Seasonal Pattern of Flavonoid Content and Related Enzyme Activities in Leaves of Ginkgo biloba L.

  • Shuiyuan CHENG Huanggang Normal University, Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang 438000
  • Feng XU Yangtze University, College of Horticulture and Gardening, Jingzhou 434025
  • Linling LI Huanggang Normal University, Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang 438000
  • Hua CHENG Huanggang Normal University, Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang 438000
  • Weiwei ZHANG Nanjing Forestry University, College of Forest Resources and Environment, Nanjing 210037
Keywords: enzymes; flavonoids; Ginkgo biloba; leaf

Abstract

The contents of flavonoids and other related components, in addition to the activities of related enzymes, such as phenylalanine ammonia-lyase (PAL), peroxidase (POD), chalcone synthase (CHS), and UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT), were determined in Ginkgo biloba L. leaves during leaf development. The results indicated that POD activity increased gradually in the early stages and reached its first activity peak in the beginning of Jul., the second one in late Aug., and the third one (the highest peak) in the middle of Oct. The changes in POD activity were irregular, and its activity was maintained at a low level in the early stages. However, it increased rapidly from the middle of Sep., and reached its highest value in the middle of Oct. Three peaks were obtained for PAL activity. The first peak, with the highest value was in early to middle Jul. The second one, with the lowest value, was in late Aug., and the last one, with a higher value and the longest period, was after the middle of Sep. UFGT activity had a speedy increase in the early stages and continued to increase after May. However, the net increase remained low. CHS activity was enhanced during all stages and showed two obvious peaks. The first time was in early Jul., and the other, with a higher value, was in the middle of Nov. Although PAL activity had a close relationship with the flavonoid content, no notable correlation could be identified between them. A significant correlation between CHS activity and the flavonoid content showed that CHS was one of the key enzymes involved in flavonoid biosynthesis. The relationship between flavonoid content and other related enzymes is discussed in this study.

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Published
2012-05-14
How to Cite
CHENG, S., XU, F., LI, L., CHENG, H., & ZHANG, W. (2012). Seasonal Pattern of Flavonoid Content and Related Enzyme Activities in Leaves of Ginkgo biloba L. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 40(1), 98-106. https://doi.org/10.15835/nbha4017262
Section
Research Articles