Regeneration of Blackgram (Vigna mungo L.) on Changes of Hormonal Condition

  • Shariful Alam MONY Department of Biotechnology, Bangladesh Agricultural University
  • Md. Shahidul HAQUE Department of Biotechnology, Bangladesh Agricultural University
  • Md. Mahabub ALAM Department of Agronomy, Sher-e-Bangla Agricultural University
  • Mirza HASANUZZAMAN Sher-e-Bangla Agricultural University, Department of Agronomy, Faculty of Agriculture, Dhaka-1207
  • Kamrun NAHAR Department of Agricultural Botany, Sher-e-Bangla Agricultural University

Abstract

The study comprised of experiments for shoot regeneration and plantlet formation from cotyledonary node of Vigna mungo by culturing them on low concentration of BAP followed by transfer to hormone free MS medium. Cotyledonary node explants were cultured on different concentrations of BAP (0, 1, 2.5 and 50 mg l-1). Shoot regeneration occurred from cotyledonary nodes irrespective of the presence or absence of BAP in the medium. However, culture of cotyledonary node explants for 10 days on medium containing 1.0 mg l-1 BAP followed by transfer to hormone free medium gave higher number of shoots (9.33/explant) compared to culture of the explants on hormone free medium for 15 days followed by transfer to medium containing 1.0 mg l-1 BAP (8.33/explants). The regenerated shoots were transferred to rooting medium supplemented with different concentrations of IBA and NAA. The high frequency (100.0%) of rooting was observed with MS medium supplemented with 0.5 mg l-1 IBA. The rooted plants were transferred to pots for hardening.

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Author Biography

Mirza HASANUZZAMAN, Sher-e-Bangla Agricultural University, Department of Agronomy, Faculty of Agriculture, Dhaka-1207
Assistant Professor
Published
2010-12-05
How to Cite
MONY, S. A., HAQUE, M. S., ALAM, M. M., HASANUZZAMAN, M., & NAHAR, K. (2010). Regeneration of Blackgram (Vigna mungo L.) on Changes of Hormonal Condition. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38(3), 140-145. https://doi.org/10.15835/nbha3834744
Section
Research Articles