RAPD Marker Conversion into a SCAR Marker for Rapid Identification of Johnsongrass [Sorghum halepense (L.) Pers.]

  • Wenju ZHANG
  • Shasha WEI
  • Liping YIN
  • Zhirui DENG
  • Jianping YI
  • Renqi WU
  • Qin CHEN

Abstract

Johnsongrass [Sorghum halepense (L.) Pers.] is a malignant weed in the world, threatening biodiversity at invaded habitats in more than 50 countries. Because of similarity in morphological characters, S. halepense and its relatives, S. almum, S. nitidum, S. propinquum, S. sudanense, and S. bicolor, etc. was difficult to identify. As a supplementary methodolgy for morphology identification, a molecular detection method was established. Sequence Characterized Amplified Regions (SCAR) marker is a recent established, reliabile, and stable molecular marker based on RAPD maker, an effective way for germplasm identification. In this study, one specific band of S.halepense was screened by 163 pairs of RAPD primers. According to the sequences of the band, a pair of special SCAR primers SH1/SH2 was designed and verified by 65 Sorghum DNA samples from all over the world. The results showed SCAR primers SH1/SH2 can be used to distinguish S.halepense and its relatives rapidly with three exceptions of Australia geotypes.

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Author Biographies

Wenju ZHANG
Shasha WEI
Liping YIN
Zhirui DENG
Jianping YI
Renqi WU
Qin CHEN
Published
2013-05-28
How to Cite
ZHANG, W., WEI, S., YIN, L., DENG, Z., YI, J., WU, R., & CHEN, Q. (2013). RAPD Marker Conversion into a SCAR Marker for Rapid Identification of Johnsongrass [Sorghum halepense (L.) Pers.]. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41(1), 306-312. https://doi.org/10.15835/nbha4119001
Section
Research Articles