Optimization of DNA Extraction from Seeds of Sorghum sudanense (Piper) Stapf.
Keywords:Sorghum sudanense, DNA extraction, Moller’s method, optimization
Sudan Grass (Sorghum sudanense (Piper) Stapf.) is a fine annual forage of Gramineae, Sorghum Genus that thrives in warm natural environment, with strong resistance against drought and barren soil, high yield, strong regeneration capability. DNA of the grass seeds was extracted by optimized Moller’s method. The extraction of DNA is a premise for identifying Sorghum sudanense from malignant weed seeds such as Sorghum halepense by molecular biological methods. The effects of NH4Ac, different seed pretreatments, the ratio of seed powder to TES extraction solution volume (solid-liquid ratio), pyrolysis time, PVP (polyvinyl pyrrolidone) on the DNA extraction were studied. Based on results above, an optimized method for DNA extraction from Sorghum sudanense seeds was established. The results showed that NH4Ac had no effect on DNA extraction from Sorghum sudanense seeds. Soaking seeds in warm water 20 min before grinding, 1:10 for solid-liquid ratio, pyrolysing for 60 min and 1.5% (w/v) PVP in extraction solution could promote the quantity and purity of DNA, respectively.
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