DNA Fingerprinting and Genotyping of Cotton Varieties Using SSR Markers

  • Mohammad Nazrul ISLAM Sher-e-Bangla Agricultural University, Department of Biotechnology, Dhaka
  • Md. Rezwan MOLLA Agricultural Research Institute, Plant Genetic Resources Centre, Molecular Biology Laboratory, Joydebpur, Gazipur-1701
  • Md. Motiar ROHMAN Bangladesh Agricultural Research Institute, Plant Breeding Division, Molecular Breeding Laboratory, Joydebpur, Gazipur-1701
  • Mirza HASANUZZAMAN Sher-e-Bangla Agricultural University, Department of Agronomy, Faculty of Agriculture, Dhaka-1207
  • Shah Mohammad Naimul ISLAM Bangabandhu Sheikh Mujibur Rahman Agricultural University, Department of Biotechnology, Gazipur
  • Lutfur RAHMAN Bangladesh Agricultural University, Department of Genetics and Plant Breeding, Mymenisingh

Abstract

DNA fingerprinting and genetic diversity analysis helps direct selective breeding and conservation of plant species. Since simple sequence repeats (SSR) markers are co-dominant, they can predict level of genetic diversity and thereby protect plant genetic resources of a region. Keeping the aforesaid rationale in mind, we worked on molecular characterization of eight cotton varieties in Bangladesh using simple sequence repeat (SSR) or microsatellite DNA markers. All the three microsatellite DNA markers were found to be polymorphic, extracting a total of eight alleles with an average of 2.67 alleles per locus in the present study. Allele sizes were as 149-155 bp, 178-198 bp and 140-202 bp for the loci BNL1551, BNL1721 and BNL2960, respectively. Polymorphism Information Content (PIC) values were ranged from 0.469 to 0.531. UPGMA dendrogram separated 8 varieties of cotton into two clusters. One cluster contained six varieties CB-1, CB-2, CB-3, CB-7, CB-9 and CB-10 while other two varieties CB-5 and HC 1 formed another cluster. The findings of this study would provide a useful guide for selecting specific germplasm with distinct genetic background for diversifying cotton breeding program in Bangladesh.

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Author Biography

Mirza HASANUZZAMAN, Sher-e-Bangla Agricultural University, Department of Agronomy, Faculty of Agriculture, Dhaka-1207
Assistant Professor
Published
2012-09-26
How to Cite
ISLAM, M. N., MOLLA, M. R., ROHMAN, M. M., HASANUZZAMAN, M., ISLAM, S. M. N., & RAHMAN, L. (2012). DNA Fingerprinting and Genotyping of Cotton Varieties Using SSR Markers. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 40(2), 261-265. https://doi.org/10.15835/nbha4028166
Section
Research Articles