Enhanced accumulation of total phenolic content and rosmarinic acid in cell suspension cultures of Ehretia asperula using elicitors and precursors

Tuan Trong TRAN; Thao Thu Vo NGUYEN; Hang Thu Thi NGUYEN; Thuy Kim Thi DANG; Trang Huyen Thi NGUYEN; Giap Dang DO; Huong Thi TRINH

doi:10.15835/nbha53314521

Authors

Tuan Trong TRAN Vietnam Academy of Science and Technology (VAST), Institute of Life Sciences, Plant Biotechnology and Agriculture Department, 9/621 Vo Nguyen Giap street, Linh Xuan ward, HCM City, 700000 (VN) https://orcid.org/0000-0003-1077-7377
Thao Thu Vo NGUYEN Vietnam Academy of Science and Technology (VAST), Institute of Life Sciences, Plant Biotechnology and Agriculture Department, 9/621 Vo Nguyen Giap street, Linh Xuan ward, HCM City, 700000 (VN)
Hang Thu Thi NGUYEN Vietnam Academy of Science and Technology (VAST), Institute of Life Sciences, Plant Biotechnology and Agriculture Department, 9/621 Vo Nguyen Giap street, Linh Xuan ward, HCM City, 700000 (VN) https://orcid.org/0009-0007-4786-3849
Thuy Kim Thi DANG Vietnam Academy of Science and Technology (VAST), Institute of Life Sciences, Plant Biotechnology and Agriculture Department, 9/621 Vo Nguyen Giap street, Linh Xuan ward, HCM City, 700000 (VN) https://orcid.org/0000-0002-4212-6942
Trang Huyen Thi NGUYEN Vietnam Academy of Science and Technology (VAST), Institute of Life Sciences, Plant Biotechnology and Agriculture Department, 9/621 Vo Nguyen Giap street, Linh Xuan ward, HCM City, 700000 (VN) https://orcid.org/0009-0009-9561-0146
Giap Dang DO Vietnam Academy of Science and Technology (VAST), Institute of Life Sciences, Plant Biotechnology and Agriculture Department, 9/621 Vo Nguyen Giap street, Linh Xuan ward, HCM City, 700000 (VN) https://orcid.org/0000-0002-2052-4560
Huong Thi TRINH Ho Chi Minh City University of Industry and Trade, Faculty of Biology and Environment, 140 Le Trong Tan street, Tay Thanh ward, HCM City, 700000 (VN) https://orcid.org/0000-0002-5940-6588

DOI:

https://doi.org/10.15835/nbha53314521

Keywords:

L-tyrosine, L-phenylalanine, naphthalene acetic acid, salicylic acid, yeast extract

Abstract

Ehretia asperula, a Vietnamese endemic species known as Xạ Đen, has traditional medicinal applications for conditions such as hepatitis, liver cirrhosis, and cancer. Plant cell suspension cultures provide an alternative approach for producing secondary metabolites. This study focuses on the effects of precursors and elicitors on the accumulation of total phenolic and rosmarinic acid, one of the bioactive compounds presents in E. asperula. Suspension cultures were established using Gamborg (B5) medium supplemented with 1.5 mg L^-1 naphthalene acetic acid. Precursors, including L-tyrosine and L-phenylalanine, and elicitors, such as salicylic acid and yeast extract, were added to the culture medium at different concentrations to examine their effects on total phenolic and rosmarinic acid production. The results indicated that precursors and elicitors differentially influenced the accumulation of secondary metabolites and the growth of E. asperula cells. Notably, L-tyrosine was more effective than L-phenylalanine in enhancing biomass and secondary metabolite production after 15 days of culture, with the highest yield observed at 0.5 mg L^-1 L-tyrosine. Between the two elicitors tested, yeast extract significantly promoted the production of phenolic compounds and rosmarinic acid, with the highest levels recorded after 12 hours of treatment with 1.0 g L^-1 yeast extract. Overall, the findings highlight the potential of E. asperula cell suspension cultures as a substantial source of rosmarinic acid production. The protocol developed in this study offers a promising solution to address the challenges of large-scale production and provides a potential source of raw materials for pharmaceutical, cosmetic, and functional food industries.

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