Evaluation of Six Methods for Extraction of Total RNA from Loquat

  • Zhang LING College of Horticulture, South China Agricultural University, Guangzhou 510642
  • Zhang ZHIKE College of Horticulture, South China Agricultural University, Guangzhou 510642
  • Lin SHUNQUAN College of Horticulture, South China Agricultural University, Guangzhou 510642
  • Zheng TINGTING College of Horticulture, South China Agricultural University, Guangzhou 510642
  • Yang XIANGHUI College of Horticulture, South China Agricultural University, Guangzhou 510642

Abstract

 

Trizol extraction for polysaccharide-rich plant tissue was unsuitable for isolating total RNA from loquat fruit. CTAB-LiCl extraction was improved with pretreatment using washing buffer with 80% ethanol and 70% acetone. The RNA isolated by this protocol from different loquat fruit tissue at various development periods, was high in purity (A260/A280 ratio ranged from 1.81 to 1.99 and A260/A230 ratio was over 2.0), and high in yield.

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Author Biographies

Zhang LING, College of Horticulture, South China Agricultural University, Guangzhou 510642
Zhang ZHIKE, College of Horticulture, South China Agricultural University, Guangzhou 510642
Lin SHUNQUAN, College of Horticulture, South China Agricultural University, Guangzhou 510642
Zheng TINGTING, College of Horticulture, South China Agricultural University, Guangzhou 510642
Yang XIANGHUI, College of Horticulture, South China Agricultural University, Guangzhou 510642
College of Horticulture, associate professor
Published
2013-05-28
How to Cite
LING, Z., ZHIKE, Z., SHUNQUAN, L., TINGTING, Z., & XIANGHUI, Y. (2013). Evaluation of Six Methods for Extraction of Total RNA from Loquat. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41(1), 313-316. https://doi.org/10.15835/nbha4118233
Section
Research Articles