Biochemical attributes Portulaca oleracea as affected by manganese oxide and magnesium oxide nanoparticles

Mahshad MOVAHEDI; Sedigheh ARBABIAN; Ahmad MAJD; Golnaz TAJADOD; Sayeh Jafari MARANDI

doi:10.15835/nbha53114198

Authors

Mahshad MOVAHEDI Islamic Azad University, Department of Biology, Science and Research Branch, Tehran (IR)
Sedigheh ARBABIAN Islamic Azad University, Tehran North Branch, Faculty of Life Science, Department of Biology, Tehran (IR)
Ahmad MAJD Islamic Azad University, Tehran North Branch, Faculty of Life Science, Department of Biology, Tehran (IR)
Golnaz TAJADOD Islamic Azad University, Tehran North Branch, Faculty of Life Science, Department of Biology, Tehran (IR)
Sayeh Jafari MARANDI Islamic Azad University, Tehran North Branch, Faculty of Life Science, Department of Biology, Tehran (IR)

DOI:

https://doi.org/10.15835/nbha53114198

Keywords:

anthocyanin, flavonoid, halophytes, nanoparticles, phenol

Abstract

Due to the limitation of freshwater resources and quality loss of soils, the use of halophyte plants is inevitable. Halophyte species such as purslane (Portulaca oleracea) are one of the important and rich sources of different metabolites, however, very few studies have been done in this regard. The application of metal nanoparticles in improving plant growth characteristics and also knowing the threshold of using these nanoparticles can lead to plant growth and development. Therefore, the present study is aimed at investigating the effect of manganese oxide (Mn₂O₃) and magnesium oxide (MgO) nanoparticles (NPs) on biochemical attributes and plant growth of purslane in a controlled condition based on a balanced completely random design. The treatments included Mg and Mn nanoparticles in three concentrations: 10, 100, and 500 mg L^-1, bulk metal Mg and Mn in three concentrations of 10, 100, and 500 mg L^-1, and a control treatment. The results showed that nano treatments had a significant effect on the concentration of phenol, flavonoid, anthocyanin, quercetin, half-maximal inhibitory concentration (IC₅₀), total fresh weight, and total dry weight. Except for quercetin, the results indicated a positive effect of Mg and Mn, especially in the form of nanoparticles, on the quality and quantity of purslane plants. Increasing the concentration of Mg and Mn metals in nanoparticle form did not increase IC₅₀, however, it was associated with an increase in this index in bulk form, which indicates a decrease in the negative effect of metals in nanoparticle form. Considering the salinization of water and soil resources and the importance of preventing environmental pollution, the use of Mg and Mn nanoparticles on halophyte species is very important; which requires more research.

References

Aini NS, Ansori ANM, Kharisma VD, Syadzha MF, Widyananda MH, Murtadlo AAA, ... Zainul R (2022). Potential roles of purslane (Portulaca oleracea L.) as antimetabolic syndrome: A review. Pharmacognosy Journal 14(3).: 710-714. http://dx.doi.org/10.5530/pj.2022.14.90

Alejandro S, Höller S, Meier B and Peiter E (2020) Manganese in plants: From acquisition to subcellular allocation. Frontiers in Plant Science 11:300. http://doi.org/10.3389/fpls.2020.00300

Ammulu AM, Viswanath VK, Giduturi AK, Vemuri OK, Mangamuri U, Poda S (2021). Phyto assisted synthesis of magnesium oxide nanoparticles from Pterocarpus marsupium Rox.b heartwood extract and its biomedical applications. Journal of Genetic Engineering and Biotechnology 19:1-18. https://doi.org/10.1186/S43141-021-00119-0

Bhardwaj AK, Arya G, Kumar R, Hamed L, Pirasteh-Anosheh H, Jasrotia P, Kashyapm PL, Singh GP (2022). Switching to nanonutrients for sustaining agroecosystems and environment: The challenges and benefits in moving up from ionic to particle feeding. Journal of Nanobiotechnology 20(1):19. https://doi.org/10.1186/s12951-021-01177-9

Burbulis IE, Winkel-Shirley B (1999). Interactions among enzymes of the Arabidopsis flavonoid biosynthetic pathway. Proceedings of the National Academy of Science USA 96(22):12929-34. http://doi.org/10.1073/pnas.96.22.12929.

Cai Y, Zheng Z, Zhao Y, Zhang Y, Guo S, Cui Z, Wang X (2018). Effects of molybdenum, selenium and manganese supplementation on the performance of anaerobic digestion and the characteristics of bacterial community in acidogenic stage. Bioresource Technology 266:166-175. https://doi.org/10.1016/j.biortech.2018.06.061

Ciscomani-Larios JP, Sánchez-Chávez E, Jacobo-Cuellar JL, Sáenz-Hidalgo HK, Orduño-Cruz N, Cruz-Alvarez O, Ávila-Quezada GD (2021). Biofortification efficiency with magnesium salts on the increase of bioactive compounds and antioxidant capacity in snap beans. Ciência Rural 51(6):e20200442 http://doi.org/10.1590/0103-8478cr20200442

Gautam A, Sharma P, Ashokhan S, Yaacob JS, Kumar V, Guleria P (2023). Magnesium oxide nanoparticles improved vegetative growth and enhanced productivity, biochemical potency and storage stability of harvested mustard seeds. Environmental Research 229:116023. https://doi.org/10.1016/j.envres.2023.116023

Giordano R, Saii Z, Fredsgaard M, Hulkko LSS, Poulsen TBG, Thomsen ME, … Stensballe A (2021). Pharmacological insights into halophyte bioactive extract action on anti-inflammatory, pain relief and antibiotics-type mechanisms. Molecules 26(11):3140. http://doi.org/10.3390/molecules26113140

Hussain G, Hadi F, Akbar F (2019). Magnesium oxide nanoparticles and thidiazuron enhance lead phytoaccumulation and antioxidative response in Raphanus sativus L. Environmental Science and Pollution Research 26:30333-30347. https://doi.org/10.1007/S11356-019-06206-7

Kiapour H, Moaveni P, Sani B, Rajabzadeh F, Mozafari H (2020). Investigating the effect of magnesium and iron oxide nanoparticles on the levels of enzymatic and non-enzymatic antioxidants in roselle. Journal of Medicinal plants and By-Product 9(1):19-31. https://doi.org/10.22092/JMPB.2020.122071

Krizek DT, Britz SJ, Mirecki RM (1998). Inhibitory effects of ambient levels of solar UV‐A and UV‐B radiation on growth of cv. New Red Fire lettuce. Physiologia Plantarum 103(1):1-7. http://dx.doi.org/10.1034/j.1399-3054.1998.1030101.x

Kumar A, Sreedharan S, Kashyap AK, Singh P, Ramchiary N (2022). A review on bioactive phytochemicals and ethnopharmacological potential of purslane (Portulaca oleracea L.). Heliyon 8:e08669 http://doi.org/10.1016/j.heliyon.2021.e08669

Lestari T, Nofianti T, Tuslinah L, Ruswanto R (2018). Total phenol, flavonoid, and anthocyanin content and antioxidant activity of Etlingera elatior extract and nanoparticle. Pharmaciana 8(1):145-156. https://doi.org/10.12928/PHARMACIANA.V8I1.7511

Lopes M, Sanches-Silva A, Castilho M, Cavaleiro C, Ramos F (2023). Halophytes as source of bioactive phenolic compounds and their potential applications. Critical Reviews in Food Science and Nutrition 63(8):1078-1101. http://doi.org/10.1080/10408398.2021.1959295.

Mohammed HA, Emwas AH, Khan RA (2023). Salt-tolerant plants, halophytes, as renewable natural resources for cancer prevention and treatment: roles of phenolics and flavonoids in immunomodulation and suppression of oxidative stress towards cancer management. International Journal of Molecular Sciences 24(6):5171. http://doi.org/10.3390/ijms24065171.

Pirasteh-Anosheh H, Samadi M, Kazemeini SA, Ozturk M, Ludwiczak A, Piernik A (2023). ROS homeostasis and antioxidants in the halophytic plants and seeds. Plants 12(17):3023. https://doi.org/10.3390/plants12173023

Popescu C, Popescu C, Manea S, Vladut V, Caba I, Covaliu IC, ... Lupuleasa D (2018). Study on Portulaca oleracea native species as vegetal source of omega-3 and omega-6 fatty acids. Membranes 19:21. https://doi.org/10.37358/RC.18.11.6665

Pungin A, Lartseva L, Loskutnikova V, Shakhov V, Krol O, Popova E, Kolomiets A, Nikolaeva N, Volodina A (2022). The content of certain groups of phenolic compounds and the biological activity of extracts of various halophyte parts of Spergularia marina (L.) Griseb. and Glaux maritima L. at different levels of soil salinization. Plants 11(13):1738. http://doi.org/10.3390/plants11131738.

Salehi H, Cheheregani Rad A, Raza A, Djalovic I, Prasad PVV (2023) The comparative effects of manganese nanoparticles and their counterparts (bulk and ionic) in Artemisia annua plants via seed priming and foliar application. Frontiers in Plant Science 13:1098772. http://doi.org/10.3389/fpls.2022.1098772

Seevers P, Daly J (1970). Studies on wheat stem rust resistance controlled at the Sr6 locus. II. Peroxidase activities. Phytopathology 60(11):1642-1647. https://doi.org/10.1094/Phyto-60-1642

Sinilal B, Ovadia R, Nissim-Levi A, Perl A, Carmeli-Weissberg M, Oren-Shamir M (20110. Increased accumulation and decreased catabolism of anthocyanins in red grape cell suspension culture following magnesium treatment. Planta 234: 61-71. https://doi.org/10.1007/s00425-011-1377-0.

Siriamornpun S, Suttajit M (2010). Microchemical components and antioxidant activity of different morphological parts of Thai wild purslane (Portulaca oleracea). Weed Science 58(3):182-188. https://doi.org/10.1614/WS-D-09-00073.1

Stanković M, Jakovljević D (2021). Phytochemical diversity of halophytes. In: Grigore MN (Ed). Handbook of Halophytes. Springer, Cham. https://doi.org/10.1007/978-3-030-57635-6_125

Wagner GJ (1979). Content and vacuole/extravacuole distribution of neutral sugars, free amino acids, and anthocyanin in protoplasts. Plant Physiology 64(1): 88-93. https://doi.org/10.1104/pp.64.1.88

Wang Y, Li J, Pan Y, Chen J, Liu Y (2023). Metabolic responses to manganese toxicity in soybean roots and leaves. Plants 12(20):3615. https://doi.org/10.3390/plants12203615

Xu Z, Zhou J, Ren T, Du H, Liu H, Li Y, Zhang C (2020). Salt stress decreases seedling growth and development but increases quercetin and kaempferol content in Apocynum venetum. Plant Biology 22(5):813-821. https://doi.org/10.1111/plb.13128

Xuan TD, Huong CT, Quan NV, Anh LH, Khanh TD, Rayee R (2022). Improvement of salinity tolerance in rice seedlings by exogenous magnesium sulfate application. Soil Systems 6(3):69.

Zwijnenburg MA (2021). The effect of particle size on the optical and electronic properties of magnesium oxide nanoparticles. Physical Chemistry Chemical Physics 23(38):21579-21590. http://doi.org/10.1039/D1CP02683F