Physiological and anatomical adaptations of rice (Oryza sativa L.) grown under drought stress

Narisa KUNPRATUM; Attachai TRUNJARUEN; Chonnakarn KUNGLA; Pitakpong MANEERATTANARUNGROJ; Ploinapat MAHATTHANAPHATCHARAKUN; Worasitikulya TARATIMA

doi:10.15835/nbha53114060

Authors

Narisa KUNPRATUM Naresuan University, Department of Biology, Faculty of Science, Phitsanulok 65000 (TH)
Attachai TRUNJARUEN Mahidol University, Amnatcharoen Campus, Amnatcharoen 37000 (TH)
Chonnakarn KUNGLA Khon Kaen University, Department of Biology, Faculty of Science, Khon Kaen 40002 (TH)
Pitakpong MANEERATTANARUNGROJ Khon Kaen University, Faculty of Veterinary Medicine, Khon Kaen 40002 (TH)
Ploinapat MAHATTHANAPHATCHARAKUN Khon Kaen University, Department of Biology, Faculty of Science, Khon Kaen 40002 (TH)
Worasitikulya TARATIMA Khon Kaen University, Department of Biology, Faculty of Science, Khon Kaen 40002 (TH)

DOI:

https://doi.org/10.15835/nbha53114060

Keywords:

anatomical adaptation, drought stress, physiological characteristics, water deficit

Abstract

Drought stress can affect significant productivity and quality attributes in rice. This research assessed the impact of drought stress on the physiological and anatomical adaptations of ‘Tubtim Chumphae’ rice. Seedlings were cultivated for 45 days in soil before being subjected to drought stress. The seedlings were divided into two groups as full water capacity treatment and drought stress treatment for 21 days before rewatering for 10 days. Dehydration from drought stress reduced rice seedling plant height, tiller number, leaf size, and fresh and dry weight while leaf rolling score increased. The recovery process from drought stress impacted the physiological characteristics. Relative water content and chlorophyll fluorescence decreased while green intensity (SPAD value), chlorophyll content, electrolyte leakage percentage, and malondialdehyde (MDA) content increased. Anatomical studies using free-hand section and peeling techniques revealed that water deficit reduced vascular bundle size, bulliform cell size, stomatal size, and epidermal cell (short cell) size while leaf thickness, cuticle and cell wall thickness and bulliform cell number increased. Our results provide useful information on rice seedling adaptation and response to drought for use in further studies of ‘Tubtim Chumphae’ rice and other cultivars.

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