Changes in Botrytis cinerea Conidia Caused by Berberis vulgaris Extract

  • Marcel PARVU Department of Biology, Faculty of Biology and Geology, Babes-Bolyai University, 42 Republicii Street, 400015 Cluj-Napoca
  • Alina Elena PARVU Department of Pathophysiology, Faculty of Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Victor Babes St., 400012 Cluj-Napoca
  • Constantin CRACIUN Babes-Bolyai University, Center for Electronic Microscopy, 5-7 Clinicilor Street, 400006 Cluj-Napoca
  • Lucian BARBU-TUDORAN Center for Electron Microscopy, Babes-Bolyai University, 5-7 Clinicilor Street, 400006 Cluj-Napoca
  • Laurian VLASE Iuliu Hatieganu University of Medicine and Pharmacy, Faculty of Pharmacy, 12 Ion Creanga Street, 400010 Cluj-Napoca
  • Mircea TAMAS Iuliu Hatieganu University of Medicine and Pharmacy, Faculty of Pharmacy, 12 Ion Creanga Street, 400010 Cluj-Napoca
  • Oana ROSCA-CASIAN BabeÅŸ-Bolyai University, Faculty of Biology and Geology, „Alexandru Borza” Botanical Garden, 42 Republicii Street, 400015, Cluj-Napoca
  • Ovidiu PERSECA Institute of Public Health Cluj-Napoca, 6 Louis Pasteur Street, 400349 Cluj-Napoca
  • Ana-Maria MOLNAR Institute of Public Health Cluj-Napoca, 6 Louis Pasteur Street, 400349 Cluj-Napoca

Abstract

Testing plant extracts for controlling fungal diseases is a main biocontrol method. More interesting is to see what happens to the fungus treated with the plant extract. Therefore, the aim of the study was to evaluate the antifungal activity of Berberis vulgaris extract on Botrytis cinerea and to examine the ultrastructural changes in B. cinerea conidia caused by the minimum inhibitory concentration (MIC), using SEM and TEM. The antifungal activity of B. vulgaris bark extract was investigated using agar dilution method, and compared to that of berberine. Fluconazole was used as the positive antimycotic control. It was found that (1) B. vulgaris bark extract had significant antifungal activity against B. cinerea, and its effect was stronger than that of pure berberine. It was also noted that (2)B. vulgaris MIC caused severe structural changes of the conidia, comparable with berberine MIC effect; therefore (3) B. vulgaris bark extract might be recommended to be tested as a biocontrol agent against B. cinerea.

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Published
2010-12-05
How to Cite
PARVU, M., PARVU, A. E., CRACIUN, C., BARBU-TUDORAN, L., VLASE, L., TAMAS, M., ROSCA-CASIAN, O., PERSECA, O., & MOLNAR, A.-M. (2010). Changes in Botrytis cinerea Conidia Caused by Berberis vulgaris Extract. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38(3), 15-20. https://doi.org/10.15835/nbha3834741
Section
Research Articles