A Method for Prediction of Graft Incompatibility in Sweet Cherry
AbstractThis study was carried out on 1-year old trees of â€˜0900 Ziraatâ€™ variety grafted onto â€˜KuÅŸ kiraziâ€™, â€˜Kara idrisâ€™, â€˜Sari idrisâ€™, â€˜MaxMa 14â€™, â€˜MaxMa 60â€™ and â€˜Gisela 5â€™ in order to determine their compatibility. For this purpose, total peroxidase activity was determined by spectrophotomery assay. Taking ground tissue samples were planned three times as before the grafting (beginning), and then 8 and 12 months after grafting. Total peroxidase activities of rootstocks before grafting varied between 10.80 Î”Ag.min. (â€˜KuÅŸ kiraziâ€™) and 7.83 Î”Ag.min. (â€˜Kara idrisâ€™) and were found to be statistically important. The peroxidase activity of â€˜0900 Ziraatâ€™ was 11.07Î”Ag.min. and the closest value occurred in Prunus avium rootstock. The most different values occurred in rootstocks of â€˜Kara idrisâ€™ and â€˜Gisela 5â€™. The results showed that peroxidase activity increased in rootstock and graft scion. This increase had higher values in heterogenetic combinations especially in â€˜0900 Ziraatâ€™/â€˜Gisela 5â€™ and â€˜0900 Ziraatâ€™/â€˜Kara idrisâ€™ grafts. Peroxidase activity was decreased at the 12th month for all combinations. The highest value was obtained from â€˜0900 Ziraatâ€™/â€˜MaxMa 14â€™ combinaion with 29.17 Î”Ag.min. while lowest one was â€˜0900 Ziraatâ€™/â€˜KuÅŸ kiraziâ€™ with 17.39. The findings showed that peroxidase activity could be used as a parameter in early determination of graft incompatibility.
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