Antifungal nanoformulation of botanical anthraquinone and TiO2 against melon phytopathogenic fungi: preparation, in vitro bioassays and field test
DOI:
https://doi.org/10.15835/nbha53114108Keywords:
anthraquinone, antifungal activity, field test, melon, nanoemulsion, TiO2 nanoparticlesAbstract
Anthraquinones from Polygonum cuspidatum are well-known antimicrobial botanical compounds against several plant pathogenic agents that cause plant disease. In this study, for the first time, the anthraquinone-rich extract of P. cuspitaum and TiO2 nanoparticles (average droplet size of 103.9 nm) together with surfactants and cosurfactants were fabricated in the type of emulsifiable concentrate (ATEC) that form spontaneous nanoemulsions (ATNE) in water and evaluated its antifungal efficacy against the melon fungal diseases. The morphology of ATNE nanodroplets observed using SEM and TEM showed that ATNE droplets were spherical in shape. ATEC showed excellent antifungal activity against various melon phytopathogenic fungi. At a concentration of 0.5%, the formulation completely inhibited the mycelial growth of Botrytis cinerea, Phytophthora spp., Fusarium oxysporum, Colletotrichum spp., Sclerotium spp., and Rhizoctonia spp., and the germination of Eryshipe cichorasearum spores. In the field test, ATNE nanoformulations were evaluated for their control efficacy against powdery mildew by spraying on young honeydew melon plants. As a result, ATNE (1:400) with an average droplet size of 484.4 nm exhibited the best suppression (control efficacy of 62.34 %) against powdery mildew at 10 days after spray and showed no phytotoxicity on the test plants. The study results confirmed the potential of ATEC against various melon phytopathogenic fungi. In particular, under organic agriculture practice, the nanoemulsion ATNE could effectively control powdery mildew caused by E. cichorasearum in honeydew melon fields.
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Copyright (c) 2024 Duong Quang PHAM, Tan Van CHU, Hiep Thi PHAN, Son Vu NGUYEN, Tam The LE, De Quang TRAN, Hung Khac NGUYEN, Anh Thi Kieu VO, Lam Dai TRAN, Khanh Ngoc TRAN, Hoang Dinh VU, Quang Dang LE
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