Lublin, Institute of Plant Genetics, Breeding and Biotechnology, Akademicka Str 15, 20-950 Lublin
  • Zygmunt NITA Strzelce Plant Breeding Company, Glowna Str 20, 99-307 Strzelce
  • Zofia BANASZAK DANKO Plant Breeding Company, Choryn 27, 64-000 Koscian
  • Andrzej BICHONSKI Malopolska Plant Breeding Company, Polanowice, 32-090 Slomniki
  • Marek CHMIEL Malopolska Plant Breeding Company, Polanowice, 32-090 Slomniki
  • Agnieszka GRADZIELEWSKA University of Life Sciences in Lublin, Institute of Plant Genetics, Breeding and Biotechnology, Akademicka Str 15, 20-950 Lublin
  • Katarzyna NOWACZYK DANKO Plant Breeding Company, Choryn 27, 64-000 Koscian
  • Aleksandra SZOLKOWSKA DANKO Plant Breeding Company, Choryn 27, 64-000 Koscian
  • Krystyna WERWINSKA Strzelce Plant Breeding Company, Glowna Str 20, 99-307 Strzelce
  • Patrycja WIECZOREK Malopolska Plant Breeding Company, Polanowice, 32-090 Slomniki

Abstract

Naked oat refers to a variety of Avena sativa with lemma and palea separating from the grains. Its spikelets are multiflorous and morphologically different from the husked oat. Problems with preharvest sprouting, threshability, rancidity, a wide range of kernel sizes, as well as its relatively low tolerance to limited soil water content, are its main drawbacks. Nevertheless it could be an alternative to a conventional oat. Unfortunately, its genetic variation is still poorly recognized.  In the given study a set of 26 naked oat cultivars and lines were analyzed with 25 inter-simple sequence repeats (ISSR) primers that amplified as many as 429 DNA fragments among which 204 were polymorphic. The average number of markers amplified per primer pair and polymorphism information content (PIC) value equaled to eight and 0.23, respectively. Forty four unique PCR products were identified for different genotypes. While Unweighted Pair-Group Method with Arithmetic Mean failed to distinguish the materials into main clusters it demonstrated that cultivars ‘Akt’, ‘Polar’, ‘Cacko’, ‘Siwek’, ‘Nagus’ and most of the DC lines were within a single group. Moreover, the cultivars that were closely related based on their breeding pedigree (related to ‘Akt’) were close to each other. Principal Coordinate Analysis explained 54.1% of variance and was in good agreement with the UPGMA. ISSR markers could be used for the evaluation of genetic similarity of cultivars and lines as well as the differentiation of individual genotypes. This study demonstrated that the available A. sativa naked type genetic pool is relatively wide and have the potential for further breeding progress.

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Published
2014-06-03
How to Cite
PACZOS-GRZEDA, E., BEDNAREK, P. T., KOROLUK, A., NITA, Z., BANASZAK, Z., BICHONSKI, A., CHMIEL, M., GRADZIELEWSKA, A., NOWACZYK, K., SZOLKOWSKA, A., WERWINSKA, K., & WIECZOREK, P. (2014). Genetic Similarity Assessment among Selected Naked Oat Cultivars and Breeding Lines Using ISSR Markers. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 42(1), 66-72. https://doi.org/10.15835/nbha4219470
Section
Research Articles