Elimination of <i>Grapevine fleck virus</i> by <i>in vitro</i> Chemotherapy

  • Ionela Catalina GUȚĂ National Research and Development Institute for Biotechnology in Horticulture, 37 Calea Bucuresti, Ștefanesti-Argeș
  • Elena-Cocuța BUCIUMEANU National Research and Development Institute for Biotechnology in Horticulture, 37 Calea Bucuresti, Stefanesti-Arges
  • Emilia VIȘOIU National Research and Development Institute for Biotechnology in Horticulture, 37 Calea Bucuresti, Stefanesti-Arges

Abstract

Grapevine fleck virus produces a ubiquitous disease, latent in European grapevine varieties (Vitis vinifera L.) and in most American rootstocks, being found in all viticultural countries, in simple or complex infections with other more dangerous viruses. Different techniques for sanitation showed controversial results regarding fleck elimination in grapevine. In vitro culture of ‘Tamâioasa româneasca’ 3-2-2 with fleck virus and ‘Burgund’ 63 Mn with double infection (Grapevine fleck virus and Grapevine virus A), naturally infected have been submitted to chemotherapy using a combination of ribavirin and oseltamivir in three concentration variants (V1-40 mg/L ribavirin + 40 mg/L oseltamivir; V2-20 mg/L ribavirin + 40 mg/L oseltamivir; V3-20 mg/L ribavirin + 80 mg/L oseltamivir) and three consecutive subcultures. The plants regenerated after each subculture were evaluated by ELISA from the viewpoint of virus elimination and the RT-PCR was used for confirmation of the diagnostic. Due the phytotoxic effect of
viricides, the multiplication rate decreased on experimental variants in the next subculture comparatively to the control, but no mortality of explants has been registered. Grapevine fleck virus has been 100% eliminated both from simple and mixed infections on all variants by in vitro chemotherapy, under the simultaneous action of two viricides. Unsatisfactory results have been achieved with Grapevine virus A elimination.

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Author Biographies

Ionela Catalina GUȚĂ, National Research and Development Institute for Biotechnology in Horticulture, 37 Calea Bucuresti, Ștefanesti-Argeș
Research department-Virology
Elena-Cocuța BUCIUMEANU, National Research and Development Institute for Biotechnology in Horticulture, 37 Calea Bucuresti, Stefanesti-Arges
Research department-Virology
Emilia VIȘOIU, National Research and Development Institute for Biotechnology in Horticulture, 37 Calea Bucuresti, Stefanesti-Arges
Research department- Plant biotechnology
Published
2014-06-03
How to Cite
GUȚĂ, I. C., BUCIUMEANU, E.-C., & VIȘOIU, E. (2014). Elimination of <i>Grapevine fleck virus</i> by <i>in vitro</i&gt; Chemotherapy. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 42(1), 115-118. https://doi.org/10.15835/nbha4219227
Section
Research Articles