Changes in composition, yield, antimicrobial and antioxidant activities of the Ocimum tenuiflorum L. essential oils as affected by fertilizers

Authors

  • Quyen Thi HA Vietnam National University Hanoi, VNU University of Engineering and Technology, Faculty of Agricultural Technology, 144 Xuan Thuy, Ha Noi 10053 (VN)
  • Thi Thu Ha CHU Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Ha Noi 10072; Graduate University of Science and Technology, VAST, 18 Hoang Quoc Viet, Ha Noi 10072 (VN) https://orcid.org/0000-0002-3479-9469
  • Nghiem Thi VU Institute of Materials Science, VAST, 18 Hoang Quoc Viet, Ha Noi 10072 (VN)
  • Thuy Thi Thu DINH Institute of Natural Product Chemistry, VAST, 18 Hoang Quoc Viet, Ha Noi 10072 (VN)
  • Phat Tien DO Graduate University of Science and Technology, VAST, 18 Hoang Quoc Viet, Ha Noi 10072; Institute of Biotechnology, VAST, 18 Hoang Quoc Viet, Ha Noi 10072 (VN)
  • Ha Duc CHU Vietnam National University Hanoi, VNU University of Engineering and Technology, Faculty of Agricultural Technology, 144 Xuan Thuy, Ha Noi 10053 (VN)
  • Phuong Lan HOANG Vietnam National University of Agriculture, Trau Quy, Gia Lam, Hanoi 10056 (VN)
  • Uyen Ngoc Lam PHI The Olympia School, Trung Van Urban Area, Nam Tu Liem District, Hanoi 10053 (VN)
  • William N. SETZER University of Alabama in Huntsville, Department of Chemistry, Huntsville, AL 35899; Aromatic Plant Research Center, 230 N 1200 E, Suite 100, Lehi, UT 84043 (US)

DOI:

https://doi.org/10.15835/nbha52313895

Keywords:

gas-chromatography, eugenol, (E)-β-caryophyllene, essential oil composition, fertilizers, Ocimum tenuiflorum L., tulsi

Abstract

The purpose of this study is to evaluate the impact of multifunctional microbial fertilizer and inorganic macronutrient fertilizers N, P, K on plant biomass, essential oil yield, chemical composition, antimicrobial and antioxidant activities of tulsi (Ocimum tenuiflorum L.) grown in Hanoi, Vietnam. Among the four formulas tested, F2 emerged as the most favourable, comprising a basal fertilization of 10 tons of decomposed manure, 15 kg of multifunctional microbial fertilizer, 150 kg of P fertilizer, and 75 kg of K fertilizer, together with top dressing of 200 kg of N fertilizer and 75 kg of K fertilizer per hectare.  This formula yielded the highest tulsi dry biomass (4.73 ton/ha), essential oil yield (0.831%), and essential oil production (39.29 L/ha), which were significantly different (p < 0.001) from those of the remaining formulas. In addition, the concentration of the essential oil main compound - eugenol (63.1%) of tulsi in F2 was also the highest, which may be the cause of the strongest antibacterial activity against Bacillus subtilis, Escherichia coli (IC50 of 320, 273 µg/mL, respectively), and antifungal activity against Candida albicans (IC50 of 400 µg/mL), as well as antioxidant activity (IC50 of 0.165 µg/mL) compared to the other formulas tested. The results suggested the role of both multifunctional microbial fertilizer and inorganic macronutrient fertilizers N, P, K on growth and essential oil biosynthesis in tulsi. These findings are important in identifying the most efficient fertilization formula for cultivating tulsi, serving the needs of the cosmetic and medicinal industries.

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Published

2024-08-20

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HA, Q. T., CHU, T. T. H., VU, N. T., DINH, T. T. T., DO, P. T., CHU, H. D., HOANG, P. L., PHI, U. N. L., & SETZER, W. N. (2024). Changes in composition, yield, antimicrobial and antioxidant activities of the Ocimum tenuiflorum L. essential oils as affected by fertilizers. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 52(3), 13895. https://doi.org/10.15835/nbha52313895

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Vol. 52 No. 3 (2024)

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Research Articles
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DOI: 10.15835/nbha52313895

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Copyright (c) 2024 Quyen Thi HA, Thi Thu Ha CHU, Nghiem Thi VU, Thuy Thi Thu DINH, Phat Tien DO, Ha Duc CHU, Phuong Lan HOANG, Uyen Ngoc Lam PHI, William N. SETZER

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