Quantitative Determination of Arbutin and Hydroquinone in Different Plant Materials by HPLC
AbstractA simple, fast method of high-performance liquid chromatography for the determination and quantification of arbutin andÂ hydroquinone in many different raw materials was developed and validated. The optimum conditions for the separation and detectionÂ of these two constituents were achieved on a LiChro-CARD 125-4 SuperspherÂ®100 RP-18 column with the water-methanol (gradientÂ elution) mobile phase and recorded at 289 nm. The purities of peaks were verified by PDA analysis of impurities. The results of validationÂ have shown that the HPLC method is stable and accurate for the simultaneous determination of arbutin and hydroquinone in extractsÂ from various plants. The developed method gave a good sensitivity (LOD 1Âµg/ml for arbutin and 0.49 Âµg/ml for hydroquinone) withÂ linearity R2Â >0.9993 (for both). The relative standard deviation of the method was less than 2.53% for intra-day assays and 3.23% forÂ inter-day assay, the accuracy of the recovery test ranged from 98.96% to 106.4%. This method was used in comparative qualitative analysisÂ of arbutin and hydroquinone in 16 different raw materials from families Lamiaceae, Ericacaeae, Saxifragaceae, Rosaceae. The content ofÂ arbutin in B. ciliata, B. cordifolia and Ledum palustre was examined for the first time.
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