Johnsongrass (Sorghum halepense (L.) Moench) resistance to cycloxydim, fluazifop and propaquizafop and its impact on growth rate

Aristeidis P. PAPAPANAGIOTOU; Ioannis VASILAKOGLOU; Maria V. ALVANOU; Ioannis A. GIANTSIS; Ilias G. ELEFTHEROHORINOS

doi:10.15835/nbha53314605

Authors

Aristeidis P. PAPAPANAGIOTOU University of Western Macedonia, Department of Agriculture, Florina, 53100 (GR) https://orcid.org/0000-0002-9798-3853
Ioannis VASILAKOGLOU University of Thessaly, Department of Agriculture-Agrotechnology, Larissa, 41500 (GR) https://orcid.org/0000-0003-0393-2981
Maria V. ALVANOU University of Western Macedonia, Department of Agriculture, Florina, 53100; Aristotle University of Thessaloniki, Department of Agriculture, Thessaloniki, 54006 (GR) https://orcid.org/0000-0002-8645-0533
Ioannis A. GIANTSIS University of Western Macedonia, Department of Agriculture, Florina, 53100; Aristotle University of Thessaloniki, Department of Agriculture, Thessaloniki, 54006 (GR) https://orcid.org/0000-0002-6323-2955
Ilias G. ELEFTHEROHORINOS Aristotle University of Thessaloniki, Department of Field Crops and Ecology, Thessaloniki, 54006 (GR) https://orcid.org/0000-0003-4074-8235

DOI:

https://doi.org/10.15835/nbha53314605

Keywords:

ACCase gene sequence, ACCase inhibitors, cycloxydim, target-site resistance, weed growth rate

Abstract

One putative-resistant (R) johnsongrass (Sorghum halepense L. Moench) population, originating from a cotton monoculture field in northern Greece, was evaluated for the possible evolution of cross-resistance to acetyl-CoA carboxylase (ACCase)- and multiple resistance to acetolactate synthase (ALS)-inhibiting herbicides, and to elucidate the levels and underlying mechanisms of resistance. Whole-plant rate-response assays showed that the R population was highly cross-resistant to the post-emergence applied ACCase-inhibiting herbicides fluazifop-P-butyl, propaquizafop (aryloxyphenoxypropionates) and cycloxydim (cyclohexanedione), but susceptible to the ACCase-inhibitor clethodim (cyclohexanedione) and the ALS-inhibitor nicosulfuron. The analysis of the ACCase gene sequence revealed a point mutation (ATA to WTA/TTA) at 1781 residue in the CT domain of ACCase, resulting in an amino acid substitution from isoleucine (Ile) to leucine (Leu). However, all sequenced plants of the S johnsongrass population were found with the wild-type allele encoding Ile-1781. The R johnsongrass population, grown without competition, produced more fresh weight, rhizome biomass and number of panicles than the S population. These findings indicate clearly that the R johnsongrass population has evolved target-site cross-resistance to three ACCase-inhibitors that increased most of its growth traits as compared with the S population, suggesting a fitness advantage associated with the ACCase Leu-1781 mutation.

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