Hairy Root Induction in Linum mucronatum ssp. mucronatum, an Anti-Tumor Lignans Producing Plant

  • Afsaneh SAMADI University of Urmia, Faculty of Science, Department of Biology, Urmia
  • Jirair CARAPETIAN University of Urmia, Faculty of Science, Department of Biology, Urmia
  • Reza HEIDARI University of Urmia, Faculty of Science, Department of Biology, Urmia
  • Morad JAFARI University of Urmia, Faculty of Agriculture, Department of Agronomy and Plant Breeding, Urmia
  • Abdollah HASSANZADEH GORTTAPEH Agricultural and Natural Resources, Research Center, West Azerbaijan

Abstract

Transgenic hairy root system is a promising source of secondary metabolites in medicinal plants with high pharmaceutical value.For the first time, hairy roots were established in different explants of Linum mucronatum, an anti-cancer agent producing plant, via amikimopine type strain of Agrobacterium rhizogenes, ‘A13’. The percentage of hairy root induction varied from 0 to 60% depended onthe explants and hypocotyl (including cotyledonary node) explants were found to be highly susceptible to A. rhizogenes infection withthe highest (60%) rate of hairy root induction. four different Murashige and Skoog (MS)-based liquid culture media were used for wellestablishment of hairy roots. Hairy root growth medium D (HRGM-D) containing hormone-free MS basal medium with an extra oneday pre-incubation period at 35°C was found to be more efficient for profuse growth (fresh weight; 8500 mg per 25 ml culture medium)of hairy roots. Hairy root system presented in this study may offer a suitable platform for optimization and production of satisfactorylevel of aryltetralin lignans like podophyllotoxin and its derivatives from L. mucronatum.

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Author Biographies

Afsaneh SAMADI, University of Urmia, Faculty of Science, Department of Biology, Urmia
Department of Biology, PhD student
Jirair CARAPETIAN, University of Urmia, Faculty of Science, Department of Biology, Urmia
Department of Biology, Professor
Reza HEIDARI, University of Urmia, Faculty of Science, Department of Biology, Urmia
Department of Biology, Professor
Morad JAFARI, University of Urmia, Faculty of Agriculture, Department of Agronomy and Plant Breeding, Urmia
Department of Agronomy and Plant Breeding, Assistant Professor
Abdollah HASSANZADEH GORTTAPEH, Agricultural and Natural Resources, Research Center, West Azerbaijan
Agricultural and Natural Resources, Research Center; Assistant Professor
Published
2012-05-14
How to Cite
SAMADI, A., CARAPETIAN, J., HEIDARI, R., JAFARI, M., & HASSANZADEH GORTTAPEH, A. (2012). Hairy Root Induction in Linum mucronatum ssp. mucronatum, an Anti-Tumor Lignans Producing Plant. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 40(1), 125-131. https://doi.org/10.15835/nbha4017312
Section
Research Articles