Microarray Analysis of the Transcriptome for Bacterial Wilt Resistance in Pepper (Capsicum annuum L.)

  • Jihyun HWANG Pusan National University, Department of Horticultural Bioscience, Miryang, 627- 706
  • Youngmi CHOI Department of Horticultural Bioscience, Pusan National University, Miryang, 627-706,
  • Jumsoon KANG Department of Horticultural Bioscience, Pusan National University, Miryang, 627-706
  • Suntae KIM Department of Plant Bioscience, Pusan National University, Miryang, 627-706
  • Myeongcheoul CHO National Institute of Horticultural & Herbal Science, Rural Development Administration, Suwon, 441-440,
  • Lucica MIHALTE University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, 3-5 Manastur St., 400372
  • Younghoon PARK Pusan National University, Department of Horticultural Bioscience, Miryang 627-706

Abstract

Ralstonia solanacearum causes one of the most common soil-borne vascular diseases of diverse plant species, including many solanaceous crops such as tomato and pepper. The resulting disease, bacterial wilt (BW), is devastating and difficult to control using conventional approaches. The aim of this study was to investigate the differentially expressed genes in pepper root systems in response to infection by R. solanacearum. DNA microarray (Capsicum annuum 135K Microarray v3.0 Gene Expression platform) analyses were performed using a susceptible genotype, ‘Chilbok’, and a resistant genotype, ‘KC350’, at 3 time points (1, 3, and 6 days post inoculation). It has been identified 115 resistance-specific genes (R-response genes) and 109 susceptibility-specific genes (S-response gene), which were up-regulated in 1 genotype, but down-regulated in the other genotype. Gene Ontology (GO) analysis for functional categorization indicated that many R-response genes were related to genes that function in xyloglucan biosynthesis and cell wall organization, while S-response genes were involved in the response to stress and cell death. The expression of genes encoding xyloglucan endotransglycosylase/hydrolase (XTH) and β-galactosidase were verified by real-time RT-PCR at an early time point of R. solanacearum infection. The results supported the idea that rapidly induced XTH expression in ‘KC350’ may play an important role in the restructuring and reinforcement of the cell wall and restrict bacterial movement in xylem vessels. In addition, induced expression of β-galactosidase in R. solanacearum-infected ‘Chilbok’ implied that degradation of the cell wall structure in vascular tissues by β-galactosidase might be an important factor facilitating R. solanacearum invasion of and movement in susceptible host plants.

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Published
2011-11-21
How to Cite
HWANG, J., CHOI, Y., KANG, J., KIM, S., CHO, M., MIHALTE, L., & PARK, Y. (2011). Microarray Analysis of the Transcriptome for Bacterial Wilt Resistance in Pepper (Capsicum annuum L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 39(2), 49-57. https://doi.org/10.15835/nbha3926820
Section
Research Articles