Marker Assisted Selection for Septoria tritici Resistance in Wheat Dihaploid Lines
Keywords:SSR markers, wheat dihaploid lines, resistant genes to Septoria tritici
The selection with co-dominant SSR markers existent on the market, accomplished in dihaploid wheat lines, offers the opportunity to rapidly stabilize the selected resistant individuals into homozygous estate. As biological material there were used 21 resistant and two sensitive dihaploid lines. All dihaploid lines tested, with field resistance to Septoria tritici leaf blotch, expressed at least once such a resistant gene. The most frequently there were encountered in the tested material the Stb 7 and Stb 8 leaf blotch resistant genes. The size of amplification products specific for SSR markers linked with Stb 7 and Stb 8 resistant genes were similar to those obtained by Adhikari et al. (2004) but the size of amplification products linked with Stb 2 resistant genes were quite different, probably due to the polymorphism that characterizes microsatellite markers at the allelic level. The Stb3, Stb 4 and Stb 6 resistant genes were totally absent. The results obtained for Stb 1 and Stb 5 were inconclusive.
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