Characterisation of Newly Polar Psychrotrophic Streptomycetes Isolates from Polar Soils with Cold Adapted Bioremediation Potential

  • Mihaela COTARLET niversity “Dunarea de Jos”, Faculty of Food Science and Engineering, Bioengineering Department, 111 Domneasca Str., GalaÅ£i
  • Gabriela Elena BAHRIM University “Dunarea de Jos”, Faculty of Food Science and Engineering, Bioengineering Department, 111 Domneasca Str. , GalaÅ£i, Romania
  • Teodor Gheorghe NEGOITA Romanian Polar Research Institute
  • Peter STOUGAARD University of Copenhagen

Abstract

Novel polar streptomycetes coded 4 Alga, P2C4 and MIUG 12P, isolated from soil and vegetation samples prelevated from East Antarctica, were genetically and biochemically characterized in order to set up the phylogeny and to identify the capability to grow at lower temperature and to generate amylase and protease cold-active. The 16S rRNA gene sequence of isolate coded 4 Alga was 100% identical to sequences of Streptomyces sp. isolates from Norway and from Solomon Islands, which also were the closest relatives of P2C4 (99.53%) and MIUG 12P (99.53%) isolates. The data emphasised the skills of the strains Streptomyces 4 Alga, Streptomyces P2C4 and Streptomyces MIUG 12P to biosynthesis cold-adapted amylases and proteases suitable in bioconversion processes at low temperature. These results are quit valuable, because only few streptomycetes, particularly psychrotolerant ones, have so far been explored for their enzymatic potential in cold conditions.

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Published
2010-06-15
How to Cite
COTARLET, M., BAHRIM, G. E., NEGOITA, T. G., & STOUGAARD, P. (2010). Characterisation of Newly Polar Psychrotrophic Streptomycetes Isolates from Polar Soils with Cold Adapted Bioremediation Potential. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38(1), 61-65. https://doi.org/10.15835/nbha3814595
Section
Research Articles