Exogenous Spermidine (Spd) alleviates NaCl-induced injury effects by improving photosynthesis and oxidative stress tolerance in Brassica napus seedlings

Tian Yuan XUE; He Ping WAN; Xiao Ming WU; Li LIU; Jing Dong CHEN; Yi YU; Xi Gang DAI; Yuan Huo DONG; Chang Li ZENG

doi:10.15835/nbha53314420

Authors

Tian Yuan XUE Jianghan University, College of Life Sciences, Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, Wuhan, 430056 (CN)
He Ping WAN Jianghan University, College of Life Sciences, Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, Wuhan, 430056 (CN)
Xiao Ming WU Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430078 (CN)
Li LIU Jianghan University, College of Life Sciences, Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, Wuhan, 430056 (CN)
Jing Dong CHEN Jianghan University, College of Life Sciences, Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, Wuhan, 430056 (CN)
Yi YU Jianghan University, College of Life Sciences, Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, Wuhan, 430056 (CN)
Xi Gang DAI Jianghan University, College of Life Sciences, Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, Wuhan, 430056 (CN)
Yuan Huo DONG Jianghan University, College of Life Sciences, Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, Wuhan, 430056 (CN)
Chang Li ZENG Jianghan University, College of Life Sciences, Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, Wuhan, 430056 (CN)

DOI:

https://doi.org/10.15835/nbha53314420

Keywords:

Brassica napus, photosynthesis, salt stress, spermidine, stomatal conductance

Abstract

To better understand the mechanism of exogenous application of Spermidine (Spd) to enhance the salt tolerance of rapeseed (Brassica napus L.). The seedlings of rapeseed cultivar ‘Zheyou-18’ were treated with different concentrations of spermidine under 200 mM NaCl. The portable photosynthetic system flame, photometer and portable fluorometer were used to determine the gas-exchange parameters, ion content and Chlorophyll fluorescence. A spectrophotometer was used to determine the activities of SOD (Superoxide Dismutase), POD (Peroxidase), CAT (Catalase), and APX (Ascorbate Peroxidase). Our study revealed that different concentrations of exogenous Spd can alleviate the harmful effect caused by NaCl stress in rapeseed, and inhibition in rapeseed seedling growth was significantly alleviated with the application of 160 mg·L^-1spermidine under 200 mM NaCl, which is reflected in dry and fresh weight. The Spd treatment further enhanced the photosynthetic efficiency of rapeseed leaves, which is reflected in the changes in gas exchange parameters Pn (Photosynthetic rate), Ci (Intercellular CO₂ concentration), Tr (Transpiration rate), Gs (Stomatal conductance), Ls (stomatal limitation), WUE (Water Use Efficiency) and chlorophyll fluorescence parameters (Fv/Fm, ΦPSII, qP, NPQ), and antioxidant enzymes activity (SOD, POD, CAT, APX) which in turn reduced the level of active oxygen (H₂O₂, O₂^-). This study also indicated the Spd treatment reduced the absorption of Na⁺and increased the absoraption of K⁺ and Ca²⁺. The physiological experiments demonstrate that exogenous Spd enhances salt tolerance in rapeseed under NaCl stress through multiple mechanisms, including improved photosynthetic efficiency, maintenance of cellular ion homeostasis, and reduced reactive oxygen species (ROS) levels.

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