Screening of Biotechnological Parameters for Fructofuranosidases Production by a Newly Isolated Fungal Strain Using Plackett-Burman Design

  • Camelia NEAGU BONCIU Dunarea de Jos University, Food Science and Engineering Faculty, 111 Domneasca Street, 800201, Galati
  • Oana CONSTANTIN Dunarea de Jos Dunarea de Jos University, Food Science and Engineering Faculty, 111 Domneasca Street, 800201, GalatiUniversity
  • Gabriela Elena BAHRIM University “Dunarea de Jos”, Faculty of Food Science and Engineering, Bioengineering Department, 111 Domneasca Str. , GalaÅ£i, Romania

Abstract

Previously some fungal strains with good inulinase potential biosynthesis were screening and the most performing strain is the newly fungal strain, Rhizoctonia spp. coded I-PN4. In this study, the Plackett Burman design was used to identify the effect of process parameters upon fructofuranosidases (inulinase and invertase) production with new selected fungal strain. The agitation rate, yeast extract, buckwheat flour and K2HPO4 concentration from fermentative medium composition, were determined as the most influential variables that have positive effects on fructofuranosidases biosynthesis. Temperature has a negative effect on both invertase and inulinase biosynthesis yield. In optimal established fermentative conditions, the maximum yields for extracellular inulinase was 6.2198 UI and 4.7664 UI for invertase respectively. The linear model fits well for variable screening and is globally significant, as R2 is 88.36% for inulinase production and 84.24% for invertase production respectively.

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Published
2011-11-21
How to Cite
NEAGU BONCIU, C., CONSTANTIN, O., & BAHRIM, G. E. (2011). Screening of Biotechnological Parameters for Fructofuranosidases Production by a Newly Isolated Fungal Strain Using Plackett-Burman Design. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 39(2), 271-275. https://doi.org/10.15835/nbha3926323
Section
Research Articles