Micropropagation of Anubias barteri var. Nana from Shoot Tip Culture and the Analysis of Ploidy Stability

  • Kantamaht KANCHANAPOOM Prince of Songkla University, Faculty of Science, Center for Genomics and Bioinformatics Research, Hat Yai, Songkhla, 90112
  • Panyaros CHUNUI Prince of Songkla University, Faculty of Science, Department of Biology, Plant Biotechnology Research Unit, Hat Yai, Songkhla, 90112
  • Kamnoon KANCHANAPOOM Prince of Songkla University, Faculty of Science, Department of Biology, Plant Biotechnology Research Unit, Hat Yai, Songkhla, 90112

Abstract

Plant regeneration of Anubias barteri var. Nana was achieved through organogenesis in shoot tip cultures. Multiple shoots were induced from cultured shoot tips on a modified MS (Murashige and Skoog, 1962) medium supplemented with BA and kinetin. The maximum green shoot numbers were best obtained on MS medium containing 3 mg/L BA with 5 shoots. Rooting in all regenerated shoots was promoted on MS medium devoid of plant growth regulators or kinetin singly. Acclimatization and survival when transferred to field conditions were shown to be 100% in the regenerated plants. Cytological and flow cytometric analyses of the mother plants and in vitro grown plants derived from 5 years old cultures showed no differences in ploidy level, they were all diploid (2n = 2x = 48) with a 2C peak indicating that ploidy alteration did not occur.

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Author Biographies

Kantamaht KANCHANAPOOM, Prince of Songkla University, Faculty of Science, Center for Genomics and Bioinformatics Research, Hat Yai, Songkhla, 90112
Ph.D. Prince of Songkla University, Faculty of Science, Center for Genomics and Bioinformatics Research, Hat Yai, Songkhla, 90112 Thailand
Panyaros CHUNUI, Prince of Songkla University, Faculty of Science, Department of Biology, Plant Biotechnology Research Unit, Hat Yai, Songkhla, 90112
Prince of Songkla University, Faculty of Science, Department of Biology, Plant Biotechnology Research Unit, Hat Yai, Songkhla, 90112 Thailand.
Kamnoon KANCHANAPOOM, Prince of Songkla University, Faculty of Science, Department of Biology, Plant Biotechnology Research Unit, Hat Yai, Songkhla, 90112
Assoc. Prof. Dr. Department of Biology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112
Published
2012-09-24
How to Cite
KANCHANAPOOM, K., CHUNUI, P., & KANCHANAPOOM, K. (2012). Micropropagation of Anubias barteri var. Nana from Shoot Tip Culture and the Analysis of Ploidy Stability. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 40(2), 148-151. https://doi.org/10.15835/nbha4027520
Section
Research Articles