The Phylogenetic Analysis and Identification of Sorghum halepense and Related Species Based on the Chi-B Partial Sequence

  • Chen QIN School of Life Science, Shanghai University, Shanghai, 200444
  • Wang WEI School of Life Science, Shanghai University, Shanghai, 200444
  • Radu E. SESTRAS University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, 3-5 Manastur St., 400372
Keywords: S. halepense, phylogenetic analysis, Chi-B

Abstract

Chi-B partial sequences of Sorghum halepense and its relatives were amplified with primer D16/D18 derived from Chi-B of the Sorghum genus. The results showed that the length varied from 567 bp to 607 bp, while phylogenetic relationship analysis by "DNASTAR" software showed that all materials were separated into three sections. The first section included S. halepense, S. almum and S. silk with a similarity of 96.9~100%, the S. bicolor, S. propinquum and S. arumdinaceum species were in sections of two with 93.0~95.3% similarity, the genetic distance between S. nitidum and the others was longer than in the case of the others, with a similarity of 69.0~71.0%. With accession-special primer D51/D55, S. halepense and S. almum could be identified by PCR.

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How to Cite
QIN, C., WEI, W., & SESTRAS, R. E. (1). The Phylogenetic Analysis and Identification of Sorghum halepense and Related Species Based on the Chi-B Partial Sequence. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 36(1), 55-58. https://doi.org/10.15835/nbha36192
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Research Articles

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