Promoter Analysis and Transcriptional Profiling of Ginkgo biloba 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (GbHMGR) gene in Abiotic Stress Responses

  • Yongling LIAO College of Forestry, Nanjing Forest University, Nanjing, 210037
  • Feng XU College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025
  • Xiaohua HUANG Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang Normal University, Huanggang, 438000
  • Weiwei ZHANG College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025
  • Hua CHENG Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang Normal University, Huanggang, 438000
  • Linling LI Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang Normal University, Huanggang, 438000
  • Shuiyuan CHENG School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023
  • Yongbao SHEN College of Forestry, Nanjing Forest University, Nanjing, 210037

Abstract

The terpene trilactones (TTLs) are believed to be important for the pharmacological properties of Ginkgo biloba leaves extract. 3-Hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) is a critical enzyme involved in the biosynthetic pathway of TTLs. In this study, an 1.2-kb fragment of 5’ flanking region of the HMGR gene (GbHMGR), was isolated from G. biloba by genome walking. Extensive sequence analysis revealed the presence of evolutionarily conserved and over-represented putative cis-acting elements in light-regulated transcription,  hormone signaling (gibberellic acid, jasmonate and salicylic acid), elicitor and stress responses (cold/dehydration responses), and plant defense signaling (W-box/WRKY) that are common to the promoter region of GbHMGR. EMSA analysis suggested possible functionality of W-box in GbHMGR promoter region. The behavior of gene transcripts in ginkgo callus upon light, low temperature, MeJA and SA treatments further verified the regulatory function of GbHMGR promoter. A significant positive relationship between gene expression level and total TTL contents suggested that GbHMGR might be one of key genes involved in TTL biosynthesis in G. biloba.

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Published
2015-05-16
How to Cite
LIAO, Y., XU, F., HUANG, X., ZHANG, W., CHENG, H., LI, L., CHENG, S., & SHEN, Y. (2015). Promoter Analysis and Transcriptional Profiling of Ginkgo biloba 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (GbHMGR) gene in Abiotic Stress Responses. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 43(1), 25-34. https://doi.org/10.15835/nbha4319416
Section
Research Articles

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