Morphological and Molecular (SSR) Analysis of Old Apple Cultivars
AbstractInvestigations were made on the morphological traits of the flowers, shoots and fruits of five apple cultivars in the Batul group and four in the SÃ³vÃ¡ri Group, in order to prepare UPOV (Union Internationale pour la Protection des Obtentions VÃ©gÃ©tales) descriptions of these cultivars. Marker analysis involving 12 microsatellite primers was performed to identify 13 genotypes. All the analytical methods indicated that â€˜ZÃ¶ld batulâ€™ could be clearly distinguished from the other â€˜Batulâ€™ selections, suggesting that it is an independent cultivar distantly related to â€˜Batulâ€™. Within the SÃ³vÃ¡ri Group, the cultivars could be clearly distinguished from each other on the basis of morphological and molecular analysis. The variants of â€˜Beregi sÃ³vÃ¡riâ€™ collected in the United Kingdom and in Sub-Carpathia cannot be considered to be the same cultivar. The genotype preserved in the UK National Apple Collection at Brogdale should be considered to be an unknown cultivar. The present examinations demonstrated that a combination of morphological and genetic analyses provides a more reliable identification of apple genotypes than pomological descriptions or genetic analysis alone.
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