Molecular Characterization and Analysis of the Greek Citrus Germplasm

  • Constantinos TRIPOLITSIOTIS Agricultural University of Athens, Department of Crop Science, Pomology Laboratory, Iera Odos 75, 118 55 Athens
  • Nikolaos NIKOLOUDAKIS Hellenic Ministry of Rural Development and Food, Vegetative Propagation Material Control Station
  • Athanasios LINOS Agricultural University of Athens, Department of Crop Science, Pomology Laboratory, Iera Odos 75, 118 55 Athens
  • Marianna HAGIDIMITRIOU Agricultural University of Athens-Greece

Abstract

Molecular markers were used to evaluate genetic similarity among 36 citrus accessionsc (C. sinensis, C. reticulata, C. limon and cultivars) of the Greek national clonal germplasm repository located in Poros. In total, nine RAPD and five ISSR primers produced 262 loci, of which 236 were polymorphic. Both techniques were proven to be equally analytical with an average discrimination power above 0.9. The RAPD and ISSR markers were highly correlated and clustering among them had high correspondence. Citrus accessions formed separate clusters according to their species, even though sweet orange and mandarin cultivars revealed high affinity, while lemons were more divergent. C. sinensis genotypes had the highest intragenetic affinity suggesting that cultivated oranges have a narrow genetic base. On the contrary, the C. reticulata group was the most variable. In general, Greek varieties exhibited a distinctive genetic background that could be discriminated from foreign established cultivars using the mentioned molecular techniques. This information could facilitate their use in future Citrus spp. breeding programs.

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Author Biography

Marianna HAGIDIMITRIOU, Agricultural University of Athens-Greece
Pomology laborarory Assistant Professor
Published
2013-12-06
How to Cite
TRIPOLITSIOTIS, C., NIKOLOUDAKIS, N., LINOS, A., & HAGIDIMITRIOU, M. (2013). Molecular Characterization and Analysis of the Greek Citrus Germplasm. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41(2), 463-471. https://doi.org/10.15835/nbha4129302
Section
Research Articles

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