Production of Flavonoids and Terpene Lactones from Optimized <i>Ginkgo biloba</i> Tissue Culture
AbstractThis study investigated the effects of various culture conditions on the growth and the production of flavonoids and terpene lactones in the callus of Ginkgo biloba. Callus induced from embryos displayed distinct morphological and physiological responses. MS medium with different plant growth regulators showed a significant effect on the quality and growth of callus. The optimal medium for inducing embryo-derived callus was MS with 2.0 mg/L naphthalene acetic acid (NAA) and 2.0 mg/L 6-benzylaminopurine (6-BA), and the culture medium MS+NAA (2.0 mg/L)+6-BA (1.0 mg/L) was better for the subculture of callus than other culture media tested in this study. In addition, both plant growth regulators and subculturing cycle strongly influenced the production of flavonoids and terpene lactones in the callus. The best subculturing cycle and the optimum culture medium for production of flavonoids and terpene lactones was 30 d and MS+NAA (2.0 mg/L)+6-BA (1.0 mg/L), respectively. These findings provided an important technical support for obtaining the callus cell line from G. biloba embryo that is the richest in flavonoids and terpene lactones.
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