Identification, Characterization, and Expression Analysis of the TaNF-YB3 Gene from Triticum aestivum

  • Xiuchun DONG Shandong Agricultural University, College of Life Sciences, Taian, Shandong 271018
  • Zhiyuan CHENG Shandong Agricultural University, College of Life Sciences, Taian, Shandong 271018
  • Shuhan CHENG Shandong Agricultural University, College of Information Science and Engineering, Taian, Shandong 271018
  • Feng XU Yangtze University, College of Horticulture and Gardening, Jingzhou 434025
  • Yanrong AN Shandong Agricultural University, College of Life Sciences, Taian, Shandong 271018
  • Xizuo BIAN Shandong Agricultural University, College of Life Sciences, Taian, Shandong 271018
  • Ting CHEN Shandong Agricultural University, College of Life Sciences, Taian, Shandong 271018

Abstract

A full-length cDNA encoding a nuclear factor-YB (NF-YB)/HAP3/CCAAT binding factor-A (CBF-A) subunit of a CCAAT-box binding complex, designated as TaNF-YB3 was isolated from Triticum aestivum. Sequence analysis indicated that the full-length cDNA was 809 bp long, including an open reading frame (ORF) of 597 bp, which encoded a deduced polypeptide of 199 amino acids and is located in chromosome 3D. The deduced protein contained conserved structural domains and showed high identity to other plant NF-YBs. TaNF-YB3 was expressed in various organs, especially in the leaves and stamens; it was also regulated by salt, mannitol, abscisic acid, wounding, and cold. Moreover, TaNF-YB3 was down-regulated by short days and vernalization, and sensitive to the transfer of day length. It was mainly induced by light and exhibited a similar diurnal rhythmic expression pattern with the CCT-domain family gene VRN2 (TaZCCT1 and TaZCCT2), but not with CO (WCO1 and TaHd1). Overall, the results suggested that TaNF-YB3, aside from having a role in regulating day length and vernalization responses, might integrate signals from other environmental stresses to perform its functions in winter wheat adaptability and development.

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Author Biography

Feng XU, Yangtze University, College of Horticulture and Gardening, Jingzhou 434025
College of Horticulture and Gardening
Published
2011-11-21
How to Cite
DONG, X., CHENG, Z., CHENG, S., XU, F., AN, Y., BIAN, X., & CHEN, T. (2011). Identification, Characterization, and Expression Analysis of the TaNF-YB3 Gene from Triticum aestivum. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 39(2), 254-263. https://doi.org/10.15835/nbha3926083
Section
Research Articles