Differential physiological responses to salt stress in rice landraces in Thailand
DOI:
https://doi.org/10.15835/nbha52313778Keywords:
biodiversity, landrace rice, osmotic adjustment, proline, salt stress, salt toleranceAbstract
Soil salinity is a limitation factor for rice cultivation in the north-eastern Thailand. Understanding the mechanisms of salt stress tolerance is critical for maintaining or improving crop yield under salt stress. Over the past ten years, many landrace rice varieties have been examined for genetic diversity. To utilize the richness of natural resources and to explore novel genetic resources for developing a new variety, evaluating local rice varieties for salinity is still needed. This study investigated the physiological and biochemical responses of 8 landrace rice varieties in comparison to ‘Pokkali’, the standard salt-tolerant rice genotype, to clarify the major salt tolerance mechanisms in the landraces found in Thailand. After being exposed to 120 mM NaCl for 7 days, ‘Pokkali’ expressed physiological parameters in response to salt stress which are indicative of salt tolerance ability including low growth reduction, low Na+/K+ ratio, low chlorophyll degradation and low membrane integrity. In contrast, the landrace varieties displayed varying patterns of response. No landrace variety showed an outstanding ion exclusion mechanism as ‘Pokkali’, as evidenced by the fact that all landrace varieties showed approximately two times higher Na+/K+ ratios than ‘Pokkali’. However, it was found that ‘Surin’ had a similar salt stress response to ‘Pokkali’ (low growth reduction, low chlorophyll degradation, low membrane integrity), except for the markedly increased proline and sugar accumulation, suggesting an osmotic adjustment mechanism. Therefore, this variety could be a potential genetic resource to be developed as a donor for the osmotic adjustment trait to improve salt-tolerant rice in the future.
References
Ali A, Petrov V, Yun D, Gechev T (2023). Revisiting plant salt tolerance: novel components of the SOS pathway. Trends in Plant Science 28(9):1060-1069. https://doi.org/10.1016/j.tplants.2023.04.003
Akyol TY, Yilmaz O, Uzilday B, Uzilday RÖ, Ismail T (2020). Plant response to salinity: an analysis of ROS formation, signaling, and antioxidant defense. Turkish Journal of Botany 44(1):1-13. https://doi.org/10.3906/bot-1911-15
Atta K, Mondal S, Gorai S, Singh AP, Kumari A, Ghosh T, … Jespersen D (2023). Impacts of salinity stress on crop plants: improving salt tolerance through genetic and molecular dissection. Frontiers in Plant Science 14:1241736. https://doi.org/10.3389/fpls.2023.1241736
Arnon DT (1949). Copper enzyme in isolated chloroplasts polyphenoloxidase in Beta vulgari. Plant Physiology 24:1-15.
Arunin S, Pongwichian P (2015). Salt-afected soils and management in Thailand. Bulletin of the Society of Sea Water Science 69:319-325. https://doi.org/10.11457/swsj.69.319
Bates LS, Waldren RP, Teare ID (1973). Rapid determination of free proline for water-stress studies. Plant and Soil 39:205-207. https://doi.org/10.1007/BF00018060
Chakraborty K, Mondal S, Ray S, Samal P, Pradhan B, Chattopadhyay K, … Sarkar RK (2020) Tissue tolerance coupled with ionic discrimination can potentially minimize the energy cost of salinity tolerance in rice. Frontiers in Plant Science 11:265. https://doi.org/10.3389/fpls.2020.00265
Conde A, Chaves MM, Gerós H (2011). Membrane transport, sensing and signaling in plant adaptation to environmental stress. Plant and Cell Physiology 52(9):1583-602. https://doi.org/10.1093/pcp/pcr107
Dubey RS, Singh AK (1999) Salinity induces accumulation of soluble sugars and alters the activity of sugar metabolizing enzymes in rice plants. Plant Biology 42:233.
Filek M, Walas S, Mrowiec H, Rudolphy-SkÓrska E, Sieprawska A, Biesaga- Kościelniak J (2012). Membrane permeability and micro- and macro element accumulation in spring wheat cultivars during the short-term effect of salinity- and PEG-induced water stress. Acta Physiologiae Plantarum 34:985-995. https://doi.org/10.1007/s11738-011-0895-5
Gerona MEB, Deocampo MP, Egdane JA, Ismail AM, Dionisio-Sese ML (2019). Physiological responses of contrasting rice genotypes to salt stress at reproductive stage. Rice Science 26(4):207-219. https://doi.org/10.1016/j.rsci.2019.05.001
Ghosh UK, Islam MN, Siddiqui MN, Cao X, Khan MAR (2022). Proline, a multifaceted signalling molecule in plant responses to abiotic stress: understanding the physiological mechanisms. Plant Biology (Stuttg) 24:227-239. https://doi.org/10.1111/plb.13363
Guo Q, Liu L, Barkla JB (2019). Membrane lipid remodeling in response to salinity. International Journal of Molecular Sciences 20(17):4264. https://doi.org/10.3390/ijms20174264
Huang L, Wu DZ, Zhang GP (2020). Advances in studies on ion transporters involved in salt tolerance and breeding crop cultivars with high salt tolerance. Journal of Zhejiang University-SCIENCE B 21:426-441. https://doi.org/10.1631/jzus.B1900510
Hasanuzzaman M, Fujita M (2022). Plant responses and tolerance to salt stress: physiological and molecular interventions. International Journal of Molecular Sciences 23(9):4810. https://doi.org/10.3390/ijms23094810
Karim R, Bouchra B, Fatima G, Abdelkarim FM, Laila S (2021). Plant NHX antiporters: from function to biotechnological application, with case study. Current Protein & Peptide Science 22:60-73. https://doi.org/10.2174/1389203721666201103085151
Khotasena S, Sanitchon J, Chankaew S, Monkham T (2022). The basic vegetative phase and photoperiod sensitivity index as the major criteria for indigenous upland rice production in Thailand under unpredictable conditions. Agronomy 12(4):957. https://doi.org/10.3390/agronomy12040957
Kwanwah MR, Wongsa T, Monkham T, Chankaew S, Falab S, Sanitchon J (2020). Thai indigenous lowland rice germplasms: sources of bacterial blight disease resistance and agronomic attributes. AGRIVITA, Journal of Agricultural Science 42(2):367-380. http://doi.org/10.17503/agrivita.v42i2.2738
Ma NL, Lah WAC, Kadir NA, Mustaqim M, Rahmat Z, Ahmad A, … Ismail MR (2018). Susceptibility and tolerance of rice crop to salt threat: physiological and metabolic inspections. PLoS One 13(2):e0192732. https://doi.org/10.1371/journal.pone.0192732
Mahlooji M, Seyed Sharifi R, Razmjoo J, Sabzalian MR, Sedghi M (2018). Effect of salt stress on photosynthesis and physiological parameters of three contrasting barley genotypes. Photosynthetica 56(2):549-556. https://doi.org/10.1007/s11099-017-0699-y
Nounjan N, Theerakulpisut P (2021). Physiological evaluation for salt tolerance in green and purple leaf color rice cultivars at seedling stage. Physiology and Molecular Biology of Plants 27:2819-2832. https://doi.org/10.1007/s12298-021-01114-y
Nounjan N, Mahakham W, Siangliw J L, Toojinda T, Theerakulpisut P (2020). Chlorophyll retention and high photosynthetic performance contribute to salinity tolerance in rice carrying drought tolerance QTL. Agriculture 10(12):620. https://doi.org/10.3390/agriculture10120620
Nounjan N, Chansongkrow P, Charoensawan V, Siangliw J L, Toojinda T, Chadchawan S, Theerakulpisut P (2018). High performance of photosynthesis and osmotic adjustment are associated with salt tolerance ability in rice carrying drought tolerance QTL: physiological and co-expression network analysis. Frontiers in Plant Science 9:1135. https://doi.org/10.3389/fpls.2018.01135
Office of Agriculture Economics (2016). Ministry of Agriculture and Cooperatives, Thailand. Retrieved 2019 October 30 from https://www.oae.go.th
Pamuta D, Siangliw M, Sanitchon J, Pengrat J, Siangliw JL, Toojinda T, Theerakulpisut P (2020). Photosynthetic performance in improved ‘KDML105’ Rice (Oryza sativa L.) lines containing drought and salt tolerance genes under drought and salt stress. Pertanika Journal of Tropical Agricultural Science. 43:4. https://doi.org/10.47836/pjtas.43.4.17
Pamuta D, Siangliw M, Sanitchon J, Pengrat J, Siangliw JL, Toojinda T, Theerakulpisut P (2021). Physio-biochemical traits in improved ‘KDML105’ jasmine rice lines containing drought and salt tolerance gene under drought and salt stress. Chilean Journal of Agricultural Research 82:97-110. http://dx.doi.org/10.4067/S0718-58392022000100097
Paudel D, Dhakal S, Parajuli S, Adhikari L, Peng Z, Qian Y, … Kannan B (2020). Chapter 38 - use of quantitative trait loci to develop stress tolerance in plants. In: Tripathi DK, Singh VP, Chauhan DK, Sharma S, Prasad SM, Dubey NK, Ramawat N (Eds). Plant life under changing environment. Academic Press, United States pp 917.
Prusty MR, Kim SR, Vinarao R, Entila F, Egdane J, Diaz MGQ, Jena KK (2018). Newly identified wild rice accessions conferring high salt tolerance might use a tissue tolerance mechanism in leaf. Frontiers in Plant Science 9:417. https://doi.org/10.3389/fpls.2018.00417
Pusadee T, Wongtamee A, Rerkasem B, Olsen KM, Jamjod S (2019). Farmers drive genetic diversity of Thai purple rice (Oryza sativa L.) landraces. Economic Botany 73: 76-85. https://doi.org/10.1007/s12231-018-9436-0
Rahiniza K, Adam MMA, Firdaus ARMS, Hamidah MS, Shahida H, Hidayah ZN, … Asfaliza R (2023). Chlorophyll index evaluation and selection of Malaysian salinity tolerant rice (Oryza sativa L.). Journal of Genetics Genomics and Plant Breeding 7:40-50
Rasel MM, Tahjib-Ul-Arif M, Hossain MA, Sayed MA, Hassan L (2020). Discerning of rice landraces (Oryza sativa L.) for morpho-physiological, antioxidant enzyme activity, and molecular markers’ responses to induced salt stress at the seedling stage. Journal of Plant Growth Regulation 39:41-59. https://doi.org/10.1007/s00344-019-09962-5
Roy SJ, Negrão S, Tester M (2014). Salt resistant crop plants. Current Opinion in Biotechnology 26:115-24. https://doi.org/10.1016/j.copbio.2013.12.004
Singh P, Choudhary KK, Chaudhary N, Gupta S, Sahu M, Tejaswini B, Sarkar S (2022). Salt stress resilience in plants mediated through osmolyte accumulation and its crosstalk mechanism with phytohormones. Frontiers in Plant Science 13:1006617. https://doi.org/10.3389/fpls.2022.1006617
Sheikh-Mohamadi MH, Etemadi N, Aalifar M, Pessarakli M (2022). Salt stress triggers augmented levels of Na+, K+ and ROS alters salt-related gene expression in leaves and roots of tall wheatgrass (Agropyron elongatum). Plant Physiology and Biochemistry 183:9-22. https://doi.org/10.1016/j.plaphy.2022.04.022
Sogir MA, Sharma AC, Mithila SS, Rasel M, Ferdausi A (2023). Morpho-molecular assessment of local rice (Oryza sativa L.) genotypes at seedling stage for salinity tolerance. Cereal Research Communications https://doi.org/10.1007/s42976-023-00438-7
Srivastava S, Sharma PK (2022). Morpho-physiological and biochemical tolerance mechanisms in two varieties of Oryza sativa to salinity. Russian Journal of Plant Physiology 69:37. https://doi.org/10.1134/S1021443722020194
Sun Y, Li J, Xing J, Yu X, Lu Y, Xu W, … Guo Z (2022). Evaluation of salt tolerance in common vetch (Vicia sativa L.) germplasms and the physiological responses to salt stress. Journal of Plant Physiology 278:153811. https://doi.org/10.1016/j.jplph.2022.153811
Tabassum R, Tahjib-Ul-Arif Md, Hasanuzzaman Md, Sohag AAM, Islam MdS, Shafi SMSH, … Hassan L (2021). Screening salt-tolerant rice at the seedling and reproductive stages: an effective and reliable approach. Environmental and Experimental Botany 192:104629. https://doi.org/10.1016/j.envexpbot.2021.104629
Vanavichit A, Kamolsukyeunyong W, Siangliw M, Siangliw JL, Traprab S, Ruengphayak S, … Tragoonrung S (2018). Thai Hom Mali Rice: origin and breeding for subsistence rainfed lowland rice system. Rice 11:20. https://doi.org/10.1186/s12284-018-0212-7
Yoshida S, Forno DA, Cock JH, Gomez KA (1976). Laboratory manual for physiological studies of rice. Los Banos: International Rice Research Institute.
Zahra N, Al Hinai MS, Hafeez MB, Rehman A, Wahid A, Siddique KHM, Farooq M (2022). Regulation of photosynthesis under salt stress and associated tolerance mechanisms. Plant Physiology and Biochemistry 178:55-69. https://doi.org/10.1016/j.plaphy.2022.03.003
Zelm EV, Zhang Y, Testerink C (2020). Salt tolerance mechanisms of plants. Annual Review of Plant Biology 71:403-433. https://doi.org/10.1146/annurev-arplant-050718-100005
Zulfiqar F, Akram NA, Ashraf M (2020). Osmoprotection in plants under abiotic stresses: new insights into a classical phenomenon. Planta 251:3. https://doi.org/10.1007/s00425-019-03293-1
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Piyada Theerakulpisut, Dr. Noppawan Nounjan, Asst. Prof. Jirawat Sanitchon, Dr. Dechudom Pamuta
This work is licensed under a Creative Commons Attribution 4.0 International License.
License:
Open Access Journal:
The journal allows the author(s) to retain publishing rights without restriction. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.