Development of SCAR Marker Related to Summer Stress Tolerance in Tall Fescue (<i>Festuca arundinacea</i>)</span>

  • Xiaojun YUAN School of Life Science, Shanghai University, Shanghai 200444; Shanghai Key Laboratory of Bio-Energy Crops, Shanghai University, China 2School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240
  • Zhenjing BAO School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240
  • Yali HE School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240
  • Qun CHEN School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240
  • Gang WANG School of Life Science, Shanghai University, Shanghai 200444; Shanghai Key Laboratory of Bio-Energy Crops, Shanghai University

Abstract

Summer stress tolerance (SST) is one of the most important breeding objectives in tall fescue (Festuca arundinacea), an important perennial cool-season grass. However, breeding for better SST is generally complicated by the many environmental factors involved during the growing season. Utilizing the bulked segregant analysis (BSA), we were able to identify one marker related to SST from 100 inter-simple sequence repeat (ISSR) markers and 800 random amplified polymorphic DNA (RAPD) markers, and successfully developed a dominant sequence characterized amplified region (SCAR) marker T_SC856 from the UBC856 sequence. Furthermore, the SCAR marker was tested in different clones of new populations, which were identified under complex summer stress (high temperature and humidity, Pythium blight, and brown patch), and it exhibited relatively high consistency (77%) with the phenotype. We believe that with more markers obtained in the future, better efficiency is likely to be achieved in breeding for improved SST in tall fescue and possibly other species as well. Further studies that analyze the factors relating to the SCAR marker are needed.

Metrics

Metrics Loading ...
Published
2014-06-03
How to Cite
YUAN, X., BAO, Z., HE, Y., CHEN, Q., & WANG, G. (2014). Development of SCAR Marker Related to Summer Stress Tolerance in Tall Fescue (<i>Festuca arundinacea</i>)</span&gt;. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 42(1), 81-87. https://doi.org/10.15835/nbha4219381
Section
Research Articles

Most read articles by the same author(s)