Two Novel Alliin Lyase (Alliinase) Genes from Twisted-Leaf Garlic (Allium obliquum) and Mountain Garlic (Allium senescens var. montanum)

  • Bogdan DRUGĂ Institute of Biological Research, 48 Republicii Street, 400015, Cluj-Napoca
  • Dana ŞUTEU Institute of Biological Research, 48 Republicii Street, 400015, Cluj-Napoca
  • Oana ROSCA-CASIAN BabeÅŸ-Bolyai University, Faculty of Biology and Geology, „Alexandru Borza” Botanical Garden, 42 Republicii Street, 400015, Cluj-Napoca
  • Marcel PÂRVU BabeÅŸ-Bolyai University, Faculty of Biology and Geology, „Alexandru Borza” Botanical Garden, 42 Republicii Street, 400015, Cluj-Napoca
  • Nicolae DRAGOŞ Institute of Biological Research, 48 Republicii Street, 400015, Cluj-Napoca

Abstract

Alliinase (Alliin lyase EC 4.4.1.4), a pyridoxal phosphate-dependent lyase, represents one of the major protein components of Allium species. The enzyme is a homodimeric glycoprotein and catalyzes the synthesis of allicin (diallyl thiosulfinate, a biologically active compound), pyruvate, and ammonia starting from the specific non-protein sulfur-containing amino acid alliin ((+S)-allyl-L-cysteine sulfoxide). Using newly developed specific primers two new alliinase genes from Allium obliquum and Allium senescens ssp. montanum were amplified and sequenced, as well as their homologs, from Allium fistulosum and Allium schoeonoprasum. The G+C content of the alliinase region ranges between that of other dicot plants and that reported in monocot cereal plants, in all four species. Investigations of gene expression revealed a significantly higher enzyme expression level in bulbs than in leaves in all four taxa. The deduced alliinase sequences displayed a high variability among different species, since the lowest sequence similarity was found to be 55.5% between Allium senescens var. montanum and Allium cepa, while the highest similarity is 77.5%, between Allium senescens var. montanum and Allium fistulosum. Leucine is the most common amino acid in all four alliinases, while cysteine is also more frequent that in other enzymes, suggesting a high stability of the molecules due to the possible disulfide bonds.

Metrics

Metrics Loading ...

Author Biographies

Bogdan DRUGĂ, Institute of Biological Research, 48 Republicii Street, 400015, Cluj-Napoca
Dept. of Taxonomy and Ecology, Senior Researcher
Dana ŞUTEU, Institute of Biological Research, 48 Republicii Street, 400015, Cluj-Napoca
Dept. of Taxonomy and Ecology, Senior Researcher
Oana ROSCA-CASIAN, Babeş-Bolyai University, Faculty of Biology and Geology, „Alexandru Borza” Botanical Garden, 42 Republicii Street, 400015, Cluj-Napoca
Researcher
Marcel PÂRVU, BabeÅŸ-Bolyai University, Faculty of Biology and Geology, „Alexandru Borza” Botanical Garden, 42 Republicii Street, 400015, Cluj-Napoca
Associate
Nicolae DRAGOŞ, Institute of Biological Research, 48 Republicii Street, 400015, Cluj-Napoca
Dept. of Taxonomy and Ecology, Senior Researcher I
Published
2011-11-21
How to Cite
DRUGĂ, B., ŞUTEU, D., ROSCA-CASIAN, O., PÂRVU, M., & DRAGOŞ, N. (2011). Two Novel Alliin Lyase (Alliinase) Genes from Twisted-Leaf Garlic (Allium obliquum) and Mountain Garlic (Allium senescens var. montanum). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 39(2), 293-298. https://doi.org/10.15835/nbha3926355
Section
Research Articles

Most read articles by the same author(s)