Improving strawberry fruit quality through breeding: cultivar performance and biochemical diversity
DOI:
https://doi.org/10.15835/nbha53314704Keywords:
biochemical diversity, cultivar evaluation, fruit quality, Fragaria × ananassa, pedigree analysis, yield performanceAbstract
Strawberry (Fragaria × ananassa Duch.) breeding has a long tradition worldwide, driven by the need to combine high yields with superior fruit quality and resilience to environmental challenges. At the Research Institute for Fruit Growing Pitești, Romania, breeding activities began in the 1980s and expanded significantly in the 21st century. The first registered cultivar, ‘Premial’, released in 1989, remains the dominant variety grown in Romania. The program is based on traditional cross-pollination and recurrent field selection, in which new seedlings are rapidly evaluated and the best individuals are advanced as parents for the next generation. Breeding objectives target disease resistance, early and consistent yields, and plant architecture that facilitates harvesting. Market preferences emphasize uniform fruit size, colour and shape, while consumers especially value flavour. To provide a reference for future breeding, this study analyzed seven cultivars released between 1980 and 2019. Pedigree data were examined to identify the most influential parental genotypes, and the genetic contribution of ancestral parents was estimated for each cultivar. Agronomic performance, yield and fruit quality were also evaluated. Among the tested cultivars, ‘Ireal’ and ‘Sarom’ showed the best overall performance for yield and fruit quality, while ‘Coral’ was outstanding for sweetness (11.50 °Brix). ‘Sarom’ also exhibited large fruit size (24.20 g/fruit) and high firmness (37.73 N), making it particularly suitable for storage and commercial distribution. These findings highlight the potential of newly developed cultivars, especially ‘Sarom’ and ‘Ireal’, for cultivation under local conditions and as valuable resources for future breeding programs.
References
Akiyama Y, Yamamoto Y, Ohmido N, Ohshima M, Fukui K (2001). Estimation of the nuclear DNA content of strawberries (Fragaria spp.) compared with Arabidopsis thaliana by using dual-step flow cytometry. Cytologia 66(4):431-436. https://doi.org/10.1508/cytologia.66.431
Antunes LEC, Ristow NC, Krolow ACR, Carpenedo S, Reisser Júnior C (2010). Yield and quality of strawberry cultivars. Horticultura Brasileira 28(2):222-226. https://doi.org/10.1590/S0102-05362010000200015
Bernardo R (2020). Reinventing quantitative genetics for plant breeding: something old, something new, something borrowed, something BLUE. Heredity 125(6):375-385. https://doi.org/10.1038/s41437-020-0312-1
Braniște N, Budan S, Butac M, Militaru M (2007). Soiuri de pomi arbuşti fructiferi şi căpşun, create în România [Varieties of fruit trees, shrubs and strawberries, created in Romania]. Paralela 45, Sibiu, Romania p 476.
Cervantes L, Ariza MT, Miranda L, Lozano D, Medina JJ, Soria C, Martínez-Ferri E (2020). Stability of fruit quality traits of different strawberry varieties under variable environmental conditions. Agronomy 10(9):1242. https://doi.org/10.3390/agronomy10091242
Chandler CK, Folta K, Dale A, Whitaker VM, Herrington M (2012). Strawberry. In: Badenes M, Byrne D (Eds). Fruit breeding. handbook of plant breeding, vol 8. Springer, Boston, MA pp 305-325. https://doi.org/10.1007/978-1-4419-0763-9_9
Cocco C, Magnani S, Maltoni ML, Quacquarelli I, Cacchi M, Antunes LE, D’Antuono LF, Faedi W, Baruzzi G (2015). Effects of site and genotype on strawberry fruits quality traits and bioactive compounds. Journal of Berry Research 5(3):145-155. https://doi.org/10.3233/JBR-150098
Cociu V, Botu I, Şerboiu L (1999). Progrese în ameliorarea plantelor horticole din România [Progress in horticultural plant breeding in Romania]. Ceres, Bucharest, Romania p 215.
Costa BM, de Lima JM, de Souza HR, dos Santos MF, Nerbass FR, Kretzschmar AA, Rufato L (2025). New strawberry genotypes adapted for the Southern Plateau of Santa Catarina: a study of yield and fruits quality. Discover Plants 2:192. https://doi.org/10.1007/s44372-025-00279-0
Darrow GM (1966). The strawberry. History, breeding and physiology. (1st ed). Holt, Rinehart & Winston, New York pp 447. https://www.cabidigitallibrary.org/doi/full/10.5555/19681601719
Faedi W, Baruzzi G (2016). Strawberry breeding. In: Husaini AM (Ed). Strawberry: growth, development and diseases. Cabi, Wallingford, UK pp 26-46. https://doi.org/10.1079/9781780646633.0026
FAOSTAT (2025). Food agriculture organization. Retrieved in 2025 July 02 https://www.fao.org/faostat/en/#data/RL
Feldmann MJ, Pincot DD, Cole GS, Knapp SJ (2024). Genetic gains underpinning a little-known strawberry green revolution. Nature Communications 15(1):2468. https://doi.org/10.1038/s41467-024-46421-6
Giovanetti G (2024). A new strawberry (Fragaria x ananassa) breeding program to advance critical steps and obtain new resilient and high-quality cultivars. PhD Thesis. Universita' Politecnica delle Marche.
Hancock JF, Luby JJ (1993). Genetic resources at our doorstep: The wild strawberries. Bio Science 43(3):141-147. https://doi.org/10.2307/1312017
Hera O (2024). The origin of Romanian blueberry cultivars. Scientific Papers. Series B. Horticulture 68(1):45-51. https://horticulturejournal.usamv.ro/index.php/scientific-papers/issues?id=1469
Hera O, Sturzeanu M, Vijan LE (2025). Intraspecific hybridization and heritability of biometric and biochemical traits in F1 blueberry (Vaccinium corymbosum L.) hybrids. Horticulturae 11(6):630. https://doi.org/10.3390/horticulturae11060630
Hummer KE, Hancock J (2009). Strawberry genomics: botanical history, cultivation, traditional breeding, and new technologies. In: Folta KM, Gardiner SE (Eds) Genetics and genomics of Rosaceae. plant genetics and genomics: Crops and models, vol 6. Springer, New York, NY. pp 413-435. https://doi.org/10.1007/978-0-387-77491-6_20
Khoury CK, Brush S, Costich DE, Curry HA, De Haan S, Engels JM, ... Thormann I (2022). Crop genetic erosion: understanding and responding to loss of crop diversity. New Phytologist 233(1):84-118. https://doi.org/10.1111/nph.1773
Lado J, Vicente E, Manzzioni A, Ares G (2010). Application of a check‐all‐that‐apply question for the evaluation of strawberry cultivars from a breeding program. Journal of the Science of Food and Agriculture 90(13):2268-2275. https://doi.org/10.1002/jsfa.4081
Lee J, Durst RW, Wrolstad RE, Barnes, KW, Eisele, T, Giusti MM, ... Wightman JD (2005). Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: Collaborative study. Journal of AOAC International 88(5):1269-1278. https://doi.org/10.1093/jaoac/88.5.1269
Lyzhin AS, Luk’yanchuk IV (2024). Study of a genetic collection of strawberry (Fragaria L.) for resistance to powdery mildew. Vavilov Journal of Genetics and Breeding 28(2):166. https://doi.org/10.18699/vjgb-24-19
Marta AE, Camadro EL, Díaz-Ricci JC, Castagnaro AP (2004). Breeding barriers between the cultivated strawberry, Fragaria × ananassa, and related wild germplasm. Euphytica 136:139-150. https://doi.org/10.1023/B:EUPH.0000030665.95757.76
Matić P, Sabljić M, Jakobek L (2017). Validation of spectrophotometric methods for the determination of total polyphenol and total flavonoid content, Journal of AOAC International 100(6):1795-1803. https://doi.org/10.5740/jaoacint.17-0066
Mazilu IE, Vîjan LE, Cosmulescu S (2022). The influence of harvest moment and cultivar on variability of some chemical constituents and antiradical activity of dehydrated chokeberry pomace. Horticulturae 8(6):544. https://doi.org/10.3390/horticulturae8060544
Mezzetti B (2013). Breeding and biotechnology for improving the nutritional quality of strawberry. Journal of Berry Research 3(3):127-133. https://doi.org/10.3233/JBR-130053
Morrow EB, Darrow GM (1941). Inheritance of some characteristics in strawberry varieties. Proceedings of the American Society for Horticultural Sciences 39:262-268.
Morrow EB, Darrow GM (1952). Effects of limited inbreeding in strawberries. Proceedings of the American Society for Horticultural Sciences 59:269-276.
Neri D, Baruzzi G, Massetani F, Faedi W (2012). Strawberry production in forced and protected culture in Europe as a response to climate change. Canadian journal of plant science 92(6):1021-1036. https://doi.org/10.4141/cjps2011-276
Oliver P, Cicerale S, Pang E, Keast R (2018). Identifying key flavors in strawberries driving liking via internal and external preference mapping. Journal of Food Science 83(4):1073-1083. https://doi.org/10.1111/1750-3841.14109
Pincot DDA, Ledda M, Feldmann MJ, Hardigan MA, Poorten TJ, Runcie DE, ... Knapp SJ (2021). Social network analysis of the genealogy of strawberry: retracing the wild roots of heirloom and modern cultivars. G3 Genes/Genomes/Genetics 11(3):jkab015. https://doi.org/10.1093/g3journal/jkab015
Powers L (1944). Meiotic studies of crosses between Fragaria ovalis and F. ananassa. Journal of Agricultural Research 69(11):435-448.
Prochnow L, Sánchez-Sevilla JF, Dubbini M, … Castillejos C (2025). Regulatory landscape for plant genetic resources: germplasm conservation and plant variety rights in the berry sector in Europe. Genetic Resources and Crop Evolution https://doi.org/10.1007/s10722-025-02553-2
Rios SA (2007). Melhoramento genético do morangueiro [Genetic improvement of strawberry plants]. Informe Agropecuário 28(1):14-19.
Salinas NR, Zurn JD, Mathey M, Mookerjee S, Denoyes B, Perrotte AP, … Bassil NV (2017). Validation of molecular markers associated with perpetual flowering in Octoploid Fragaria germplasm. Molecular Breeding 37(5):70. https://doi.org/10.1007/s11032-017-0672-2
Scott DH, Lawrence FJ (1975). Strawberries. In: Janick J, Moore JN (Eds). Advances in fruit breeding. Purdue University Press, West Lafayette pp 71-97.
Sevilla E, Sevilla A (2021). Strawberry.In Thurner M, Pimentel J (Eds). New world objects of knowledge. a cabinet of curiosities. Publisher: University of London Press pp.207-211
Shalizi MN, Isik F (2019). Genetic parameter estimates and GxE interaction in a large cloned population of Pinus taeda L. Tree genetics & genomes 15(3):46. https://doi.org/10.1007/s11295-019-1352-7
Smith AB, Cullis BR, Thompson R (2005). The analysis of crop cultivar breeding and evaluation trials: an overview of current mixed model approaches. The Journal of Agricultural Science 143(6):449-462. https://doi.org/10.1017/S0021859605005587
Smith AN (2022). Characterizing the impact of postharvest temperature stress on polyphenol profiles of red and white-fruited strawberry cultivars. MS Thesis. Univerisy of South Florida, Tampa https://digitalcommons.usf.edu/etd/9465
Sparacino A, Ollani S, Baima L, Oliviero M, Borra D, Rui M, Mastromonaco G (2024). Analyzing strawberry preferences: best–worst scaling methodology and purchase styles. Foods 13(10):1474. https://doi.org/10.3390/foods13101474
Tanaka MAS, Passos FA, Binotti CS, Novais AJ (1999). Variabilidade patogênica de isolados de Colletotrichum acutatum e C. fragariae em rizomas e pecíolos de morangueiro [Pathogenic variability of Colletotrichum acutatum and C. fragariae isolates in strawberry rhizomes and petioles]. Summa Phytopathologica 25:303-307.
Temocico G, Sturzeanu M (2018). Soiuri de căpșun cultivate în România [Strawberry varieties grown in Romania]. Invel, Ilfov, Romania
Temocico G, Sturzeanu M, Ion V, Cristea S (2019). Evaluation of strawberry fruit quality for new selections and cultivars. Romanian Biotechnological Letters 24(4):742-748. https://doi.org/10.25083/rbl/24.4/742.748
Tudor V, Asănică A, Neagu T (2014). First results of some day-neutral strawberry cultivars behavior in the Bucharest area conditions. Scientific Papers. Series B. Horticulture 58(1):101-106. https://horticulturejournal.usamv.ro/pdf/2014/art17.pdf
Tudor-Radu M, Vîjan LE, Tudor-Radu CM, Tița I, Sima R, Mitrea R (2016). Assessment of ascorbic acid, polyphenols, flavonoids, anthocyanins and carotenoids content in tomato fruits. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 44(2):477-483. https://doi.org/10.15835/nbha44210332
Ullah I, Demirsoy H, Soysal D, Lizalo A, Doğan DE, Demirsoy L (2024). Evaluation of strawberry cultivars based on growth-related attributes. Applied Fruit Science 66(2) 431-439. https://doi.org/10.1007/s10341-023-01025-0
Vîjan LE, Mazilu IC, Enache C, Enache S, Topală CM (2023). Botanical origin influence on some honey physicochemical characteristics and antioxidant properties. Foods 12(11):2134. https://doi.org/10.3390/foods12112134
Zoratti L, Palmieri L, Jaakola L, Häggman H (2015). Genetic diversity and population structure of an important wild berry crop. AoB Plants 7:plv117. https://doi.org/10.1093/aobpla/plv117
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Monica STURZEANU, Oana HERA, Mădălina MILITARU, Loredana E. VÎJAN

This work is licensed under a Creative Commons Attribution 4.0 International License.
License:

Open Access Journal:
The journal allows the author(s) to retain publishing rights without restriction. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.









.png)






