The genetic applications of plant cell and tissue culture techniques: Essential tools for genetic manipulation and crop improvement

Uthman B. ALGOPISHI; Ahmed M. ABDELGHFFAR; Nora M. AL ABOUD; Fatmah A. SAFHI; Eman FAYAD; Khadiga ALHARBI; Nada I. ALJWAIZEA; Abdallah A. HASSANIN

doi:10.15835/nbha53314718

Authors

Uthman B. ALGOPISHI King Khalid University, College of Science, Department of Biology, Abha, 61413 (SA)
Ahmed M. ABDELGHFFAR Zagazig University, Faculty of Agriculture, Genetics Department, Zagazig, 44511 (EG)
Nora M. AL ABOUD Umm Al-Qura University, Faculty of Science, Department of Biology, Makkah, 24372 (SA)
Fatmah A. SAFHI Princess Nourah bint Abdulrahman University, College of Science, Department of Biology, Riyadh, 11671 (SA)
Eman FAYAD Taif University, College of Sciences, Department of Biotechnology, Taif, 21944 (SA)
Khadiga ALHARBI Princess Nourah bint Abdulrahman University, College of Science, Department of Biology, Riyadh, 11671 (SA)
Nada I. ALJWAIZEA Princess Nourah bint Abdulrahman University, College of Science, Department of Biology, Riyadh, 11671 (SA)
Abdallah A. HASSANIN Zagazig University, Faculty of Agriculture, Genetics Department, Zagazig, 44511 (EG)

DOI:

https://doi.org/10.15835/nbha53314718

Keywords:

embryo rescue, genetic engineering, genetic variation, micropropagation, plant cell culture, plant tissue culture, virus elimination

Abstract

Plant cell and tissue culture techniques are fundamental to contemporary plant science, providing essential tools for genetic manipulation and crop improvement. This review examines the primary applications of these techniques, emphasizing their role in advancing fundamental plant biology and developing novel agricultural strategies. Micropropagation is a technique that enables the rapid and efficient asexual propagation of superior genotypes, which is crucial for conserving and disseminating valuable plant material. Furthermore, meristem culture effectively eradicates viruses from infected plants, ensuring the production of disease-free planting stock. Tissue culture techniques are also instrumental in generating genetic variability through somaclonal variation, in vitro mutagenesis, and in vitro selection. These methods provide a substantial source of genetic diversity, facilitating the development of new plant varieties with advantageous characteristics. Beyond generating variation, tissue culture is indispensable for genetic engineering, allowing for the stable integration of exogenous DNA into plant cells to produce transgenic plants with novel traits. Embryo rescue is another significant application. It overcomes challenges in seed development and enabling successful hybridization between otherwise incompatible plant species. By salvaging immature embryos and culturing them to maturity, this technique allows for the creation of hybrid plants possessing desirable trait combinations that would be unattainable through conventional breeding. In conclusion, these techniques have profoundly transformed plant science, offering diverse applications for genetic manipulation, crop improvement, and basic research. By enabling precise control over plant development at the cellular and tissue levels, these techniques are critical for developing improved crops with enhanced yield, nutritional quality, and resilience to environmental stressors.

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