In vitro Propagation of Adenium obesum (Forssk.) Roem. and Schult.

  • Kantamaht KANCHANAPOOM Prince of Songkla University, Faculty of Science, Center for Genomics and Bioinformatics Research, Hat Yai, Songkhla, 90112
  • Sunisa SUNHEEM Prince of Songkla University, Faculty of Science, Center for Genomics and Bioinformatics Research, Hat Yai, Songkhla, 90112
  • Kamnoon KANCHANAPOOM Prince of Songkla University, Faculty of Science, Department of Biology, Plant Biotechnology Research Unit, Hat Yai, Songkhla, 90112

Abstract

An in vitro protocol using shoot tip explants from seedling of Adenium obesum (Forssk.) Roem. and Schult. was developed. Explants were cultured on Murashige and Skoog (MS) medium supplemented with various combinations of NAA and BA. The most effective medium for shoot proliferation at a high frequency of 5.201.10 shoots per explant consisted of 22.2 ?M BA. High rooting and survival were achieved using MS medium supplemented with 0.3% activated charcoal and without any growth regulators. Rooted plants were successfully acclimatized to greenhouse conditions. This study showed that A. obesum could be micropropagated by utilizing multiple shoots derived from seedling shoot tips. A flow cytometric analysis for DNA content revealed no differences among the micropropagated plants and the in vivo natural grown plants. The resulting 2C DNA value (8.350.039 pg) of this species was estimated for the first time.

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Author Biographies

Kantamaht KANCHANAPOOM, Prince of Songkla University, Faculty of Science, Center for Genomics and Bioinformatics Research, Hat Yai, Songkhla, 90112
Dr. Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112
Sunisa SUNHEEM, Prince of Songkla University, Faculty of Science, Center for Genomics and Bioinformatics Research, Hat Yai, Songkhla, 90112
Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112
Kamnoon KANCHANAPOOM, Prince of Songkla University, Faculty of Science, Department of Biology, Plant Biotechnology Research Unit, Hat Yai, Songkhla, 90112
Assoc. Prof. Dr. Department of Biology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112
Published
2010-12-05
How to Cite
KANCHANAPOOM, K., SUNHEEM, S., & KANCHANAPOOM, K. (2010). In vitro Propagation of Adenium obesum (Forssk.) Roem. and Schult. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38(3), 209-213. https://doi.org/10.15835/nbha3834604
Section
Research Articles