Use of Phenotypic and MAS Selection Based on Bulk Segregant Analysis to Reveal the Genetic Variability Induced by Artificial Hybridization in Apple

Authors

  • Radu E. SESTRAS University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, 3-5 Mănăștur St., 400372 (RO)
  • Doru PAMFIL University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, 3-5 Mănăștur St., 400372 (RO)
  • Marin ARDELEAN University of Agricultural Science and Veterinary Medicine, 3-5 Mănăștur St., 400372 Cluj-Napoca (RO)
  • Constantin BOTEZ University of Agricultural Science and Veterinary Medicine, 3-5 Mănăștur St., 400372 Cluj-Napoca (RO)
  • Adriana F. SESTRAS Fruit Research Station, 3-5 Horticultorilor St., Cluj-Napoca 400454 (RO)
  • Ioana MITRE University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, 3-5 Mănăștur St., 400372 (RO)
  • Catalina DAN University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, 3-5 Mănăștur St., 400372 (RO)
  • Lucica MIHALTE University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, 3-5 Mănăștur St., 400372 (RO)

DOI:

https://doi.org/10.15835/nbha3713135

Keywords:

apple hybrids, breeding, BSA technique, Marker Assisted Selection

Abstract

Phenotypic selection in apple seedling populations (F1), derived from semidiallel hybridizations between genitors with different peculiarities (Florina and Liberty, resistant to apple scab attack; Starkrimson and Golden spur, with spur ideotype) was completed with Marker Assisted Selection (MAS), based on Bulk Segregant Analysis (BSA) technique. Molecular analyses performing by Bulk Segregant Analysis were intended to identify markers for scab resistance, powdery mildew resistance and architectural tree structure (ideotype) with bulks of five to six plants per group and testing the bulks with RAPD polymorphic primers. The polymorphic bands between the bulks were mostly associated to apple scab resistance genes. Concerning powdery mildew resistance and architectural tree structure analysis the results are inconclusive. Although it seems that the primer 54 showed a polymorphic band in a resistant to powdery mildew hybrid, which is missing in the sensitive hybrids, the result is only apparently favourable since this band is present in both parental forms (Starkrimson, tolerant to powdery mildew and Liberty, relative susceptible). Molecular profiles of the standard ideotype and spur ideotype, resulted in hybrids originated in standard parental form (Florina) and spur parental form (Golden spur), were usually monomorphic. Genetic association among the parental molecular polymorphism for scab resistance and the other two important traits (powdery mildew resistance and ideotype), was not conclusive.

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Published

2009-06-30

How to Cite

SESTRAS, R. E., PAMFIL, D., ARDELEAN, M., BOTEZ, C., SESTRAS, A. F., MITRE, I., DAN, C., & MIHALTE, L. (2009). Use of Phenotypic and MAS Selection Based on Bulk Segregant Analysis to Reveal the Genetic Variability Induced by Artificial Hybridization in Apple. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 37(1), 273–277. https://doi.org/10.15835/nbha3713135

Issue

Vol. 37 No. 1 (2009)

Section

Research Articles
CITATION
DOI: 10.15835/nbha3713135

License

Copyright (c) 2009 Radu E. SESTRAS, Doru PAMFIL, Marin ARDELEAN, Constantin BOTEZ, Adriana F. SESTRAS, Ioana MITRE, Catalina DAN, Lucica MIHALTE

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

License:

© Articles by the authors; licensee UASVM and SHST, Cluj-Napoca, Romania. Papers published in the journal Notulae Botanicae Horti Agrobotanici Cluj-Napoca are open access distributed under the terms and conditions of the Creative Commons Attribution License (http://creativecommons.org/licenses).

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