Physiological and Biochemical Effects of γ-Irradiation on Cowpea Plants (Vigna sinensis) under Salt Stress

  • Hossam S. EL-BELTAGI Department of Biochemistry, Faculty of Agriculture, Cairo University
  • Heba I. MOHAMED Biological and Geological Sciences Department, Faculty of Education, Ain Shams University,
  • Abdel Haleem M.A. MOHAMMED Biological and Geological Sciences Department, Faculty of Education, Ain Shams University,
  • Laila M. ZAKI Biological and Geological Sciences Department, Faculty of Education, Ain Shams University,
  • Asmaa M. MOGAZY Biological and Geological Sciences Department, Faculty of Education, Ain Shams University,

Abstract

Soil salinity is one of the most severe factors limiting growth and physiological response in cowpea plants. In this study, the possible role of γ-irradiation in alleviating soil salinity stress during plant growth was investigated. Increasing salinity in the soil (25, 50, 100 and 200 mM NaCl) decreased plant growth, photosynthetic pigments content, total carbohydrate content and mineral uptake compared to control, while increased total phenol content, proline, total free amino acids and lipid peroxidation. Seed irradiation with gamma rays significantly increased plant growth, photosynthetic pigments, total carbohydrate, total phenol, proline, total free amino acids and the contents of N, P, K +, Ca+2 and Mg+2 compared to non irradiated ones under salinity. On the other hand, irradiation with gamma rays decreased lipid peroxidation, Na+ and Cl- contents which may contribute in part to activate processes involved in the alleviation of the harmful effect of salt at all concentrations used (25, 50 and 100 mM) except at the high concentration (200 mM). Electrophoretic studies of α-esterase, β-esterase, polyphenol oxidase, peroxidase and acid phosphatase isozymes showed wide variations in their intensities among all treatments.

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Author Biographies

Hossam S. EL-BELTAGI, Department of Biochemistry, Faculty of Agriculture, Cairo University
Department of Biochemistry, Faculty of Agriculture, Cairo University
Heba I. MOHAMED, Biological and Geological Sciences Department, Faculty of Education, Ain Shams University,
Biological and Geological Sciences Department, Faculty of Education, Ain Shams University,
Abdel Haleem M.A. MOHAMMED, Biological and Geological Sciences Department, Faculty of Education, Ain Shams University,
Biological and Geological Sciences Department, Faculty of Education, Ain Shams University,
Laila M. ZAKI, Biological and Geological Sciences Department, Faculty of Education, Ain Shams University,
Biological and Geological Sciences Department, Faculty of Education, Ain Shams University,
Asmaa M. MOGAZY, Biological and Geological Sciences Department, Faculty of Education, Ain Shams University,
Biological and Geological Sciences Department, Faculty of Education, Ain Shams University,
Published
2013-05-28
How to Cite
EL-BELTAGI, H. S., MOHAMED, H. I., MOHAMMED, A. H. M., ZAKI, L. M., & MOGAZY, A. M. (2013). Physiological and Biochemical Effects of γ-Irradiation on Cowpea Plants (Vigna sinensis) under Salt Stress. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41(1), 104-114. https://doi.org/10.15835/nbha4118927
Section
Research Articles