In vitro Flowering of Shoots Regenerated from Cultured Nodal Explants

  • Kantamaht KANCHANAPOOM Prince of Songkla University, Faculty of Science, Center for Genomics and Bioinformatics Research, Hat Yai, Songkhla, 90112
  • Suttinee JINGJIT Prince of Songkla University, Center for Genomics and Bioinformatics Research, Hat Yai, Songkhla, 90112
  • Kamnoon KANCHANAPOOM Prince of Songkla University, Faculty of Science, Department of Biology, Plant Biotechnology Research Unit, Hat Yai, Songkhla, 90112

Abstract

A protocol for the regeneration of Gypsophila paniculata L. using nodal explants from 2-month-old field grown plants was established. The induction of multiple shoots was best obtained on Murashige and Skoog (MS) medium supplemented with 13.3 μM BA. Callus growth was observed on MS medium containing 44.3 μM BA. Calluses were transferred to MS medium supplemented with 2, 4-D (4.5, 13.5, 22.6 μM), NAA (5.3, 16.1, 26.8 μM) or BA (4.4, 13.3, 22.1 μM) for 2 months to induce shoot formation. After 6 weeks of initial culture, multiple shoots were regenerated from calluses cultured on MS medium supplemented with 13.3 μM BA. All regenerated shoots produced roots on 16.1 μM NAA containing MS medium within 4 weeks. Rooted plantlets were hardened and established in pots at 100% survival. For induction of in vitro flowering, regenerated shoots could be induced to flower efficiently when cultured on MS medium containing 13.3 μM BA and 50 g/l sucrose.

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Author Biographies

Kantamaht KANCHANAPOOM, Prince of Songkla University, Faculty of Science, Center for Genomics and Bioinformatics Research, Hat Yai, Songkhla, 90112
Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112
Suttinee JINGJIT, Prince of Songkla University, Center for Genomics and Bioinformatics Research, Hat Yai, Songkhla, 90112
Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112
Kamnoon KANCHANAPOOM, Prince of Songkla University, Faculty of Science, Department of Biology, Plant Biotechnology Research Unit, Hat Yai, Songkhla, 90112
Assoc. Prof. Dr. Department of Biology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112
Published
2011-05-30
How to Cite
KANCHANAPOOM, K., JINGJIT, S., & KANCHANAPOOM, K. (2011). In vitro Flowering of Shoots Regenerated from Cultured Nodal Explants. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 39(1), 84-87. https://doi.org/10.15835/nbha3914994
Section
Research Articles