Effects of different rootstocks on the growth physiology and enzyme activity of Artemisia selengensis

Sixiao HE; Mang XIA; Meizhu CHEN; Xiaoxiao DONG; Miao CHENG; Jingdong CHEN; Tianyuan XUE; Heping WAN; Yuanhuo DONG; Changli ZENG; Xigang DAI

doi:10.15835/nbha53114148

Authors

Sixiao HE Jianghan University, School of Life Science, Wuhan 430056 (CN)
Mang XIA Jianghan University, School of Life Science, Wuhan 430056 (CN)
Meizhu CHEN Jianghan University, School of Life Science, Wuhan 430056 (CN)
Xiaoxiao DONG Jianghan University, School of Life Science, Wuhan 430056 (CN)
Miao CHENG Jianghan University, School of Life Science, Wuhan 430056 (CN)
Jingdong CHEN Jianghan University, School of Life Science, Wuhan 430056 (CN)
Tianyuan XUE Jianghan University, School of Life Science, Wuhan 430056 (CN)
Heping WAN Jianghan University, School of Life Science, Wuhan 430056; Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, Wuhan 430056 (CN)
Yuanhuo DONG Jianghan University, School of Life Science, Wuhan 430056; Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, Wuhan 430056 (CN)
Changli ZENG Jianghan University, School of Life Science, Wuhan 430056; Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, Wuhan 430056 (CN)
Xigang DAI Jianghan University, School of Life Science, Wuhan 430056; Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, Wuhan 430056 (CN)

DOI:

https://doi.org/10.15835/nbha53114148

Keywords:

Artemisia selengensis, grafting, growth nutrients, graft combination, growth physiology

Abstract

In this experiment, wild-type Artemisia selengensis, Chrysanthemum, Artemisia annua and mugwort were used as rootstocks, while cultivated A. selengensis served as the scion. Cleft grafting was identified as the most effective method. The highest survival rate was observed when wild-type A. selengensis was used as the rootstock, reaching 92.5% on the 12th day post-grafting, with quicker callus formation than in other combinations. The combination of wild-type A. selengensis rootstock and cultivated A. selengensis scion demonstrated the greatest grafting compatibility, whereas the pairing of cultivated A. selengensis with chrysanthemum showed the lowest. During the healing phase, analysis revealed that in compatible graft combinations, soluble sugar levels increased, with a significant rise in soluble protein levels. Following callus formation, levels of superoxide dismutase (SOD), peroxidase (POD), and phenylalanine ammonia-lyase (PAL) declined. Hence, soluble sugars, SOD, POD, and PAL may act as indicators of grafting compatibility in A. selengensis. Weekly elongation measurements from 21 to 49 days post-grafting indicated that combinations rooted in chrysanthemum exhibited markedly greater elongation from 35 to 49 days than other pairs. Comparisons of soluble proteins, sugars, vitamin C, and flavonoids in the scion on days 49, 70, and 91 post-grafting with those in ungrafted plants suggested that different rootstocks differentially influenced nutrient and metabolite accumulation in A. selengensis. These findings suggest that future research could explore the mechanisms underlying these changes during the grafting process. Integrating molecular biology and metabolomics techniques will aid in elucidating specific regulatory mechanisms affected by grafting in Artemisia species, potentially enhancing the content of nutrients or bioactive compounds. This could provide theoretical support for biopharmaceutical applications and propose new methods for improving the quality of Artemisia germplasm resources.

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